Oxytetracycline tablet production technology

Abstract

The invention discloses an oxytetracycline tablet production technology. A traditional oxytetracycline production method is wholly improved, bacterial strains are optimized and improved, spore is putunder a deuterium lamp to be irradiated under 250 to 300nm ultraviolet rays for 30 to 60s to be modified, and the modified bacterial strains are fermented to obtain oxytetracycline fermentation liquorwith a titer as 38000mu / mL; compared with traditional 6 to 8-day fermentation, not only is fermentation time greatly shortened, but also the titer of the oxytetracycline fermentation liquor is about13% higher than that of traditional fermentation, and a production efficiency is improved; most organic impurities in the obtained oxytetracycline fermentation liquor is removed by ultrafiltration, inorganic impurities are removed by nanofiltration, a crystallization speed is strictly controlled in a lateral crystallization process, and impurities which are difficult to be separated from oxytetracycline are removed; when the quality of a frozen and dried oxytetracycline pure product is detected by using a spectrophotometer, a purity of the obtained oxytetracycline pure product can be as high as 99.5%. The production technology has the advantages of strong practicability and application to industrial mass production.

Description

technical field

[0001] The invention belongs to the field of biopharmaceuticals, in particular to a production process of oxytetracycline tablets. Background technique

[0002] Oxytetracycline has a broad-spectrum anti-pathogenic microorganism effect, is a rapid bacteriostatic agent, and has a bactericidal effect on some bacteria at high concentrations. Its mechanism of action is that the drug can specifically bind to the A position of the ribosomal 30S subunit, preventing the connection of aminoacyl-tRNA at this position, thereby inhibiting the growth of the peptide chain and affecting the protein synthesis of bacteria or other pathogenic microorganisms. Oxytetracycline is effective against Staphylococcus aureus, Pneumococcus, Streptococcus pyogenes, Neisseria gonorrhoeae, Meningococcus, Escherichia coli, Bacillus aerogenes, Shigella, Yersinia, Listeria monocytogenes, etc. Strong antibacterial activity; in addition, oxytetracycline also has a strong effect on Rickettsia, m...

Examples

Embodiment 1

[0043] A kind of production technology of oxytetracycline sheet, specifically comprises the following steps:

[0044] (1) Preparation of fermentation broth;

[0045] Prepare oxytetracycline fermented liquid, select Streptomyces fistidis culture medium for use, the nitrogen source of described Streptomyces crackes culture medium is soybean cake powder, light yellow corn steep liquor, urea and egg white liquid, and carbon source is potato flour, dextrin ;Adopt purebred submerged, aeration and stirring three-stage fermentation culture method,

[0046] a, preparation of slanted spores: culture spore conditions: slanted spores are cultivated at a temperature of 35°C, humidity is 55%, and the incubation time is 96h; then the spores are placed under a deuterium lamp and irradiated for 30s under a 250-300nm ultraviolet light to modify, and the The modified spores are inoculated into the primary seed tank for cultivation;

[0047] b. Primary seed tank cultivation: the cultivation tem...

Embodiment 2

[0067] A kind of production technology of oxytetracycline sheet, specifically comprises the following steps:

[0068] (1) Preparation of fermentation broth;

[0069] Prepare oxytetracycline fermented liquid, select Streptomyces fistidis culture medium for use, the nitrogen source of described Streptomyces crackes culture medium is soybean cake powder, light yellow corn steep liquor, urea and egg white liquid, and carbon source is potato flour, dextrin ;Adopt purebred submerged, aeration and stirring three-stage fermentation culture method,

[0070] a, preparation of slant spores: culture spore conditions: the slant spores are cultivated at a temperature of 38°C, the humidity is 60%, and the cultivation time is 120h; then the spores are placed under a deuterium lamp and irradiated for 60s under a 250-300nm ultraviolet light for modification, and the The modified spores are inoculated into the primary seed tank for cultivation;

[0071] b. Primary seed tank cultivation: the cu...

Embodiment 3

[0091] A kind of production technology of oxytetracycline sheet, specifically comprises the following steps:

[0092] (1) Preparation of fermentation broth;

[0093] Prepare oxytetracycline fermented liquid, select Streptomyces fistidis culture medium for use, the nitrogen source of described Streptomyces crackes culture medium is soybean cake powder, light yellow corn steep liquor, urea and egg white liquid, and carbon source is potato flour, dextrin ;Adopt purebred submerged, aeration and stirring three-stage fermentation culture method,

[0094] a, preparation of slant spores: culture spore conditions: slant spores are cultivated at a temperature of 36°C, humidity is 57%, and the incubation time is 100h; then the spores are placed under a deuterium lamp and irradiated for 50s under a 250-300nm ultraviolet light to modify, and the The modified spores are inoculated into the primary seed tank for cultivation;

[0095] b. Primary seed tank cultivation: the cultivation temper...

technical field

[0001] The invention belongs to the field of biopharmaceuticals, in particular to a production process of oxytetracycline tablets. Background technique

[0002] Oxytetracycline has a broad-spectrum anti-pathogenic microorganism effect, is a rapid bacteriostatic agent, and has a bactericidal effect on some bacteria at high concentrations. Its mechanism of action is that the drug can specifically bind to the A position of the ribosomal 30S subunit, preventing the connection of aminoacyl-tRNA at this position, thereby inhibiting the growth of the peptide chain and affecting the protein synthesis of bacteria or other pathogenic microorganisms. Oxytetracycline is effective against Staphylococcus aureus, Pneumococcus, Streptococcus pyogenes, Neisseria gonorrhoeae, Meningococcus, Escherichia coli, Bacillus aerogenes, Shigella, Yersinia, Listeria monocytogenes, etc. Strong antibacterial activity; in addition, oxytetracycline also has a strong effect on Rickettsia, m...

Examples

Embodiment 1

[0043] A kind of production technology of oxytetracycline sheet, specifically comprises the following steps:

[0044] (1) Preparation of fermentation broth;

[0045] Prepare oxytetracycline fermented liquid, select Streptomyces fistidis culture medium for use, the nitrogen source of described Streptomyces crackes culture medium is soybean cake powder, light yellow corn steep liquor, urea and egg white liquid, and carbon source is potato flour, dextrin ;Adopt purebred submerged, aeration and stirring three-stage fermentation culture method,

[0046] a, preparation of slanted spores: culture spore conditions: slanted spores are cultivated at a temperature of 35°C, humidity is 55%, and the incubation time is 96h; then the spores are placed under a deuterium lamp and irradiated for 30s under a 250-300nm ultraviolet light to modify, and the The modified spores are inoculated into the primary seed tank for cultivation;

[0047] b. Primary seed tank cultivation: the cultivation tem...

Embodiment 2

[0067] A kind of production technology of oxytetracycline sheet, specifically comprises the following steps:

[0068] (1) Preparation of fermentation broth;

[0069] Prepare oxytetracycline fermented liquid, select Streptomyces fistidis culture medium for use, the nitrogen source of described Streptomyces crackes culture medium is soybean cake powder, light yellow corn steep liquor, urea and egg white liquid, and carbon source is potato flour, dextrin ;Adopt purebred submerged, aeration and stirring three-stage fermentation culture method,

[0070] a, preparation of slant spores: culture spore conditions: the slant spores are cultivated at a temperature of 38°C, the humidity is 60%, and the cultivation time is 120h; then the spores are placed under a deuterium lamp and irradiated for 60s under a 250-300nm ultraviolet light for modification, and the The modified spores are inoculated into the primary seed tank for cultivation;

[0071] b. Primary seed tank cultivation: the cu...

Embodiment 3

[0091] A kind of production technology of oxytetracycline sheet, specifically comprises the following steps:

[0092] (1) Preparation of fermentation broth;

[0093] Prepare oxytetracycline fermented liquid, select Streptomyces fistidis culture medium for use, the nitrogen source of described Streptomyces crackes culture medium is soybean cake powder, light yellow corn steep liquor, urea and egg white liquid, and carbon source is potato flour, dextrin ;Adopt purebred submerged, aeration and stirring three-stage fermentation culture method,

[0094] a, preparation of slant spores: culture spore conditions: slant spores are cultivated at a temperature of 36°C, humidity is 57%, and the incubation time is 100h; then the spores are placed under a deuterium lamp and irradiated for 50s under a 250-300nm ultraviolet light to modify, and the The modified spores are inoculated into the primary seed tank for cultivation;

[0095] b. Primary seed tank cultivation: the cultivation temper...

Images