Culture medium preparation method for straw mushroom strain preservation, culture medium and preservation method thereof

Abstract

The invention relates to a culture medium preparation method for straw mushroom strain preservation, a culture medium prepared by adopting the preparation method and a method for preserving a straw mushroom strain by using the culture medium and belongs to the field of strain preservation. Straws are subjected to specific alkali solution and ultrasonic treatment and then are used for preserving the straw mushroom strain with a common agar culture medium together. Compared with the prior art, the culture medium preparation method provided by the invention has the beneficial effects that the straws are taken as a raw material, raw material cost is low, the culture medium does not need to be preserved at low temperature, cost is further reduced, the culture medium preparation method does notneed instruments such as a refrigerator or high-cost raw materials such as liquid nitrogen and trehalose, and verification shows that preservation time can reach 30 days or more than 30 days and activity is basically not reduced.

Description

technical field [0001] The invention relates to a method for preparing a culture medium for preserving straw mushroom strains, a culture medium prepared by the preparation method and a method for preserving straw mushroom strains using the culture medium, belonging to the field of strain preservation. Background technique [0002] The taste of straw mushroom is delicious, and the meat is crisp and tender. It is a kind of agricultural product loved by the masses. Due to its short production cycle, it only takes about 10 days from sowing to harvesting, and generally 13-20 days to complete a growth cycle. It is one of the edible fungi with the best economic benefits at present. In 2016, the output of straw mushrooms in my country was 213,304 tons, and the output of straw mushrooms in Guangdong Province was 63,000 tons, accounting for about 30% of the national output, ranking first in the country. [0003] Straw mushroom is a high-temperature edible fungus. According to histor...

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Problems solved by technology

[0004] High-quality straw mushroom strains are the basis of straw mushroom production, but the problem of straw mushroom preservation has not been well resolved

However, the mycelium of the vegetative propagule of straw mushroom is prone to variation, aging and degradation due to its rapid growth.

Long-term subculture can also cause germplasm degradation of straw mushrooms, manifested as a decrease in growth rate, a decrease in mycelial density, and a decrease in the production of hydrolytic enzymes.

Moreover, the subculture preservation method needs to be subcultured at intervals, which is cumbersome and has a high pollution rate.

However, the technology of liquid nitrogen preservation method is relatively complicated, and the temperature needs to be lowered programmatically before immersion in liquid nitrogen. The cost of liquid nitrogen container and replenishment of liquid nitrogen is high, which reduces the economic value of straw mushrooms.

Preservatives such as trehalose also have the disadvantages of high price and low economic value.

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