Eel soy sauce and preparation method thereof
A production method and eel technology, applied in the direction of food science, etc., can solve problems such as limiting the application of eel enzymatic hydrolyzate, affecting product processing and preservation, and prominent fishy smell of eel meat, so as to improve enzymatic hydrolysis efficiency and production yield, production Low cost, clear effect of fish flavor
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Embodiment 1
[0042] A preparation method of eel soy sauce, comprising the following steps:
[0043] (1) Remove the viscera of fresh or chilled eel, wash it with clean water, drain it, and pass it through a meat grinder to obtain minced eel; add minced eel and its mass 8% 98% ethanol solution and 3.0 % glucose, stir evenly; cover and seal and heat up to 90°C, then keep warm and stir for 20min to carry out the Maillard reaction; after the reaction, cool down to about 60°C to obtain the eel Maillard reaction product;
[0044] (2) Add eel Maillard reaction product and water with 1.5 times its mass to the enzymatic hydrolysis irrigation, raise the temperature to 50°C, add Alcalase 2.4L (Novozymes) with 0.3% mass of eel Maillard reaction product and 0.8% Flavourzyme 500MG (Novozymes) was hydrolyzed at 50°C for 4 hours, and after the enzymolysis was completed, it was incubated at 90°C for 20min to inactivate the enzyme, and centrifuged (5000×g, 30min), the upper layer of oil and The lower sedime...
Embodiment 2
[0049] A preparation method of eel soy sauce, comprising the following steps:
[0050] (1) Remove the viscera of fresh or chilled eel, wash it with clean water, drain it, and pass it through a meat grinder to obtain minced eel; add minced eel and its mass 12% 98% ethanol solution and 1.0 % ribose, stir evenly; cover and seal and heat up to 80°C, then keep warm and stir for 40min to carry out the Maillard reaction; after the reaction, cool down to about 60°C to obtain the eel Maillard reaction product;
[0051] (2) Add the eel Maillard reaction product and 0.5 times its mass of water to the enzymatic hydrolysis irrigation, heat up to 60°C, add Alcalase 2.4L (Novozymes) with a mass of 0.7% of the eel Maillard reaction product and 1.2% Flavourzyme 500MG (Novozymes) was hydrolyzed at 60°C for 3 hours. After the enzymolysis, it was incubated at 85°C for 30min to inactivate the enzyme. Centrifuge (5000×g, 30min) to discard the upper layer of oil and The lower sediment, the supernat...
Embodiment 3
[0056] A preparation method of eel soy sauce, comprising the following steps:
[0057] (1) Remove the viscera of fresh or chilled eel, wash it with clean water, drain it, and pass it through a meat grinder to obtain minced eel; add minced eel and its 10% 98% ethanol solution of mass and 2.0 to the enzymolysis tank % xylose, stir evenly; cover and seal and heat up to 88°C, then keep warm and stir for 25min to carry out the Maillard reaction; after the reaction, cool down to about 60°C to obtain the eel Maillard reaction product;
[0058] (2) Add eel Maillard reaction product and water with 1.0 times its mass to the enzymatic hydrolysis irrigation, heat up to 55°C, add Alcalase 2.4L (Novozymes) with 0.5% mass of eel Maillard reaction product and 1.0% Flavourzyme 500MG (Novozymes) was hydrolyzed at 55°C for 3.5 hours. After the enzymolysis, it was incubated at 88°C for 25min to inactivate the enzyme, and centrifuged (5000×g, 30min) was used to discard the upper layer of oil and ...
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