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RNA (Ribonucleic Acid) preservation solution and application thereof

A preservation solution and application technology, applied in the field of RNA preservation solution, can solve problems affecting the accuracy and reliability of test results, RNA purification, storage and use difficulties, etc., to prevent RNA degradation, easy storage, and ensure efficiency and accuracy Effect

Active Publication Date: 2018-06-01
GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the quality and integrity of RNA during storage can significantly affect the accuracy and reliability of assay results
RNase, which is widespread and extremely stable in biological samples, has a strong hydrolysis effect on RNA, and many RNases do not require any cofactors to degrade RNA, which brings great difficulties to the purification, storage and use of RNA

Method used

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  • RNA (Ribonucleic Acid) preservation solution and application thereof
  • RNA (Ribonucleic Acid) preservation solution and application thereof
  • RNA (Ribonucleic Acid) preservation solution and application thereof

Examples

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Embodiment 1

[0020] An embodiment of the RNA preservation solution of the present invention comprises ammonium aurintricarboxylate, glycine and bestatin hydrochloride, and the molar amount of the ammonium aurintricarboxylate, glycine and bestatin hydrochloride The ratio is 100:80000:0.02.

Embodiment 2

[0022] An embodiment of the RNA preservation solution of the present invention comprises ammonium aurintricarboxylate, glycine and bestatin hydrochloride, and the molar amount of the ammonium aurintricarboxylate, glycine and bestatin hydrochloride The ratio is 500:200000:0.06.

Embodiment 3

[0024] An embodiment of the RNA preservation solution of the present invention comprises ammonium aurintricarboxylate, glycine and bestatin hydrochloride, and the molar amount of the ammonium aurintricarboxylate, glycine and bestatin hydrochloride The ratio is 300:100000:0.04.

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PUM

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Abstract

The invention discloses an RNA (Ribonucleic Acid) preservation solution. The RNA preservation solution is prepared from aurin ammonium triformate, a preservative and bestatin hydrochloride, wherein the preservative is selected from glycine, epsilon-polylysine and cysteine. The RNA preservation solution provided by the invention can be used for effectively preventing free RNA from being degraded, providing a stable environment for RNA and prolonging the preservation time of the RNA; meanwhile, the subsequent detection efficiency of the RNA and the accuracy of a detection result are not influenced.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an RNA preservation solution and its application. Background technique [0002] Ribonucleic acid (Ribonucleic acid, RNA) is a genetic information carrier present in biological cells and some viruses and viroids. A type of nucleic acid composed of at least dozens of ribonucleotides linked by phosphodiester bonds, named after ribose. Each RNA molecule is made up of long chains of nucleotide units, each containing a nitrogenous base, a ribose sugar, and a phosphate group. Once a biological sample containing RNA is collected and isolated from the body, its RNA will become very unstable and easily degraded. Moreover, RNA is a single-stranded structure, which is easily degraded during the extraction process or storage process. Therefore, the extraction and storage process of RNA needs to be strictly handled to prevent specific or non-specific degradation of RNA. [0003] RN...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/6806
CPCC12N15/10C12Q1/6806C12Q2527/125
Inventor 陈嘉昌陈肖燕柳俊蒋圆玲张瑶
Owner GUANGZHOU JINQIRUI BIOTECHNOLOGY CO LTD
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