Method for determining content of ginsenoside Re in radix ginseng

A technology of ginsenoside and content, applied in the field of chemical detection, can solve problems such as inaccurate measurement results, achieve the effect of improving accuracy, stability and repeatability

Inactive Publication Date: 2018-06-01
WUZHOU INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

[0003] However, during the determination process, due to the excessive pigment impurities in the sample, the determination results are inaccurate. Therefore, it is of great significance to establish an effective removal of impurities and accurate determination of ginsenoside detection methods for the quality control of ginseng and its preparations.

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  • Method for determining content of ginsenoside Re in radix ginseng
  • Method for determining content of ginsenoside Re in radix ginseng

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Embodiment 1

[0024] 1. Instruments and reagents

[0025] 1.1 Instruments:

[0026] Electronic Analytical Balance (XA205DU), ASE350 Fast Solvent Extractor (American DIONEX Company), Agilent1290 High Performance Liquid Chromatography

[0027] 1.2 Reagents (unless otherwise specified, all reagents used in this experiment are of analytical grade):

[0028] Water: Class I water in accordance with GB / T 6682;

[0029] Methanol (CH 3 OH): chromatographically pure (for liquid chromatography).

[0030] 2 methods

[0031] 2.1 Preparation of reference solution:

[0032] Take an appropriate amount of ginsenoside Re reference substance, accurately weigh it, and add methanol to make a solution containing 0.2 mg of ginsenoside Re per 1 ml.

[0033] 2.2 Preparation of the test solution

[0034] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for the test product:

[0035] Take about 0.2g of the powder of this product, weigh it accurately, put it in a Soxhlet extractor, add 30ml...

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Abstract

The invention discloses a method for determining the content of ginsenoside Re in radix ginseng, and belongs to the field of chemical detection. Aiming at the defects, the invention aims to provide the method for determining the content of the ginsenoside in the radix ginseng through an ASE-HPLC method, which effectively combines an ASE (Accelerated Solvent Extraction) method, GCB (Graphitized Carbon Black), and an HPLC (High Performance Liquid Chromatography) method, so that the accuracy and the stability of determination are improved, and the repeatability of the method is improved. The method comprises the steps of adopting the ASE for carrying out methanol extraction on the smashed radix ginseng, mixing 3ml of extract liquor and 8mg of graphitized carbon black, and filtering to obtainradix ginseng extract liquor; adopting the HPLC method for detecting the content of the ginsenoside Re in the radix ginseng extract liquor. The method provided by the invention can be used for controlling the content of the ginsenoside in the radix ginseng and a preparation thereof.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a method for measuring the content of ginsenoside Re in ginseng. Background technique [0002] Ginseng is the most widely used representative of authentic medicinal materials in my country. Ginsenoside is the main medicinal component of ginseng. Ginsenoside has the functions of enhancing memory, improving immunity, improving cardiovascular, regulating endocrine, delaying aging and anti-tumor. Content determination has become a research hotspot at home and abroad. [0003] However, during the determination process, due to the excessive pigment impurities in the sample, the determination results are inaccurate. Therefore, it is of great significance to establish a detection method for effective removal of impurities and accurate determination of ginsenosides for the quality control of ginseng and its preparations. Contents of the invention [0004] For the above-mentioned defici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张云平黄林杰赖秀梅
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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