Preparation method of biological active protein material for cigarette filter tip and filter tip
A technology of biologically active protein and biological protein, which is applied in the field of preparation of cigarette filters and filter materials, and biologically active protein materials, and can solve problems such as refractory degradation of cellulose acetate components, environmental hazards, and sensitive market reactions
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[0031] A method for preparing a bioactive protein material for cigarette filters, comprising the following steps:
[0032] (1) Protein gene fusion
[0033] Centrifuge fresh blood at 10°C at a speed of 500r / min for 2 minutes, then centrifuge at a speed of 2000r / min for 10-12 minutes, take the third layer of liquid, namely the hemoglobin solution; send the separated hemoglobin solution into the Bacteria laboratory, use sequence analyzer, peptide synthesizer and amino acid sequence analyzer to separate α chain and protein β chain, and synthesize biological protein through cell fusion instrument;
[0034] (2) Protein culture
[0035] Send the fused biological protein into the protein tank, add distilled water twice the mass of the biological protein into the protein tank for cultivation, the cultivation temperature is 30°C, the air volume is 0.8 times the volume of the protein tank, cultivate for 2 hours, and then add to the sedimentation tank Tryptone soybean agar medium with 8...
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