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Application of a polypeptide in preventing or treating diseases related to cerebral ischemia-reperfusion injury

A technology for cerebral ischemia-reperfusion and disease, applied in the field of biomedicine, can solve the problems of lack of prevention and treatment drugs for cerebral ischemia-reperfusion injury, and achieve the goal of reducing cerebral ischemia-reperfusion injury, wide clinical application value, and significant protective effect Effect

Active Publication Date: 2020-12-25
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, there is still a lack of clear and effective drugs for the prevention and treatment of cerebral ischemia-reperfusion injury (see: "Research Progress in Traditional Chinese and Western Medicine Treatment of Cerebral Ischemia-Reperfusion Injury" (Tang Yibo et al., Journal of Cardiovascular and Cerebrovascular Diseases of Integrated Traditional Chinese and Western Medicine, pp. 2016, Vol. 14, No. 10, pp. 1100-1103); "Research Progress in Drug Therapy for Cerebral Ischemia-Reperfusion Injury" (Liang Chen et al., China Pharmacy, 2016, Vol. 27, No. 4, pp. 538-541) ), therefore, the development of drugs that can reduce cerebral ischemia-reperfusion injury is of great significance for the prevention and treatment of ischemic cerebrovascular diseases

Method used

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  • Application of a polypeptide in preventing or treating diseases related to cerebral ischemia-reperfusion injury
  • Application of a polypeptide in preventing or treating diseases related to cerebral ischemia-reperfusion injury
  • Application of a polypeptide in preventing or treating diseases related to cerebral ischemia-reperfusion injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Example 1 The protective effect of NKI9 on cerebral ischemia-reperfusion injury

[0011] 1 Experimental materials

[0012] C57BL / 6J mice were purchased from Beijing Huafukang Biotechnology Co., Ltd. [Experimental animal certificate: SCXK (Beijing) 2014-0004; SYXK (Guangdong) 2015-0147]; low molecular weight heparin calcium was purchased from Guangdong Tianpu Biochemical Pharmaceutical Co., Ltd. company's product.

[0013] NKI9 and its mutants are obtained by the inventors through prokaryotic expression system recombinant preparation, and the purity is greater than 95%. Specifically, the nucleic acid sequence encoding the target polypeptide (such as NKI9) was ligated into the prokaryotic expression vector pET32a-sumo, and sequencing showed that the correct recombinant plasmid was transferred into Escherichia coli BL21(DE3), induced by IPTG, and the host cells were isolated 1. After sonication, the expression product was purified by nickel affinity chromatography to obt...

Embodiment 2

[0033] Example 2 Effect of NKI9 on mouse tail bleeding time (BT)

[0034] 1 Experimental materials

[0035] Recombinant NKI9 (rNKI9) and its mutants were recombined and prepared by the inventor in the laboratory, with a purity greater than 95%, and the recombination preparation method was the same as in Example 1; Balb / c mouse experimental animal certificate: SCXK (Guangdong) 2013-0008; SYXK (Guangdong) 2015-0147, low molecular weight heparin calcium is a product of Guangdong Tianpu Biochemical Pharmaceutical Co., Ltd.

[0036] 2 Experimental methods

[0037]2.1 Animal grouping: 65 Balb / c mice weighing 18-22 g, half male and half male, were randomly divided into 13 groups, 5 mice in each group, respectively: blank control group (normal saline group), low molecular weight heparin calcium group ( 375U / kg), recombinant NKI9 and its mutant NKI9-1~NKI9-10 group (1.0mg / kg).

[0038] 2.2 Administration and measurement of bleeding time: intraperitoneal injection of 2% pentobarbital...

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PUM

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Abstract

The invention discloses the application of polypeptide to prevention or treatment of cerebral ischemia-reperfusion injury related diseases, and belongs to the field of biomedicines. The amino acid sequence of the polypeptide is as follows: SEQ ID NO.1 or SEQ ID NO.2 or SEQ ID NO.3 or SEQ ID NO.4 or SEQ ID NO.5 or SEQ ID NO.6 or SEQ ID NO.7 or SEQ ID NO.8 or SEQ ID NO.9 or SEQ ID NO.10 or SEQ ID NO.11. The polypeptide can significantly reduce cerebral ischemia-reperfusion injury, can be used as a medicine for preventing or treating the cerebral ischemia-reperfusion injury related diseases, andhas a clinical application value.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular, the invention relates to the application of a polypeptide in preventing or treating diseases related to cerebral ischemia-reperfusion injury. Background technique [0002] Ischemic cerebrovascular disease is a common clinical cerebrovascular disease, which has the characteristics of high morbidity, high mortality, and high disability rate, accounting for about 70% to 80% of all cerebrovascular patients. Ischemic stroke is currently the second leading cause of human death. About 30% of stroke patients in my country died, and 70% of the survivors suffered from aphasia, hemiplegia and other disabilities, seriously affecting people's health and quality of life. At present, it is still the primary clinical treatment principle to restore or recirculate the ischemic brain tissue as soon as possible, and to regain blood oxygen supply. Aggravated, the clinical symptoms worsened, that is to caus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/16A61P9/10
CPCA61K38/16
Inventor 彭礼飞罗辉邵正司徒永立邓莉廖淑莉何庆丰
Owner GUANGDONG MEDICAL UNIV
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