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A kind of biological monitoring method of water pollution

A biological monitoring and water pollution technology, applied in the biological field, can solve the problems of affecting the reliability of the judgment results, increasing the difficulty of monitoring, and affecting the difference, achieving good social benefits, eliminating background differences, and easy operation

Active Publication Date: 2020-11-10
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the evaluation criteria involve the comparison of indicator biological traits between the pollution group and the control group, this aggravates the difficulty of monitoring
On the other hand, the indicator organisms of the pollution group and the control group come from different individuals, and individual differences will inevitably affect the differences between the groups, affecting the reliability of the evaluation results

Method used

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  • A kind of biological monitoring method of water pollution
  • A kind of biological monitoring method of water pollution
  • A kind of biological monitoring method of water pollution

Examples

Experimental program
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Effect test

Embodiment 1

[0009] Embodiment 1: a kind of biological monitoring method of water pollution, comprises the following steps: large yellow croaker is taken from local fishing ground, and indoor maintains in the plastic tank of 500L, adapts to 2 weeks, throws something and feeds commercial feed (48% of crude protein, 11% of crude fat) every day. %), fed twice a day (at 9 o'clock in the morning and at 4 o'clock in the afternoon). When the experiment started, large yellow croakers with a body weight of 110-130 g were selected and exposed to zinc conditions of 0 and 5 mg / L for 12 hours, wherein the water temperature was 25.9 ± 2.7℃, photoperiod 14L:10D, dissolved oxygen 6.43±0.56mg / L, pH value 7.49±0.52, salinity 32.6±1.5, hardness 125mg / L CaCO3; The crude fat content of the liver lobe was measured, and the results are shown in Table 1. It can be seen from the table that the crude fat content of the right liver lobe of large yellow croaker was significantly lower than that of the left liver lobe ...

Embodiment 2

[0012] Embodiment 2: a kind of biological monitoring method of water pollution, comprises the following steps: large yellow croaker is taken from local fishing ground, and indoor maintains in the plastic barrel of 500L, adapts to 2 weeks, throws something and feeds commercial feed (crude protein 48%, thick crude fat 11%), fed twice a day (at 9 o'clock in the morning and at 4 o'clock in the afternoon). When the experiment started, large yellow croakers with a body weight of 110-130 g were selected and exposed to zinc conditions of 0 and 5 mg / L for 20 hours, wherein the water temperature was 25.9 ± 2.7℃, photoperiod 14L:10D, dissolved oxygen 6.43±0.56mg / L, pH value 7.49±0.52, salinity 32.6±1.5, hardness 125mg / L CaCO3; The left and right liver lobes of yellow croaker were measured for crude fat content. The results are shown in Table 2. It can be seen from the table that the crude fat content of the right liver lobe of large yellow croaker was significantly lower than that of the ...

Embodiment 3

[0015] Embodiment 3: a kind of biological monitoring method of water pollution, comprises the following steps: large yellow croaker is taken from local fishing ground, and indoor maintains in the plastic tank of 500L, adapts to 2 weeks, throws something and feeds commercial feed (crude protein 48%, thick crude fat 11%), fed twice a day (9 o'clock in the morning and 4 o'clock in the afternoon). When the experiment started, large yellow croakers with a body weight of 110-130 g were selected and exposed to 0 and 5 mg / L zinc conditions for 4 days, and the water temperature was 25.9 ± 2.7℃, photoperiod 14L:10D, dissolved oxygen 6.43±0.56mg / L, pH value 7.49±0.52, salinity 32.6±1.5, hardness 125mg / L CaCO3; The left and right liver lobes of yellow croaker were observed, and the results are shown in Table 2. It can be seen from the table that after exposure to zinc-polluted water for 4 days, there were significant differences between the left and right liver lobes of large yellow croake...

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Abstract

The present invention discloses a new water pollution biological monitoring method, which is based on different responses of left and right liver lobes of a large yellow croaker individual to water pollution to determine whether water is contaminated with zinc. The beneficial effects are that: the method eliminates background difference of a control group, is accurate, reliable, simple and quick,and is suitable for application. By comparing differences of the crude fat content of the left and right liver lobes, the water is monitored. The method can not only judge whether the water is polluted, but also can reflect the harmfulness of the polluted water to organisms. Through the color of the left and right liver lobes, whether the water is contaminated with the zinc can be judged. The operation is simple, the time is shortened, the labor efficiency is improved, and the cost is greatly reduced.

Description

technical field [0001] The invention relates to the field of biological technology, in particular to a water pollution biological monitoring method. Background technique [0002] Water body biological monitoring is to use the response of biological components, individuals, populations or communities to water environmental pollution or environmental changes, and provide a basis for the monitoring and evaluation of water environmental quality from a biological point of view. The method of previous research is to evaluate the environmental pollution by comparing the traits of indicator organisms in normal water bodies and polluted water bodies. The biological indicator method of this kind of water environment must consider the influence of the control group. In the polluted water body, the indicator organisms will show certain abnormal traits, but the basis for judging the abnormality must have the traits of the indicator organisms in the normal water body as a reference. Sin...

Claims

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Application Information

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IPC IPC(8): G01N33/18
CPCG01N33/186Y02A20/20
Inventor 郑家浪
Owner ZHEJIANG OCEAN UNIV
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