Nano composite gene carrier and preparation method thereof

A technology of carboxymethyl cellulose and polyethylene imine, which can be applied to other methods of inserting foreign genetic materials, recombinant DNA technology, etc., can solve problems such as application restrictions and increase cytotoxicity, achieve high biocompatibility, improve Transfection efficiency, cost reduction effect

Inactive Publication Date: 2018-06-12
FOSHAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Polyethyleneimine (PEI) is used as a cationic gene delivery carrier in biomedical research, but PEI increases the cytotoxicity while improving the gene transfection efficiency, so its application in vivo is restricted

Method used

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  • Nano composite gene carrier and preparation method thereof

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Experimental program
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Effect test

Embodiment 1

[0026] Soluble ferrous chloride, ferric chloride and carboxymethylcellulose (calculated as cellobiose residues) are added to the reaction vessel according to 1mol:1.5mol:1mol, and then 100mol1-ethyl-3-methylimidazole is added Glycoacetic acid salt was fully dissolved and dispersed, and ammonia water and epichlorohydrin were added at 60 ° C for 1 hour to obtain magnetic Fe with carboxymethyl cellulose shell. 3 o 4 . 10 g of the obtained magnetic Fe with a carboxymethyl cellulose shell 3 o 4 , 20g of polyethyleneimine (molecular weight: 25kDa) and 40g of epichlorohydrin were added to the reaction vessel, then distilled water was added, and the reaction was stirred at 30°C for 24h to obtain iron ferric oxide-carboxymethyl cellulose with a core-shell structure - Polyethyleneimine nanocomposite gene carrier, such as figure 1 shown.

Embodiment 2

[0028] Soluble ferrous chloride, ferric nitrate and carboxymethyl cellulose (calculated as cellobiose residue) are added to the reaction vessel according to 1mol:2.5mol:1mol, and then 200mol1-ethyl-3-methylimidazolium methyl Acetate is fully dissolved and dispersed, and ammonia water and epichlorohydrin are added to react for 3 hours at 90°C to obtain magnetic Fe with a carboxymethyl cellulose shell. 3 o 4 . 10 g of the obtained magnetic Fe with a carboxymethyl cellulose shell 3 o 4 , 10g polyethyleneimine (molecular weight is 750kDa) and 15g epichlorohydrin are added to the reaction vessel, then distilled water is added, stirred and reacted at 30°C for 4h, and ferric oxide-carboxymethyl cellulose with core-shell structure is obtained - Polyethyleneimine nanocomplex gene carrier.

Embodiment 3

[0030] Soluble ferrous nitrate, ferric sulfate and carboxymethyl cellulose (calculated as cellobiose residues) are added to the reaction vessel according to 1mol:2.5mol:2mol, and then 55mol1-ethyl-3-methylimidazoliummethyl ethyl The acid salt is fully dissolved and dispersed, and ammonia water and epichlorohydrin are added to react for 1.5 hours at 75°C to obtain magnetic Fe with a carboxymethyl cellulose shell. 3 o 4 . 10 g of the obtained magnetic Fe containing carboxymethyl cellulose shell 3 o 4 , 20g polyethyleneimine (molecular weight is 100kDa) and 20g epichlorohydrin are added in the reaction container, then add distilled water, stir and react at 30 ℃ for 0.5h, obtain ferric oxide-carboxymethyl fiber with core-shell structure DNA-polyethyleneimine nanocomposite gene carrier.

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Abstract

The invention discloses a nano composite gene carrier and a preparation method thereof. The gene carrier comprises a magnetic nano core, a shell 1 and a shell 2, the shell 1 and the shell 2 coat the magnetic nano core which is closely matched with the shell 1, the shell 1 and the shell 2 are closely matched, the magnetic nano core is made of Fe3O4, the shell 1 is made of, and the shell 2 is made of. The gene carrier has magnetic response characteristics, guidability of gene transfection is enhanced, gene transfection efficiency is improved, and the gene carrier has high biocompatibility and high gene transfection efficiency, is recyclable and can lower cost of gene transfection.

Description

technical field [0001] The invention relates to the field of biological materials, in particular to a ferric oxide-carboxymethyl cellulose-polyethyleneimine nanocomposite gene carrier and a preparation method thereof. Background technique [0002] Gene carrier is a tool for transferring genes into cells. Its main function is to carry the target gene into the host cell and enable it to be successfully replicated and expressed. It is widely used in biomedicine, cell engineering, enzyme engineering, molecular biology and other fields . However, common gene carriers are not recyclable, which increases the application cost of genetic engineering. The main reason why the gene carrier is difficult to recover is that the gene carrier is not magnetically responsive, and after gene transfection, it cannot be enriched and recovered in the presence of a magnetic field. Therefore, there is an urgent need to develop a magnetically responsive gene carrier to improve the recyclability of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/87
CPCC12N15/87
Inventor 曹诗林古伟明赖林浩王晓君梁世濠杨冰娜
Owner FOSHAN UNIVERSITY
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