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Tissue culture method of lagerstroemia venusta wall. ex clarke.

A technology of tissue culture and crape myrtle, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate and achieve the effects of easy reproduction, shortened reproduction cycle, and increased survival rate

Inactive Publication Date: 2018-05-04
合肥欣和园苗木有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] When Xishuangziwei is transplanted to central China, the ornamental effect is very good, but the survival rate is very low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A method for tissue culture of Lagerstroemia chinensis, comprising the following steps:

[0021] 1) Disinfection and sterilization of inoculated branches: select the tender branches of Lagerstroemia chinensis in the same year, soak the young branches in bleaching powder for 5 minutes, rinse them in running water for 20 minutes, soak them in 75% alcohol on the ultra-clean workbench for 2 minutes, take them out and use Rinse 5 times with sterile water prepared in advance, drain the water, and inoculate the sterilized twigs on the germination medium;

[0022] 2) Inoculation culture: the selected explants were cut into 1 cm segments and inoculated on the prepared inoculation medium, and cultivated for 25 days;

[0023] 3) Subculture: Inoculate the excised clustered buds into the subculture medium for cultivation, at a temperature of 23-27° C., with a light intensity of 2500 lux, and a daily light of 10 hours. After 30 days, calluses are formed at the base of the small buds,...

Embodiment 2

[0031] A method for tissue culture of Lagerstroemia chinensis, comprising the following steps:

[0032] 1) Disinfection and sterilization of inoculated branches: select the tender branches of Lagerstroemia chinensis in the same year, soak the young branches in bleaching powder for 10 minutes, rinse them in running water for 30 minutes, soak them in 75% alcohol on the ultra-clean workbench for 3 minutes, take them out and use Rinse 4 times with pre-prepared sterile water, drain the water, and inoculate the sterilized twigs on the bud-inducing medium;

[0033] 2) Inoculation culture: the selected explants were cut into 1-2 cm segments and inoculated on the prepared inoculation medium, and cultivated for 30 days;

[0034] 3) Subculture: Inoculate the excised clustered buds into the subculture medium for cultivation, at a temperature of 23-27° C., with a light intensity of 2200 lux, and a daily light of 12 hours. After 32 days, calluses are formed at the base of the small buds, an...

Embodiment 3

[0042] A method for tissue culture of Lagerstroemia chinensis, comprising the following steps:

[0043] 1) Disinfection and sterilization of inoculated branches: select the tender branches of Lagerstroemia chinensis in the same year, soak the young branches in bleaching powder for 8 minutes, rinse them in running water for 25 minutes, soak them in 75% alcohol on the ultra-clean workbench for 3 minutes, take them out and use Rinse 5 times with pre-prepared sterile water, and inoculate the shoots after draining the water on the bud-inducing medium;

[0044]2) Inoculation culture: the selected explants were cut into 1-2 cm segments and inoculated on the prepared inoculation medium, and cultivated for 30 days;

[0045] 3) Subculture: inoculate the excised clustered buds into the subculture medium for cultivation, at a temperature of 23-27°C, with a light intensity of 2800 lux, and 10 hours of light per day, until 28 days, callus tissue is formed at the base of the small buds, and ...

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PUM

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Abstract

The invention provides a tissue culture method of lagerstroemia venusta wall. ex clarke. The tissue culture method comprises the following steps: (1) sterilizing inoculated branches; (2) carrying outinoculation culture: cutting selected explants into small pieces of 1-2 cm, then inoculating the cut explant pieces on a prepared inoculation medium and culturing for 25-30 days; (3) carrying out subculture: inoculating cut cluster buds on a subculture medium, and culturing for 28-32 days until calluses and cluster buds are formed at the roots of budlet and the cluster budlets are as tall as 1-2cmunder the conditions that the temperature is 23-27 DEG C, the illumination intensity is 2200-2800 lux and daily illumination time is 10-12 hours; (4) carrying out rooting culture: when the seedlingsgrow to 2-3cm, transferring the seedlings to a rooting medium, and culturing for 20-25 days under the conditions that the temperature is controlled to be 25-28 DEG C, the light intensity is 1200-1500lux, and the daily illumination time is 10-12 hours; (5) exercising seedlings and transplanting. The tissue culture method breaks the limit of seasons and climate, shortens the breeding cycle and improves the survival rate of regenerated plants, and the survival rate can reach 95% or above.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a method for tissue culture of Lagerstroemia chinensis. Background technique [0002] Lagerstroemia venusta Wall.ex Clarke, Latin name: Lagerstroemia venusta Wall.ex Clarke. Lagerstroemia venusta Wall.ex Clarke. Arbor of the genus Lagerstroemiaceae, up to 8 meters high; branches glabrous or gray-white pilose, gray-brown when dry. Leaves opposite or nearly opposite, papery, oblong or obround-elliptic, apex obtuse, sparsely subrounded, base subrounded, often unequal on both sides, glabrous on both sides, or glabrous when young There are fine hairs on the lower veins, the stamens are numerous, and the rest are nearly equal; the ovary is glabrous, spherical, the style is long and slender, and the stigma is capitate. The capsule is nearly spherical to obovate, and the persistent calyx is cup-shaped; the flowering period is October, and the fruiting period is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王国来
Owner 合肥欣和园苗木有限公司