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Detection kit of A type H7 subtype influenza virus and application of detection kit

A detection kit and influenza virus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that the detection method of A-type H7 subtype influenza virus needs to be improved, and achieve easy operation and promotion, high detection sensitivity and specificity strong effect

Inactive Publication Date: 2018-05-18
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the detection method of A-type H7 subtype influenza virus needs to be improved

Method used

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  • Detection kit of A type H7 subtype influenza virus and application of detection kit
  • Detection kit of A type H7 subtype influenza virus and application of detection kit
  • Detection kit of A type H7 subtype influenza virus and application of detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0077] Using the method of the embodiment of the present invention, the steps of preparing the kit for detecting influenza A and H7 subtype are as follows:

[0078] 1. Buffer preparation

[0079] (1) 0.02M PBS buffer with pH 7.4: Weigh 2.3g Na2HPO 4 , 0.524gNaH 2 PO 4 .H 2 O, 8.77g NaCl was dissolved in pure water, and the volume was adjusted to 1L with pure water, and the pH was adjusted to 7.4 to obtain a 0.02M PBS buffer with a pH of 7.4.

[0080] (2) Membrane treatment buffer: Dissolve Tween-20, BSA, and sucrose in the above 0.02M PBS buffer with a pH of 7.4 to make the mass percentage of Tween-20 0.2% and the mass percentage of BSA 1% , The mass percentage of sucrose is 2%, and the pH is adjusted to 7.4 to obtain a membrane treatment buffer.

[0081] (3) 50mM Borax buffer with pH 8.5: Weigh 1.9g Na 2 B4O 7 .10H 2 O was dissolved in 100ml of pure water, and the pH was adjusted to 8.5 to obtain a 50mM borax buffer with a pH of 8.5.

[0082] (4) Sample treatment solution: Dissolve Tw...

Embodiment 2

[0093] To evaluate the kit for detecting influenza A and H7 subtypes obtained in Example 1, the specific method is as follows:

[0094] 1. Detection sensitivity

[0095] A type H7 subtype influenza virus HI test antigen was used as the test sample to determine the sensitivity of the kit for detecting type A H7 subtype influenza virus of Example 1.

[0096] At the same time, the A type H7 subtype influenza virus HI test antigen was separately and mixed with a pH of 7.4 0.02M PBS buffer to prepare a series of concentrations (64, 32, 16, 8, 4, 2, 1, 0.5, 0.25, 0.125, 0.063, 0HA unit), respectively added to the sample end of the kit for detecting influenza A H7 subtype obtained in Example 1, and detected by a fluorescence detector. Detection steps: Return the sample to be tested to room temperature (25°C) before testing, and use a precise pipette to take 60μl of the sample to be tested and drop it vertically and slowly into the sample end of the kit for detecting influenza A H7 subtype ...

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Abstract

The invention discloses a detection kit of an A type H7 subtype influenza virus and application of the detection kit. The detection kit of the influenza virus comprises: a substrate layer, a first coating film and a second coating film, wherein the first coating film is formed on the substrate layer and is coated with a fluorescence labeled first antibody; the first antibody is in specific bindingwith the A type H7 subtype influenza virus; the second coating film is formed on the substrate layer and is connected with the first coating film; the second coating film is provided with a first region and a second region, which are separated; the first region is located at an end of close to the first coating film; the first region is coated with a second antibody; the second antibody and the first antibody can be in specific binding with the A type H7 subtype influenza virus; the second region is coated with an anti-antibody; the anti-antibody can be in specific binding with the first antibody. The kit disclosed by the invention has the advantages of high detection sensitivity, strong specificity and rapid speed.

Description

Technical field [0001] The present invention relates to the field of virus detection, in particular, to a detection kit for type A H7 subtype influenza virus and its application, more specifically, to a detection kit for type A H7 subtype influenza virus and detection of type A H7 subtype influenza Virus method Background technique [0002] Type A influenza virus, influenza A virus, is a common influenza virus. Influenza A virus is prone to mutation, and its subtypes are often called "bird flu". Avian influenza is a devastating disease that seriously endangers the world's poultry industry. Its pathogen is easily mutated and has multiple serotypes, which makes the prevention and control of the disease difficult. The virus can infect humans after genetic mutation. The symptoms after infection are mainly manifested as follows: High fever, cough, runny nose, myalgia, etc., most of which are accompanied by severe pneumonia. In severe cases, heart, kidney and other organ failures lead...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/569G01N33/577G01N33/533
CPCG01N33/56983G01N33/533G01N33/577
Inventor 马岚吴峰岑瑜
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV