Application of male sea horse to preparation of medicine or preparation for resisting depressive behavior
An anti-depression and hippocampus technology, applied in the field of medicine, can solve problems such as unclear molecular mechanisms, and achieve the effect of improving depression-like behaviors
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Embodiment 1
[0061] Example 1 Establishment of mouse depression model
[0062] Refer to Papp and Willner (Papp et al. , 1992) and modified for applicability, the animals were repurchased and adapted to the laboratory for one week, and randomly numbered and divided into groups, a total of 4 groups were set up, as shown in Table 1:
[0063] Table 1 Grouping of experimental animals
[0064]
[0065] The CUMS group and the CUMS+Seahorses group were exposed to the following stressors as model treatments; the Control group and Seahorses groups were reared under standard conditions without stress treatment.
[0066] All the stressors were randomly operated independently, and it was guaranteed not to be repeated within a week to prevent the animals from adapting. Two stressors were operated independently every day, and after 8 weeks of continuous stress, the behavior was measured. Stress sources include: water deprivation for 12 hours followed by stimulation with an empty water bottle for 6 ...
Embodiment 2
[0067] Embodiment 2 Behavioral experiments
[0068] The mice under four kinds of treatments in embodiment 1 are carried out following behavioral experiment:
[0069] 1. Social experiment
[0070] 1. Steps
[0071] The three-box social test box device is composed of three rectangular boxes, each box is 19×45cm in size, the partition between each box is transparent resin glass, and there is a channel in the middle to connect the three boxes, on the left and right A metal cage of the same specification is placed in the center of the side boxes, and the size should be large enough to accommodate a mouse. By measuring the number of times the mouse enters each box and the duration within 10 minutes (when the mouse's head and four paws enter a box, it is considered to be in that box), and the social mouse metal cage / empty cage within 10 minutes The number and duration of direct contact are used to analyze the social situation.
[0072] 2. Results
[0073] Such as figure 2 As s...
Embodiment 3
[0090] Example 3 Effect of male hippocampus on the expression of related proteins in mouse hippocampus
[0091] The expression of CD11b, a marker of M1 phenotype of microglial cells, and BDNF protein, a neurotrophic factor, were detected by Western Blot technology. The specific experimental steps are as follows:
[0092] 1. Isolation of mouse hippocampal brain tissue
[0093] After the mice were decapitated and sacrificed, the hippocampal tissues were quickly isolated on ice, frozen in liquid nitrogen, and then transferred to -80°C for storage.
[0094] 2. Preparation of protein samples
[0095] 1. Wash the hippocampal tissue with PBS solution at 4°C, then place it in a 1.5mL centrifuge tube, add protein lysate to the tissue, and use a hand-held homogenizer to homogenize. The amount added is related to the weight of the brain tissue. The volume mass ratio of lysate to tissue weight is 1mL:5g, and the whole process of homogenization is carried out on ice.
[0096] 2. Place ...
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