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Preparation method and application of graphene oxide anti-tumor drug targeting vector

An anti-tumor drug and graphene technology, applied in anti-tumor drugs, drug delivery, drug combination, etc., can solve the problems of inability to reach the dose of lethal cancer cells, limited drug loading, and no clinical application value, etc., and achieve easy modification , large surface area and high stability

Inactive Publication Date: 2018-06-22
GUANGXI UNIV OF CHINESE MEDICINE
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Problems solved by technology

[0004] However, the traditional application of monoclonal antibodies, hormones, and liposomes as anticancer drug carriers has not achieved obvious clinical application value
Moreover, the drug load is limited, and the dose that can kill cancer cells cannot be reached

Method used

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  • Preparation method and application of graphene oxide anti-tumor drug targeting vector
  • Preparation method and application of graphene oxide anti-tumor drug targeting vector
  • Preparation method and application of graphene oxide anti-tumor drug targeting vector

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preparation example Construction

[0029] A preparation method for a graphene oxide antitumor drug targeting carrier, comprising the steps of:

[0030] (1) Dissolving the DNA single strand in PBS buffer solution, ultrasonically dissolving, and then adding the complementary strand of the upper DNA single strand to the above-mentioned cooled solution to perform base complementary pairing of double-stranded DNA molecules;

[0031] (2) Graphene oxide is added in the PBS buffer solution, ultrasonically dissolved, then the DNA solution of complementary base pairing in step (1) is added in the solution graphene oxide solution, stirring and mixing;

[0032] (3) Add the antitumor drug to the mixed solution in step (2), stir and mix evenly, and centrifuge to obtain a precipitate that is the graphene oxide antitumor drug targeting carrier.

[0033] Preferably, in the steps (1) and (2), the concentration of the PBS buffer solution is 0.01 mol / L, and the pH is 7.0-8.0.

[0034] Preferably, in the step (1), in a thermostat,...

Embodiment 1

[0041] 0.01M phosphate buffer: weigh 8g NaCl, 0.2g KCl, 1.44g Na 2 HPO 4 and 0.24g KH 2 PO 4 , dissolved in 800ml of distilled water, adjusted the pH value of the solution to 7.4 with HCl, and finally added distilled water to make the volume to 1L. Steam sterilize at 15lbf / in2 (1034×105Pa) under high pressure (at least 20 minutes), and store at room temperature or in a refrigerator at 4°C.

[0042] (1) Dissolve 10 μL ssDNA (10 μM) in 0.01M PBS buffer solution, ultrasonically dissolve, heat to 95°C in a thermostat and keep it for 10 minutes, then naturally cool to 25°C;

[0043] Then add 10 μL, 10 μM) complementary strands of ssDNA into the above-mentioned cooled solution for base complementary pairing, the temperature of base complementary pairing is 25°C, and the reaction time is 24 hours, to obtain double-stranded DNA molecules;

[0044] (2) 0.2g graphene oxide is added in 0.01M PBS buffer solution, sonication is dissolved, and then the DNA solution of complementary base...

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Abstract

The invention relates to a preparation method of a graphene oxide anti-tumor drug targeting vector. The preparation method comprises the following steps: (1) dissolving a deoxyribonucleic acid (DNA) single chain into a phosphate buffer solution (PBS), and carrying out ultrasonic dissolution; then, adding a complementary chain of the DNA single chain into the cooled solution for carrying out complementary base pairing so as to obtain double-chain DNA molecules; (2) adding graphene oxide into the PBS, and carrying out ultrasonic dissolution; then, adding the DNA solution, subjected to the complementary base pairing in the step (1), into a solution in which the graphene oxide is dissolved, and evenly stirring and mixing; (3) adding an anti-tumor drug into the mixed solution obtained in the step (2), evenly mixing and stirring, and then centrifuging to obtain precipitate, i.e., the graphene oxide anti-tumor drug targeting vector. The anti-tumor drug targeting vector has a targeting effecton tumor cells and can accurately convey a drug to a specific location and kill the tumor cells.

Description

technical field [0001] The invention relates to the technical field of nanomedicine, in particular to a preparation method and application of a graphene oxide anti-tumor drug targeting carrier. Background technique [0002] Cancer is considered by the World Health Organization to be the "first killer" of human beings in the 21st century. Therefore, cancer control has become the focus of global health strategies. At present, China has become the world's largest cancer country. Cancer not only seriously threatens people's life and health, but also causes a heavy burden to families, society and the country. According to the statistical data in 1994, cancer accounted for 21.82% and 16.50% of the total death causes in urban and rural populations in my country, rising from the third and fourth places in the 1970s to the second place, and the most common cancer among people aged from 54 to 54. The best working age, cancer has long been the first cause of death among all kinds. In ...

Claims

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Application Information

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IPC IPC(8): A61K9/00A61K47/04A61K47/26A61K31/704A61P35/00
CPCA61K9/00A61K31/704A61K47/02A61K47/26
Inventor 赵立春
Owner GUANGXI UNIV OF CHINESE MEDICINE
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