Microorganism nutrient agent for anaerobic digested sludge bioleaching treatment as well as preparation method and application of microorganism nutrient agent

A microbial nutrition and anaerobic sludge technology, which is applied in the field of microbial nutrient and its preparation for anaerobic digested sludge bioleaching treatment, can solve the problem that cannot meet the needs of anaerobic digested sludge growth and cannot meet the growth of complex microorganisms Demand, bioleaching process can not be effectively dealt with and other problems, to achieve the effect of improving microbial activity, low price, and improving mutual inhibition

Active Publication Date: 2018-05-04
南京贝克特环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Anaerobic digested sludge and municipal domestic sludge have great differences in sludge properties. Anaerobic digested sludge contains small molecular organic acids, such as formic acid, acetic acid, propionic acid, butyric acid, etc., and contains suspended solids, ammonia nitrogen, phosphorus, etc. and extracellular polymers, so the use of special microorganisms and nutrients for bioleaching of municipal sludge cannot meet the needs of microbial growth in anaerobic digested sludge bioleaching. The types and quantities are closely related. The microbial nutrients used in the two kinds of sludge bioleaching are completely different. The Chinese patent application number is 201410119833. A new method of rapid conditioning and deep dehydration by bioleaching. The bioleaching compound microorganisms used in this patent are composed of acidophilic Thiobacillus (Thiobacillus ferrooxidans LX5 and Thiobacillus thiooxidans TS6), Pichia pastoris D13, Rhodotorula R30, Geotrichum Z3 is suitable for undigested ordinary sludge. The specific composition of microbial nutrients used can be found in ZL201010221264.1. Due to the specificity of anaerobic digestion sludge, traditional bioleaching technology cannot effectively and stably treat it
Bioleaching technology generally uses complex microbial flora as working bacteria. Due to the different types and growth conditions of various microorganisms in the complex microbial flora, simply mixing nutrients suitable for the growth of each microorganism cannot meet the requirements. The growth requirements of the overall complex microorganisms may even inhibit the normal growth and reproduction of some microorganisms

Method used

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  • Microorganism nutrient agent for anaerobic digested sludge bioleaching treatment as well as preparation method and application of microorganism nutrient agent
  • Microorganism nutrient agent for anaerobic digested sludge bioleaching treatment as well as preparation method and application of microorganism nutrient agent
  • Microorganism nutrient agent for anaerobic digested sludge bioleaching treatment as well as preparation method and application of microorganism nutrient agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The process steps of producing the microbial nutrient for anaerobic digested sludge bioleaching are as follows:

[0032] (1) Obtain complex microbial flora

[0033] Inoculate Thiobacillus ferrooxidans LX5 (A.ferrooxidans LX5, preservation number CGMCC NO.0727) and Bordetella ZW2 (Bordetella sp. preservation number CGMCC NO.3033) isolated from the sludge into their respective cultures. base, and then placed at 25°C under aerated conditions (3m 3 / (h·m 2 )) Propagation and cultivation until the number of bacterial cells reaches 10 8 individual / mL. Mix the culture solution of Thiobacillus ferrooxidans LX5 and Bordetella ZW2 at a volume ratio of 3:1 to obtain a composite microbial bacterial solution. The total volume of the composite microbial bacterial liquid is 5L.

[0034] (2) Fermentation of nutrient compound flora

[0035] Weigh 50g of sucrose, 10g of peptone, 10g of potassium dihydrogen phosphate, 40g of ammonium chloride, 10g of ammonium hydrogen phosphate, 50g...

Embodiment 2

[0052] The technological steps of producing the microbial nutrient agent of above-mentioned 1kg anaerobic digested sludge bioleaching process are as follows:

[0053] (1) Obtain complex microbial flora

[0054] With embodiment 1.

