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Preparation method of biological active protein material for cigarette filter tip and filter tip

A technology of biologically active protein and biological protein, which is applied in the field of preparation of cigarette filters and filter materials, and biologically active protein materials, and can solve problems such as refractory degradation of cellulose acetate components, environmental hazards, and sensitive market reactions

Active Publication Date: 2018-04-24
徐智辉
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the commonly used technical means are: in the ordinary filter material, a single addition of porous and high surface area activated carbon, silicate (activated carbon has a low adsorption capacity for polar substances and carbon monoxide), or biologically active extracts ( More complicated, unpredictable reaction with smoke)
These types of substances differ in the selectivity, efficiency, complexity, and cost control of adsorbing harmful substances. Smoking taste, which is particularly important in the domestic market mainly dominated by flue-cured tobacco, the market reaction is very sensitive in this respect
In addition, the acetate fiber component of ordinary filters is difficult to degrade, causing certain harm to the environment
In short, as the governments of various countries impose stricter restrictions on cigarettes, and consumers pay more and more attention to smoking and health, as well as the demand for smoking taste, it is difficult to meet the needs of the cigarette market only with a special filter with a single function additive. Development needs, that is to say, the special filter has developed from a single function of an additive to a multi-functional direction of multiple additives

Method used

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  • Preparation method of biological active protein material for cigarette filter tip and filter tip
  • Preparation method of biological active protein material for cigarette filter tip and filter tip
  • Preparation method of biological active protein material for cigarette filter tip and filter tip

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specific Embodiment

[0031] A method for preparing a bioactive protein material for cigarette filters, comprising the following steps:

[0032] (1) Protein gene fusion

[0033] Centrifuge fresh blood at 10°C at a speed of 500r / min for 2 minutes, then centrifuge at a speed of 2000r / min for 10-12 minutes, take the third layer of liquid, namely the hemoglobin solution; send the separated hemoglobin solution into the Bacteria laboratory, use sequence analyzer, peptide synthesizer and amino acid sequence analyzer to separate α chain and protein β chain, and synthesize biological protein through cell fusion instrument;

[0034] (2) Protein culture

[0035] Send the fused biological protein into the protein tank, add distilled water twice the mass of the biological protein into the protein tank for cultivation, the cultivation temperature is 30°C, the air volume is 0.8 times the volume of the protein tank, cultivate for 2 hours, and then add to the sedimentation tank Tryptone soybean agar medium with 8...

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Abstract

The invention discloses a preparation method of a biological active protein material for a cigarette filter tip and a filter tip structure combining the biological active protein material. The preparation method of the invention comprises the following steps: fusing protein genes; then sending the fused biological proteins into a protein trough, adding distilled water which is two times of the quantity of the biological proteins into the protein trough, performing the culture, wherein the culture temperature is 30 DEG C, introducing air which is 0.8 time of the volume of the protein trough, culturing for 2h, and then adding a tryptone soy agar culture medium which is 80 times of the mass of the biological proteins into a settling pond; adding active carbon with a particle size of 40 meshesto 60 meshes into the settling pond, keeping the temperature at 10 DEG C, and stirring for 20 minutes at the speed of 90 rpm, enabling the active carbon to be sufficiently fused with the proteins, and after the chelating reaction is sufficiently completed, collecting supernatant; and finally coating the carrier to obtain active carbon particles with a protein titer of 2 billion / g or more. The preparation method has the advantages that the harmful substance removal efficiency is high, and the immunity of a smoker can be basically prevented from being harmed.

Description

technical field [0001] The invention relates to a cigarette filter and its filter material, in particular to a method for preparing a biologically active protein material used in a cigarette filter, and a filter suitable for the biologically active protein material. Background technique [0002] It has been officially confirmed that there are at least 5,289 components in tobacco and cigarette smoke, and 149 harmful components. At present, the global medical community and the tobacco industry have unanimously recognized and concerned about 46 of them. Tobacco factories around the world are developing and innovating on the topic of harm reduction and coke reduction, but so far there is no particularly ideal technology. With the increasing anti-tobacco movement in the world and the general increase of consumers' attention to smoking and health, the market has higher and higher requirements for cigarettes, and the tobacco industry is facing more and more pressure, especially in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K17/14A24D3/04A24D3/10A24D3/08A24D3/16
CPCA24D3/04A24D3/08A24D3/10A24D3/16C07K17/14
Inventor 徐智辉
Owner 徐智辉