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Glioma prognostic marker hsa_circ_0125365 and application

A prognostic marker and glioma technology, applied in biochemical equipment and methods, microbiological measurement/testing, DNA/RNA fragments, etc., can solve the problem of poor survival rate and no obvious improvement in the survival rate of glioma patients, etc. question

Active Publication Date: 2018-04-20
XIANGYA HOSPITAL CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not been significantly improved.
The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information, and once diagnosed, most of them are in the middle and advanced stages, and the survival rate after surgery is not optimistic

Method used

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  • Glioma prognostic marker hsa_circ_0125365 and application
  • Glioma prognostic marker hsa_circ_0125365 and application
  • Glioma prognostic marker hsa_circ_0125365 and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Preparation of reagents for detecting the expression level of circRNA hsa_circ_0125365 for the preparation of a kit for the prognosis of glioma patients (50 reactions)

[0019] 1. RNA stabilization solution 50ml

[0020] 2. Isopropanol 100ml

[0021] 3. Chloroform 100ml

[0022] 4. Trizol (from Molecular Research Center company) 50ml

[0023] 5. Enzyme-free water 10ml

[0024] 6.1 μM random reverse transcription primer (Thermo Company) 50 μl

[0025] 7.5 × reverse transcription buffer (Thermo company) 200ml

[0026] 8. 10mM base triphosphate deoxynucleotide (Thermo company) 100μl

[0027] 9.40U / μl RNase inhibitor (Thermo company) 500μl

[0028] 10.200U / μl MMLV reverse transcriptase (Thermo Company) 50μl

[0029] 11. Premix Ex Taq (Thermo Company) 50μl

[0030] 12.10μM circRNA hsa_circ_0125365 real-time fluorescence quantitative PCR specific primer 30μl

[0031] circRNA hsa_circ_0125365 forward primer: 5'-CCAAGCAGCTCACTACGATA-3',

[0032] circRNA hsa...

Embodiment 2

[0036] Example 2: The relationship between the expression level of circRNA hsa_circ_0125365 in glioma tissue and prognosis

[0037] 1. Preservation of glioma tissue: collect the glioma tissue to be tested and store it in a cryopreservation tube filled with RNA stabilization solution, and put it in a -80°C refrigerator for later use.

[0038] 2. Extraction of RNA in tissues: Take an appropriate amount of specimen, add liquid nitrogen to the mortar after baking at 180°C for 6-8 hours, grind the specimen, grind to powder, add 1ml Trizol mortar specimen to the mortar, and grind into After liquid, move to tube, add 200μl of chloroform to the tube, shake by hand for 15-30s, place on ice for 15min, centrifuge at 12000rpm at 4°C for 15min; carefully take the upper aqueous phase into a new tube, add pre-cooled isopropanol Mix with 0.5ml, keep it on ice for 20min, centrifuge at 12000rPm at 4°C for 10min; discard the supernatant, add 1-2ml of ethanol diluted with 75% DEPC water, mix even...

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Abstract

The invention belongs to the biotechnology field, and discloses a glioma prognostic marker hsa_circ_0125365 and application. A reagent for detecting circRNA hsa_circ_0125365 of a glioma source is usedfor preparing a prognostic preparation of a glioma patient. Research proves that the glioma patient with relatively high expression of circRNA hsa_circ_0125365 has relatively high postoperative survival rate. A prognosis of the glioma patient is made by detecting the expression level of circRNA hsa_circ_0125365 in glioma tissue of the glioma patient.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a circRNA marker for glioma prognosis, the application of a reagent for detecting the marker to prepare a glioma prognosis preparation, and a kit. Background technique [0002] Glioma is the most frequent brain tumor in adults, accounting for 40.49% of intracranial tumors. From the time of diagnosis, the average life expectancy of patients with glioma is less than five years. In order to diagnose and treat glioma, a malignant disease highly correlated with genetic material, it is necessary to explore its pathogenesis at the level of molecular biology and from the expression of genetic information. At present, although the diagnosis and treatment methods of glioma are in the stage of continuous improvement, the survival rate of glioma patients has not improved significantly. The diagnosis of glioma is still in the empirical stage based on clinical, pathological and imaging information...

Claims

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/118C12Q2600/158C12Q2600/178
Inventor 武明花冯紫阳李沛瑶刘长红刘涛佘晓玲孙英男
Owner XIANGYA HOSPITAL CENT SOUTH UNIV
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