Spiral microchannel, and use method and tandem and parallel mounting method thereof

Abstract

The invention belongs to the field of rare cell and particle enriched screening and relates to a spiral microchannel, and a use method and a tandem and parallel mounting method thereof. For the technical problem of the spiral microchannel in the field of sorting and enrichment of peripheral blood circulating tumor cells of patients suffering from malignant tumor, a tandem and parallel type spiralmicrochannel inertial separation structure capable of performing cascade application of a plurality of spiral microchannels reasonably is cleverly designed; and relative to the existing similar technologies, the tandem and parallel mounting method has the following advantages: high circulating tumor cell capturing efficiency and treatment flux on sorting and enrichment of the peripheral blood circulating tumor cells are achieved, the purity of the circulating tumor cells can be maximized, the loss of the circulating tumor cells can be minimized, the original phenotype of the circulating tumorcells is maintained, the obtained cell suspending liquid containing the high-purity circulating tumor cells is particularly suitable for subsequent biomedicine detection, genetic analysis, cell culture, xenograft tumor preparation and the like, and wide and far-reaching clinical actual application value is achieved.

Description

technical field [0001] The invention belongs to the field of enrichment and screening of rare cells or particles, and relates to a spiral microchannel, its use method and serial and parallel installation methods. Background technique [0002] Invasion and metastasis are the main clinical causes of death in cancer patients, and the death caused by tumor metastases accounts for about 90% of the deaths caused by solid tumors. Therefore, early and accurate detection of tumor metastasis is extremely important. Circulating tumor cells refer to tumor cells that break away from primary and metastatic tumor lesions, invade and enter the circulatory system such as lymphatic vessels and blood. The detection of circulating tumor cells in the circulation system may indicate the existence of tumor micrometastases, and the possibility of further developing into distant metastases. In recent years, many research reports have confirmed the potential value of circulating tumor cells in the e...

AI Technical Summary

Problems solved by technology

but, Existing helical inertial microfluidic chips The main disadvantage of the single-channel helical microchannel inertial microfluidic chip is the low purity of the obtained circulating tumor cells, which leads to great difficulties in the subsequent identification and clinical application of circulating tumor cells. lower value

Although the existing spiral microchannels still have major deficiencies and related technical bottlenecks in sorting and enriching circulating tumor cells, the existing research results suggest that if multiple single The spiral microchannel can greatly improve the purity of circulating tumor cells in the sorted and enriched cell mixture under the premise of ensuring good capture efficiency and processing throughput, so that it can meet the actual clinical needs

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