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Response surface method optimized portulaca oleracea polysaccharide enzyme extraction process

A response surface methodology and extraction process technology, applied in the field of plant polysaccharide extraction process and purslane polysaccharide enzymatic extraction process, to achieve the effect of mild extraction conditions and simple extraction process

Inactive Publication Date: 2018-06-12
陈凌
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There have been some reports on the process of enzymatic extraction of purslane polysaccharides. Enzymatic extraction has many advantages such as rapidity, high efficiency, mild reaction temperature, and easy control. It has been widely used in the extraction of plant polysaccharides, but fresh purslane enzyme There are few reports on the extraction of polysaccharides

Method used

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  • Response surface method optimized portulaca oleracea polysaccharide enzyme extraction process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1, the preparation method of purslane enzymatic hydrolyzate

[0037] Fresh purslane→selection→cleaning→finishing→pressing→enzymolysis→filtering→enzymolysis solution,

[0038] The specific process is:

[0039] (1) Raw material pretreatment: select fresh purslane that is free from rot, pests and damage, and cleaned with tap water, rinsed with pure water, and drained for later use.

[0040] (2) Finishing: Steam finishing for 5 minutes.

[0041] (3) Squeeze: Squeeze with a screw juicer to prevent or reduce air mixing and oxidation of raw materials during the pressing process.

[0042] (4) Enzymolysis: Stir the crushed pulp evenly, carry out enzymolysis extraction with ultrapure water according to a certain slurry ratio at different temperatures, enzymolysis time, and the amount of pectinase added, and pass the single factor test and response surface method Determine the best ratio.

[0043] (5) Filtration: filter the enzymatic solution with four layers of gauze, heat th...

Embodiment 2

[0090] An enzymatic extraction process of purslane polysaccharide, the technological process is fresh purslane→selection→cleaning→finishing→pressing→enzymolysis→filtering→enzymolysis solution, the specific process is as follows:

[0091] (1) Raw material pretreatment: select fresh purslane that is free from rot, pests and damage, and cleaned with tap water, rinsed with pure water, and drained for later use.

[0092] (2) Finishing: Steam finishing for 5 minutes.

[0093] (3) Squeeze: Squeeze with a screw juicer to obtain pulp, and pay attention to preventing or reducing the air in the process of pressing to oxidize the raw material.

[0094] (4) Enzymolysis: Stir the squeezed pulp evenly, add ultrapure water to make the ratio of pulp to water 1:20, add pectinase under the conditions of enzymolysis temperature 44.2°C and pH 5.4 for enzymolysis extraction, enzymolysis The time is 1.5h, and the dosage of pectinase is 0.246g / kg.

[0095] (5) Filtration: filter the enzymolysis sol...

Embodiment 3

[0098] An enzymatic extraction process of purslane polysaccharide, the technological process is fresh purslane→selection→cleaning→finishing→pressing→enzymolysis→filtering→enzymolysis solution, the specific process is as follows:

[0099] (1) Raw material pretreatment: select fresh purslane that is free from rot, pests and damage, and cleaned with tap water, rinsed with pure water, and drained for later use.

[0100] (2) Finishing: Steam finishing for 5 minutes.

[0101] (3) Squeeze: Squeeze with a screw juicer to obtain pulp, and pay attention to preventing or reducing the air in the process of pressing to oxidize the raw material.

[0102] (4) Enzymolysis: Stir the squeezed pulp evenly, add ultrapure water to make the ratio of pulp to water 1:18, add pectinase under the conditions of enzymolysis temperature 45°C and pH 5.0 for enzymolysis extraction, enzymolysis The time is 1.6h, and the dosage of pectinase is 0.25g / kg.

[0103] (5) Filtration: filter the enzymolysis soluti...

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Abstract

The invention relates to a plant polysaccharide extraction process, in particular to a response surface method optimized portulaca oleracea polysaccharide enzyme extraction process, and belongs to thetechnical field of food science and engineering. Based on single factor experiments, analysis is performed according to Design-Expert 8.0.6 software by a Box-Behnken response surface experiment design, a process for extracting polysaccharide from fresh portulaca oleracea juice by pectinase is optimized, an enzymolysis polysaccharide extraction regression model is built, enzyme extracted portulacaoleracea polysaccharide is obtained, the extraction process is simple, extraction conditions are mild, and the process is a proper method for extracting the portulaca oleracea polysaccharide.

Description

technical field [0001] The invention relates to an extraction process of plant polysaccharides, in particular to an enzymatic extraction process of purslane polysaccharides optimized by response surface method, and belongs to the technical field of food science and engineering. Background technique [0002] Purslane (Portulacaoleracea L.) is a 1-year-old succulent herb of the family Portulaca oleracea. It is widely distributed in my country and rich in resources. It is a common Chinese herbal medicine and wild vegetable. It is one of the wild plants with the same origin of medicine and food designated by the Ministry of Health . The biologically active substances in purslane are mainly flavonoids and polysaccharides, among which purslane polysaccharides have the functions of enhancing immunity, anti-tumor, and anti-oxidation. Therefore, the effective extraction of purslane polysaccharides is of great significance to the development and utilization of purslane resources. [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 陈凌
Owner 陈凌
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