Colorectal cancer early screening primer group based on four genes and kit

Abstract

The invention provides a colorectal cancer early screening primer group based on four genes. The primer group specifically comprises the following primers including 58 pairs of forward and reverse primer groups, 20 pairs of forward and reverse primer groups, 27 pairs of forward and reverse primer groups and 7 pairs of pairs of forward and reverse primer groups, wherein the 58 pairs of forward andreverse primer groups are used for amplifying, covering and detecting whole exon sequence mutation sites of the APC gene, and the nucleotide sequences of the primer groups are as shown in SEQ ID NO:001-116; the 20 pairs of forward and reverse primer groups are used for amplifying, covering and detecting whole exon sequence mutation sites of the CTNNB1 gene, and the nucleotide sequences of the primer groups are as shown in SEQ ID NO:117-156; the 27 pairs of forward and reverse primer groups are used for amplifying, covering and detecting whole exon sequence mutation sites of the B-raf gene, andthe nucleotide sequences of the primer groups are as shown in SEQ ID NO:157-210; the 7 pairs of forward and reverse primer groups are used for amplifying, covering and detecting whole exon sequence mutation sites of the K-ras gene, the nucleotide sequences of the primer groups are as shown in SEQ ID NO:211-224. Exons of the four genes can be completely amplified through the method, all the mutation sites to be detected can be covered, and the accuracy of screening detection is ensured.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a primer set and kit for early screening of colorectal cancer based on four genes. Background technique [0002] Colorectal cancer (CRC) is a malignant tumor originating from the colorectal mucosal epithelium, and is one of the most common malignant tumors in clinic. There are more than 250,000 new cases of colorectal cancer in my country every year, and about 140,000 deaths, accounting for 20% of the world's colorectal cancer cases in the same period. However, at present, the early diagnosis rate of colorectal cancer in my country is very low. Compared with European and American countries, my country still needs to vigorously popularize early screening of colorectal cancer, improve the early diagnosis rate of colorectal cancer in my country, and reduce colorectal cancer-related mortality. [0003] Sporadic colonic adenomas are recognized as precancerous lesions, and the risk of mal...

AI Technical Summary

Problems solved by technology

However, most of these detection kits only detect one or two hotspot mutation gene sites, and the detection sequence is limited to the local sequence of the gene. At present, it has been found that many hotspot mutation genes are closely related to the occurrence and development of colorectal cancer, so these The detection is very easy to cause a high proportion of false negatives, which affects the judgment of the results

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