[0055] (2) Fermentation of nutrient compound flora

[0056] Weigh 100g of sucrose, 30g of peptone, 20g of potassium dihydrogen phosphate, 60g of ammonium chloride, 30g of ammonium hydrogen phosphate, 80g of ammonium sulfate, 20g of magnesium sulfate heptahydrate, 80g of sodium thiosulfate pentahydrate, 20g of calcium nitrate, 100g of iron sulfate, Manganese sulfate 30g, zinc sulfate 60g, potassium chloride 30g, ferrous sulfate heptahydrate 100g, ammonium sulfate 100g, sulfur 200g.

[0057] Dissolve the above-mentioned nutrients in 10L of water, add the complex microbial bacterial liquid, and under the condition of 23°C and aeration (5m 3 / (h·m 2 )) Cultivate until the pH of the culture system drops to 3.0 and stop the culture.

[0058] (3) The ...

Embodiment 3

[0068] The process steps of producing the microbial nutrient for anaerobic digested sludge bioleaching are as follows:

[0069] (1) Obtain complex microbial flora

[0070] With embodiment 1.

[0071] (2) Fermentation of nutrient compound flora

[0072] Weigh 200g of sucrose, 50g of peptone, 30g of potassium dihydrogen phosphate, 100g of ammonium chloride, 50g of ammonium hydrogen phosphate, 100g of ammonium sulfate, 30g of magnesium sulfate heptahydrate, 80g of sodium thiosulfate pentahydrate, 30g of calcium nitrate, 100g of iron sulfate, Manganese sulfate 50g, zinc sulfate 100g, potassium chloride 40g, ferrous sulfate heptahydrate 50g, ammonium sulfate 100g, sulfur 100g.

[0073] Dissolve the above-mentioned nutrients in 10L of water, add the complex microbial bacterial liquid, and under the condition of 28°C and aeration (6m 3 / (h·m 2 )) Cultivate until the pH of the culture system drops to 3.0 and stop the culture.

[0074] (3) The process steps of solid-liquid separatio...

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Abstract

The invention discloses a microorganism nutrient agent for anaerobic digested sludge bioleaching treatment as well as a preparation method and application of the microorganism nutrient agent and belongs to the technical field of sludge treatment. According to the microorganism nutrient agent, high-nutrition substances are adopted as raw materials, a solid substance is prepared through fermentationand filter pressing dehydration of microorganisms (ferrous oxide thiobacillus LX5 and bordetella ZW2), and the content of microbial colonies in the microorganism nutrient agent is 10<7>-10<8> pieces / g. The preparation method of the microorganism nutrient agent comprises the following steps: (1) performing composite microorganism culture; (2) performing nutrient substance composite colony fermentation; (3) performing solid-liquid separation; (4) mixing auxiliary materials; (5) performing sub-packaging. By adopting the microorganism nutrient agent, nutrient components can meet requirements of growth of anaerobic digested sludge bioleaching composite microorganism biological colonies, and stable operation of a bioleaching system is ensured. The microorganism nutrient agent has the advantagesof being low in price, easy to obtain and free of pollution, is simple and convenient in production process and is an environmental-friendly nutrient agent.

Description

technical field [0001] The invention belongs to the technical field of sludge treatment, and more specifically relates to a microbial nutrient used for bioleaching treatment of anaerobic digested sludge, a preparation method and application thereof. Background technique [0002] In recent years, with the acceleration of my country's urbanization process and the improvement of environmental quality standards, the amount of sewage to be treated has increased sharply. The principle of sewage treatment is to enrich and transform organic matter and inorganic nitrogen, phosphorus, etc. in sewage through mechanical action and microbial metabolism, and then produce another pollutant (sludge). At present, my country's sludge production is huge, and at an annual rate of 10 The speed of %-15% shows an increasing trend. [0003] Compared with traditional sludge conditioning methods, bioleaching technology can not only remove heavy metals in sludge, but also remove most pathogenic bacter...

Claims

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Application Information

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Patent Type & AuthorityApplications(China)
IPC IPC(8): C12N1/20C12N1/16C02F11/04C12R1/01C12R1/84
CPCC02F11/04C12N1/16C12N1/20
Inventor周立祥李浙英胡洪彬
Owner南京贝克特环保科技有限公司