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H1n1 flu virus neutralizing antibodies

An influenza virus and antibody technology, applied in antiviral agents, antiviral immunoglobulins, antibodies, etc., can solve problems such as poor results

Active Publication Date: 2018-04-17
MEDIGEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the vaccine is less effective in young children, older adults, and people with weakened immune systems

Method used

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  • H1n1 flu virus neutralizing antibodies
  • H1n1 flu virus neutralizing antibodies
  • H1n1 flu virus neutralizing antibodies

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0052] Cell culture and virus preparation

[0053] In RPMI1640 medium containing L-glutamine (Invitrogen, Carlsbad, CA, USA), 10% calf serum (FetalCalf Serum, FCS) and penicillin / streptomycin (Caisson) at 37 ° C and 5%CO 2 The marmoset B-lymphoblastoid B95-8 and 293T cells were cultured in a humidified environment. Bobtail hound kidney cells (Mardin-Darby canine kidney, MDCK) were subcultured in DMEM medium (Invitrogen) supplemented with 10% FCS. Spodoptera frugiperda (Sf9) cells were cultured in Sf-900II SFM medium (Invitrogen).

[0054] Influenza A H1N1 virus was prepared by Medigen Vaccine Biologics Co. (MVC, Taiwan). Influenza A virus A / Carlifornia / 7 / 2009NYMCX-179A (H1N1) is the proposed outbreak vaccine strain for vaccine development. Other clinical influenza A virus strains were obtained from Taiwan's Center for Disease Control (CDC) for the neutralization activity test described in this patent specification, 2013-80813 (A / Taiwan / 80813 / 2013(H1N1)), 2013-INF -152 (20...

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Abstract

An antibody, or a binding fragment of the antibody, against H1N1 virus, includes a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region contains complementarity determining regions (CDR) that have the amino acid sequences of SEQ ID NO: 5, SEQ ID NO: 6, and SEQ ID NO: 7; and wherein the light chain variable region contains complementarity determining regions that have the amino acid sequences of SEQ ID NO: 8, SEQ ID NO: 9, and SEQ ID NO: 10. A method for treating or preventing H1N1 infection in a subject includes administering to the subject theantibody or the binding fragment of the antibody.

Description

technical field [0001] The present invention relates to the field of using antibodies or fragments thereof for the treatment and prevention of influenza virus infection. Specifically, the present invention relates to the development of monoclonal antibodies for diagnosis, prevention and treatment of influenza A (H1N1) virus infection. Background technique [0002] When an infectious disease breaks out, finding effective treatment or prevention methods immediately is the key to controlling the spread of the disease. Recent influenza outbreaks, such as the 2009 H1N1 influenza outbreak, have highlighted the urgency of finding effective treatments. [0003] The outbreak of Influenza A (H1N1) in 2009 caused more than 60 million patients to be infected, and more than 2.5 million infected patients had to be hospitalized. The main epidemic virus in the 2009 H1N1 influenza outbreak, which the World Health Organization (WHO) called A(H1N1)pdm09, caused severe syndrome disproportiona...

Claims

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Application Information

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IPC IPC(8): C07K16/10C12N15/63C12N5/10G01N33/53A61K39/42A61P31/16
CPCA61K2039/505A61K2039/545A61P31/16C07K16/1018C07K2317/21C07K2317/76G01N33/56983G01N2333/11C07K2317/565
Inventor 林阳生赖癸太黄慧伦许湘婷陈宛贞陈雅琳翁嘉文
Owner MEDIGEN BIOTECH
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