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Carotenoid-containing composition and preparation method of carotenoid-containing composition

A carotene and composition technology, applied in the field of fermentation engineering, can solve the problems of unstable content and low yield, and achieve the effects of simple preparation method, high nutritional value and improved product quality

Active Publication Date: 2018-05-15
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] ARA usually exists in deep-sea fish oil and some animal tissues. Due to the influence of climate, origin and other conditions, the content is unstable and the output is low, which cannot meet people's needs.

Method used

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  • Carotenoid-containing composition and preparation method of carotenoid-containing composition
  • Carotenoid-containing composition and preparation method of carotenoid-containing composition
  • Carotenoid-containing composition and preparation method of carotenoid-containing composition

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Experimental program
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Effect test

preparation example Construction

[0022] Specifically, the preparation method of the composition may include, for example, the following steps: adding an activator during the fermentation of Mortierella alpina; after the fermentation, collecting the bacteria, separating and extracting oil to obtain a carotenoid-containing composition.

[0023] Alternatively, the Mortierella alpina in the embodiment of the present invention can be activated and cultivated through the following steps, for example: inoculate the Mortierella alpina species in the activation medium, and heat Cultivate under conditions for 46-50 hours to obtain seed activation culture solution. Wherein, the activation medium contains 1-2wt% of yeast powder and 4-8wt% of glucose.

[0024] The seed activation culture solution is inoculated into the expansion medium, and cultured at 26-30°C and 200-240r / min for 46-50h to obtain the seed expansion culture solution. Wherein, the expansion medium contains 1-2wt% of yeast powder and 4-8wt% of glucose.

...

Embodiment 1

[0043] The Mortierella alpina strain was inoculated into the activation medium, and cultured at 26° C. and 200 r / min for 50 h to obtain a seed activation medium. The seed activation culture solution was inoculated into the expansion medium, and cultured at 26° C. and 200 r / min for 50 h to obtain the seed expansion medium. Wherein, both the activation medium and the expansion medium contain 1wt% of yeast powder and 4wt% of glucose, respectively.

[0044] According to the ratio of the inoculum size (volume ratio) of 18:100, the seed expansion culture solution was inoculated in a fermenter equipped with a fermentation medium for fermentation. The fermentation culture process is as follows: the culture temperature is 26°C, the stirring speed is 230r / min, the ventilation volume is 1.0vvm, the tank pressure is normal, and the culture time is 8 days. Wherein, the fermentation medium contains 1.5wt% of yeast powder, 6wt% of glucose, 0.05wt% of potassium dihydrogen phosphate and 0.005...

Embodiment 2

[0048] The Mortierella alpina strain was inoculated into the activation medium, and cultured at 30°C and 240r / min for 46 hours to obtain the seed activation medium. The seed activation culture solution was inoculated into the expansion medium, and cultured at 30° C. and 240 r / min for 46 hours to obtain the seed expansion medium. Wherein, the activation medium and the expansion medium both contain 2wt% of yeast powder and 8wt% of glucose.

[0049] According to the ratio of the inoculum amount (volume ratio) of 22:100, the seed expansion culture solution was inoculated in a fermenter equipped with a fermentation medium for fermentation. The fermentation culture process is as follows: the culture temperature is 30°C, the stirring speed is 270r / min, the ventilation volume is 1.4vvm, the tank pressure is normal, and the culture time is 8 days. Wherein, the fermentation medium contains 2.5wt% of yeast powder, 10wt% of glucose, 0.15wt% of potassium dihydrogen phosphate and 0.015wt% ...

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Abstract

The invention relates to a carotenoid-containing composition and a preparation method of the carotenoid-containing composition, and belongs to the field of fermentation engineering. The preparation method comprises the following steps of adding excitants in the fermentation process of mortierella alpina; after the fermentation is completed, collecting thalli; separating out and extracting oil to obtain the carotenoid-containing composition, wherein the excitants are prepared from ethylene-low polarity oil solution and / or fermentation liquid filter liquid after the self dissolution of blakesleatrispora. The preparation method is simple; the cost is low; the excitants are added into the fermentation process of the mortierella alpina, so that the mortierella alpina which does not originallygenerate carotenoid is stimulated to generate natural carotenoid; ARA and carotenoid can be contained in the fermentation product of the mortierella alpina; the production value of the mortierella alpina is improved; the anti-oxidization capability of the ARA oil is improved. The carotenoid-containing composition provided by the invention simultaneously contains rich ARA and carotenoid; the nutrition value is high; the oxidization stability is higher; the storage and the transportation are convenient.

Description

technical field [0001] The invention relates to the field of fermentation engineering, and in particular to a carotenoid-containing composition and a preparation method of the carotenoid-containing composition, belonging to the field of fermentation engineering. Background technique [0002] Arachidonic acid (ARA) is one of the important fatty acids essential to the human body. It belongs to the ω6 series of long-chain polyunsaturated fatty acids (PUFAs). Various biological activities. [0003] Arachidonic acid is an indispensable substance for the development of the brain and optic nerve during the growth of infants and young children, so it has become an important component in formula milk powder; at the same time, it has also been approved as a nutritional supplement for special diets and prepared milk powder or as a new resource Used in food. In addition, it is the immediate precursor of eicosanoids such as prostaglandins, cycloprostaglandins and leukotrienes. Therefo...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12P7/64C12R1/645
CPCC12P7/6427C12P23/00
Inventor 李翔宇余超陆姝欢潘淼汪志明
Owner CABIO BIOTECH WUHAN CO LTD
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