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Cellulose nanocrystal-polyethylenimine nano-compound gene carrier and preparation method thereof

A technology of polyethylenimine and nanocomposites, applied in other methods of inserting foreign genetic materials, recombinant DNA technology, etc., can solve problems such as application constraints and increased cytotoxicity, achieve good biocompatibility, reduce costs, The effect of simple preparation method

Inactive Publication Date: 2018-05-22
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polyethyleneimine (PEI) is used as a cationic gene delivery carrier in biomedical research, but PEI increases the cytotoxicity while improving the gene transfection efficiency, so its application in vivo is restricted

Method used

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  • Cellulose nanocrystal-polyethylenimine nano-compound gene carrier and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Such as figure 1 As shown, a cellulose nanocrystal-polyethyleneimine nanocomposite gene carrier, including a cellulose nanocrystal core and a polyethyleneimine shell coated outside the core, a cellulose nanocrystal core and polyethyleneimine The shell layers are closely matched, and the inner core and the outer shell are made of different materials: the inner core material is cellulose nanocrystal, and the outer shell material is polyethyleneimine.

[0023] Add polyethyleneimine (molecular weight 25kDa) and 1-allyl-3-methylimidazole hydrochloride into the reaction vessel at a mass ratio of 1:20, and mix thoroughly for 1 hour at a temperature of 60°C, and the cellulose nano Crystalline (mass ratio of 0.5:1 to polyethyleneimine) was added into the reaction vessel, stirred and reacted to obtain a cellulose nanocrystal-polyethyleneimine composite. Add epichlorohydrin with a molar ratio of 0.5 times polyacetimide to the complex, and stir and react at a temperature of 95° C....

Embodiment 2

[0025] Add polyethyleneimine (molecular weight 750kDa) and 1-allyl-3-methylimidazole hydrochloride into the reaction vessel at a mass ratio of 1:100, and mix thoroughly for 1 hour at a temperature of 60°C, and the cellulose nano Crystalline (mass ratio of 5:1 to polyethyleneimine) was added into the reaction vessel, stirred and reacted to obtain a cellulose nanocrystal-polyethyleneimine composite. Add epichlorohydrin with a molar ratio of 0.5 times polyacetimide to the composite, and stir and react at a temperature of 95° C. for 240 min to obtain a cellulose nanocrystal-polyethyleneimine nanocomposite gene carrier.

Embodiment 3

[0027] Add polyethyleneimine (molecular weight 100kDa) and 1-allyl-3-methylimidazole hydrochloride into the reaction vessel at a mass ratio of 1:50, and mix thoroughly for 1 hour at a temperature of 60°C, and the cellulose nano Crystalline (mass ratio of 1:1 to polyethyleneimine) was added into the reaction vessel, stirred and reacted to obtain a cellulose nanocrystal-polyethyleneimine composite. Add epichlorohydrin with a molar ratio of 2.5 times of polyacetimide to the composite, and stir and react at 85° C. for 180 min to obtain the cellulose nanocrystal-polyethyleneimine nanocomposite gene carrier.

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Abstract

The invention discloses a cellulose nanocrystal-polyethylenimine nano-compound gene carrier and a preparation method thereof. The cellulose nanocrystal-polyethylenimine nano-compound gene carrier comprises a cellulose nanocrystal inner core and a polyethylenimine shell layer covering the inner core, wherein the cellulose nanocrystal inner core and the polyethylenimine shell layer interacts with each other through electrostatic attraction so as to be tightly combined with each other. The gene carrier disclosed by the invention has the advantages of good biocompatibility and high gene transfection efficiency; moreover, the toxicity of the carrier is reduced, the gene carrier can be recycled, and the gene transfection cost can be reduced. In addition, the preparation method is simple and is low in cost.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a cellulose nanocrystal-polyethyleneimine nanocomposite gene carrier and a preparation method thereof. Background technique [0002] Gene carrier is a tool for transferring genes into cells. Its main function is to carry the target gene into the host cell and enable it to be successfully replicated and expressed. It is widely used in biomedicine, cell engineering, enzyme engineering, molecular biology and other fields . Polyethyleneimine (PEI) is used as a cationic gene transfer carrier in biomedical research, but PEI increases the cytotoxicity while improving the gene transfection efficiency, so its application in vivo is restricted. How to realize the low-toxicity and high-efficiency gene transfection of PEI has become a big problem. [0003] Cellulose nanocrystals are biopolymer materials with simple preparation process, low cost and convenient access. They are environmen...

Claims

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Application Information

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IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 曹诗林古伟明赖林浩王晓君梁世濠杨冰娜
Owner FOSHAN UNIVERSITY
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