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Cell structure, non-human model animal, manufacturing method of non-human model animal, and evaluation method of test substance

A technology of model animals and structures, applied in biochemical equipment and methods, cell culture support/coating, animal cells, etc., can solve the problem that gemcitabine does not show sufficient effectiveness, interstitial tissue does not grow sufficiently, and is not suitable for cancer Treatment of drugs and other issues to achieve an effective evaluation of the effect

Active Publication Date: 2021-07-06
FUJIFILM CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, especially in certain cancers such as pancreatic cancer, the stroma is abundant and fibrosis is prominent, so in the above-mentioned animal model, it can be seen that the stroma tissue does not grow sufficiently, and the state of the actual pancreatic cancer is very different from that of the actual pancreatic cancer. Results inappropriate for development of effective cancer treatments
In fact, it is known that gemcitabine developed as an effective therapeutic drug through the above-mentioned animal model does not show sufficient effectiveness in humans

Method used

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  • Cell structure, non-human model animal, manufacturing method of non-human model animal, and evaluation method of test substance
  • Cell structure, non-human model animal, manufacturing method of non-human model animal, and evaluation method of test substance
  • Cell structure, non-human model animal, manufacturing method of non-human model animal, and evaluation method of test substance

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0179] [Example 1] Recombinant peptide (recombinant gelatin)

[0180] The following CBE3 (described in International Publication WO2008 / 103041 A) was prepared as a recombinant peptide (recombinant gelatin).

[0181] CBE3:

[0182] Molecular weight: 51.6kD

[0183] Structure: GAP[(GXY) 63 ] 3 G

[0184] Number of amino acids: 571

[0185] RGD sequence: 12

[0186] Imino acid content: 33%

[0187] Approximately 100% of the amino acids are GXY repeat structures. The amino acid sequence of CBE3 does not contain serine, threonine, asparagine, tyrosine and cysteine. CBE3 has an ERGD sequence.

[0188] Isoelectric point: 9.34

[0189] GRAVY value: -0.682

[0190] 1 / IOB value: 0.323

[0191] Amino acid sequence (SEQ ID NO. 1 in Sequence Listing) (same as ID No. 3 of International Publication WO2008 / 103041. However, the X at the end is corrected to "P")

[0192] gaps

[0193] (GAPGLQGAPGLQGMPGERGAAGLPGPKGERGDAGPKGADGAPGAPGLQGMPGERGAAGLPGPKGERGDAGPKGADGAPGKDGVRGLAGPIGPPGER...

Embodiment 2

[0194] [Example 2] Production of recombinant peptide porous body

[0195] [PTFE thick / cylindrical container]

[0196] A cylindrical cup-shaped container made of polytetrafluoroethylene (PTFE) with a bottom thickness of 3 mm, a diameter of 51 mm, a side thickness of 8 mm, and a height of 25 mm was prepared. In the cylindrical cup, when the curved surface is used as the side surface, the side surface is sealed with 8mm PTFE, and the bottom surface (flat plate circle) is also sealed with 3mm PTFE. On the other hand, the top is in an open shape. Therefore, the inner diameter of the cylindrical cup becomes 43 mm. Hereinafter, this container is referred to as a PTFE thick / cylindrical container.

[0197] [Aluminum alloy glass plate / cylindrical container]

[0198] A cylindrical cup-shaped container made of aluminum alloy with a thickness of 1 mm and a diameter of 47 mm was prepared. In the cylindrical cup, when the curved surface is used as the side surface, the side surface is s...

Embodiment 3

[0224] [Example 3] Production of biocompatible polymer block (pulverization and crosslinking of porous body)

[0225]The CBE3 porous bodies of condition A and condition B obtained in Example 2 were pulverized using a new power mill (OSAKA CHEMICAL Co., Ltd., new power mill PM-2005). Regarding the pulverization, the pulverization was performed for a total of 5 minutes at the maximum rotation speed 1 minute x 5 times. The obtained pulverized product was sized by a stainless steel sieve to obtain uncrosslinked block materials of 25 to 53 μm, 53 to 106 μm, and 106 to 180 μm. Then, thermal cross-linking was performed at 160°C under reduced pressure (6 cross-linking times of 8 hours, 16 hours, 24 hours, 48 ​​hours, 72 hours, and 96 hours) to obtain a biocompatible polymer block (CBE3 block).

[0226] Hereinafter, the porous body-derived block under condition A crosslinked for 48 hours is referred to as E, and the porous body-derived block under condition B crosslinked for 48 hours...

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Abstract

The object of the present invention is to provide a non-human model animal having abundant mesenchymal tissue, a cell structure useful in the production of the above-mentioned non-human model animal, a method for evaluating a test substance using the above-mentioned non-human model animal, and A method for producing the above-mentioned non-human model animal. According to the present invention, there is provided a cell structure comprising a biocompatible polymer block and at least cancer cells and mesenchymal cells, wherein a plurality of the above-mentioned biocompatible Sexual polymer blocks.

Description

technical field [0001] The present invention relates to a cell structure useful in the production of non-human model animals for cancer diseases. The present invention also relates to a non-human model animal for cancer diseases and a production method thereof. The present invention also relates to a method for evaluating a test substance using the above-mentioned non-human model animal. Background technique [0002] Pancreatic cancer, which is one example of cancer, is an intractable cancer with an average survival period of 3 to 6 months. Various animal models are used in the development of drugs to treat cancers such as pancreatic cancer. For example, a tumor-bearing mouse model is used as a xenograft (Xenograft) model in which human pancreatic cancer cells are transplanted into immunodeficient mice. However, especially in certain cancers such as pancreatic cancer, the stroma is abundant and fibrosis is prominent, so in the above-mentioned animal model, it can be seen ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/15A61L27/00A61K35/13A61K35/28A61P35/00A61P37/04C12N5/077C12N5/09G01N33/50
CPCA61L27/00A61K35/13A61K35/28G01N33/50A01K67/0271A01K2207/12A01K2207/15A01K2207/30A01K2227/105A01K2267/0331A61P35/00A61P37/04A61P37/06A61P43/00A61K49/0008C12N5/0695C12N11/02C12N2533/54
Inventor 中村健太郎美马伸治北桥宗柿沼千早
Owner FUJIFILM CORP
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