Microwave sensitization composite nano particles as well as preparation method and application thereof

A composite nanoparticle and microwave sensitization technology, which is applied in the direction of wave energy or particle radiation treatment materials, microcapsules, nanocapsules, etc., to prolong the recurrence time, reduce the progression rate, and improve the anti-tumor effect

Active Publication Date: 2018-05-08
GENERAL HOSPITAL OF PLA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no method to specifically improve the sensitivity of tumor cells to microwave action by using the pathophysiological differences between tumor cells and normal tissue cells, that is, to achieve the killing effect on tumor cells under non-lethal temperature conditions

Method used

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  • Microwave sensitization composite nano particles as well as preparation method and application thereof
  • Microwave sensitization composite nano particles as well as preparation method and application thereof
  • Microwave sensitization composite nano particles as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Weigh 200mg mPEG-PLGA and dissolve it in 4mL of dichloromethane (DCM), and add it to 0.5mL of the first aqueous solution in which doxorubicin (DOX), NaCl and polyvinyl alcohol (PVA) are dissolved, (PVA The mass concentration is 0.5%, the mass concentration of NaCl is 30mg / mL, and the mass concentration of DOX is 10mg / mL), emulsified in an ice bath at -10~-5°C for 90s to obtain an emulsion, and the ultrasonic power is 180W;

[0035] (2) Add 10 mL of the second aqueous solution with 5 wt% PVA dissolved in the emulsion obtained in step (1), emulsify in an ice bath at -10 to -5°C for 90 seconds, and use an ultrasonic power of 400W;

[0036] (3) Transfer the emulsion obtained in step (2) to 40 mL of a third aqueous solution in which 0.1 wt % PVA is dissolved, and magnetically stir for 4 h to remove dichloromethane (DCM);

[0037] (4) Centrifuge at 12000r / min for 40min to collect the precipitate, wash with deionized water three times to obtain NaCl+DOX@PLGA nanoparticles....

Embodiment 2

[0040] (1) Weigh 200mg mPEG-PLGA and dissolve it in 6mL of dichloromethane (DCM), add it to 1mL of the first aqueous solution in which doxorubicin (DOX), NaCl and polyvinyl alcohol (PVA) are dissolved, (PVA mass The concentration is 1%, the mass concentration of NaCl is 30mg / mL, and the mass concentration of DOX is 10mg / mL), emulsified in an ice bath at -10~-5°C for 100s to obtain an emulsion, and the ultrasonic power is 200W;

[0041] (2) Add 14mL of the second aqueous solution with 10wt% PVA dissolved in the emulsion obtained in step (1), emulsify in an ice bath at -10~-5°C for 100s, and the ultrasonic power is 500W;

[0042] (3) Transfer the emulsion obtained in step (2) to 60 mL of a third aqueous solution in which 1 wt % PVA is dissolved, and magnetically stir for 5 h to remove DCM;

[0043] (4) Centrifuge at 12000r / min for 50min to collect the precipitate, wash with deionized water three times to obtain NaCl+DOX@PLGA nanoparticles.

[0044] The particle size of the prep...

Embodiment 3

[0046] (1) Weigh 200mg mPEG-PLGA and dissolve it in 3mL of dichloromethane (DCM), add it to 0.35mL of the first aqueous solution in which doxorubicin (DOX), NaCl and polyvinyl alcohol (PVA) are dissolved, (PVA The mass concentration is 0.2%, the mass concentration of NaCl is 20mg / mL, and the mass concentration of DOX is 8mg / mL), emulsified in an ice bath at -10~-5°C for 70s to obtain an emulsion, and the ultrasonic power is 170W;

[0047] (2) Add 6mL of the second aqueous solution with 2wt% PVA dissolved in the emulsion obtained in step (1), emulsify in an ice bath at -10~-5°C for 70s, and the ultrasonic power is 300W;

[0048] (3) Transfer the emulsion obtained in step (2) to 20 mL of a third aqueous solution in which 0.05 wt % PVA is dissolved, and magnetically stir for 3 h to remove DCM;

[0049] (4) Centrifuge at 11000r / min for 30min to collect the precipitate, wash with deionized water three times to obtain NaCl+DOX@PLGA nanoparticles.

[0050] The particle size of the p...

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Abstract

The invention discloses microwave sensitization composite nano particles as well as a preparation method and application thereof. The microwave sensitization composite nano particles are hollow PLGA nano particles and wrap NaCL and adriamycin, mPEG-PLGA is dissolved in dichloromethane, a mixed aqueous solution dissolved with polyvinyl alcohol, NaCL and adriamycinis added for emulsification, the dichloromethane is removed in a magnetic stirring manner, and finally the solution is centrifuged, precipitated and cleaned for multiple times, thus obtaining the microwave sensitization composite nanoparticles. The microwave sensitization composite nano particles can be applied to the tumor ablation treatment process so as to improve the sensitivity of tumor cells to a microwave and chemotherapy effect, thereby realizing a synergistic effect of microwave ablation, non-thermal effect and chemotherapy on the tumor.

Description

technical field [0001] The invention relates to a microwave sensitizer, in particular to a microwave sensitization composite nanoparticle, its preparation method and its application in microwave ablation of tumors. Background technique [0002] At present, with the continuous development and improvement of microwave ablation equipment and the breakthrough and innovation of image-guided technology, the curative effect of microwave ablation in the treatment of malignant tumors is becoming more and more sophisticated, especially in the radical treatment of early liver malignant tumors. However, due to the limitation of the heating power of microwave equipment and the influence of carbonization and heat insulation during the ablation process, microwave ablation of large tumors often requires multiple needle insertions, which increases the risk of puncture and costs, and the spatial distribution of the multi-needle thermal field is more complicated. A real-time thermal field with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K31/704A61K9/51A61K47/34A61K47/02A61P35/00
CPCA61K9/5115A61K9/5153A61K31/704A61K41/0038A61K41/0052A61K2300/00
Inventor 梁萍于杰窦健萍孟宪伟周群芳张东云
Owner GENERAL HOSPITAL OF PLA
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