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Uses of 1,3,4-tri-o-(e)-caffeoyl-β-d-glucopyranose

A technology of caffeoyl and pyranose, which is applied in the field of preparation of hypoglycemic drugs or food, and can solve the problems that there is no anti-α-glucosidase biological activity.

Inactive Publication Date: 2020-02-21
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

"At present, there is no report on the biological activity of this compound against α-glucosidase

Method used

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  • Uses of 1,3,4-tri-o-(e)-caffeoyl-β-d-glucopyranose
  • Uses of 1,3,4-tri-o-(e)-caffeoyl-β-d-glucopyranose
  • Uses of 1,3,4-tri-o-(e)-caffeoyl-β-d-glucopyranose

Examples

Experimental program
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Effect test

Embodiment 1

[0011] The 1,3,4-tri-O-(E)-caffeoyl-β-D-glucopyranose represented by formula I was prepared according to Example 1 of Chinese Patent ZL201310134172.3.

[0012] Example 1

[0013] The present invention is to take the dry root tuber of pseudocihobic as raw material, pulverize, use 2.5 times of the volume concentration of the ethanol aqueous solution of 95%, 95% and 60% successively to reflux extract 3 times, each time 2 hours, filter, combine the extracts, Recover under reduced pressure until there is no alcohol smell, disperse the residue in distilled water to form an aqueous suspension with a volume concentration of 40%, extract with petroleum ether and ethyl acetate in turn, and recover the extracted part of ethyl acetate to dryness under reduced pressure. Several times of silica gel column chromatography and polyamide column chromatography, two phenylpropanoid compounds were obtained, and the structure of the compound was determined by nuclear magnetic resonance spectroscopy...

Embodiment 2

[0021] Determination of α-glucosidase inhibitory activity

[0022] Preparation of reaction solution

[0023] Prepare phosphate buffer: weigh 1.167g of dipotassium hydrogen phosphate and 0.9118 of potassium dihydrogen phosphate, add an appropriate amount of distilled water to dissolve, then use a volumetric flask to set the volume to 100mL, and prepare a phosphate buffer with pH=6.8 for later use.

[0024] Preparation of substrate PNPG (4-nitrophenyl-α-D-glucopyranoside) solution: Weigh 90.375mg PNPG, add appropriate amount of phosphate buffer (pH=6.8) to dissolve, and then use a volumetric flask to accurately dilute to 25mL, prepared as a 12mmol / L mother solution. Dilute the mother liquor to a 4mmol / L solution for subsequent use.

[0025] Preparation of α-glucosidase enzyme solution: dissolve freeze-dried enzyme powder (enzyme activity 14u / mg) in phosphate buffer (pH=6.8) to prepare a 0.15u / mL mother solution for later use.

[0026] Prepare Na 2 CO 3 Solution: weigh 2.12g...

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Abstract

The invention discloses use of 1,3,4-tris-O-(E)-caffeoyl-beta-D-glucopyranose for preparation of hypoglycemic agents or foods. The 1,3,4-tris-O-(E)-caffeoyl-beta-D-glucopyranose is shown in a formula(I). Experiments show that the 1,3,4-tris-O-(E)-caffeoyl-beta-D-glucopyranose is far superior to acarbose in inhibiting alpha-glucosidase activity, and the 1,3,4-tris-O-(E)-caffeoyl-beta-D-glucopyranose can be used for preparation of antidiabetic foods and pharmaceuticals.

Description

technical field [0001] The invention relates to the use of extracting 1,3,4-tri-O-(E)-caffeoyl-β-D-glucopyranose from plants in preparing hypoglycemic drugs or food. Background technique [0002] False cohosh (Aruncus sylvester Kostel.), belongs to Rosaceae (Rosaceae) Spiraeoideae subfamily (Spiraeoideae Agardh) and pseudo cohosh (Aruncus Adans.). The dried root of False cohosh is called "Golden Hair Sanqi", which is often used as a traditional Chinese medicine to treat muscle strain, and has the effect of relaxing tendons and activating collaterals. [0003] Chinese patent ZL201310134172.3 discloses antioxidant active compounds and their uses, one of which is 1,3,4-tri-O-(E)-caffeoyl-β-D-glucopyranose, and discloses Its "application in the preparation of antioxidant drugs, food or cosmetics." At present, there is no report on the biological activity of this compound against α-glucosidase. Contents of the invention [0004] The purpose of the present invention is to prov...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/7024A61P3/10A23L33/125A23L33/105
CPCA23L33/105A23L33/125A23V2002/00A61K31/7024A23V2200/328A23V2250/21
Inventor 苏艳芳栗章彭魏子烨
Owner TIANJIN UNIV