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Use of preparations which takes cress total phenolic acid as raw material medicine for preparing anti-human immunodeficiency virus drugs

A technology for pharmaceutical preparations and HIV, applied in the field of separation and purification of effective parts of plants, can solve the problems of low yield of total phenolic acid, large loss of components, destruction of components, etc., and achieve clear effect, increase percentage, and increase CD4+/CD8+ effect of value

Inactive Publication Date: 2018-06-22
黄正明 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used extraction and separation processes are: alcohol extraction-water precipitation-ethyl acetate extraction method or acid-base method to obtain total phenolic acids. These methods lose a lot of active ingredients or components are destroyed, resulting in low yield and high cost of total phenolic acids

Method used

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  • Use of preparations which takes cress total phenolic acid as raw material medicine for preparing anti-human immunodeficiency virus drugs
  • Use of preparations which takes cress total phenolic acid as raw material medicine for preparing anti-human immunodeficiency virus drugs
  • Use of preparations which takes cress total phenolic acid as raw material medicine for preparing anti-human immunodeficiency virus drugs

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0018] Experimental example 1: Inhibitory effect of cress total phenolic acid on HIV in vitro

[0019] 1. Experimental materials

[0020] 1.1 Experimental cells TZM-B1 cells, derived from HeLa cell line, can infect various tropisms of HIV, simian immunodeficiency virus (SIV) and simian-human immunodeficiency virus (sHIV), etc. Viruses can infect.

[0021] 1.2 Virus The experimentally adapted strain SF33 virus was provided by the Chinese Center for Disease Control and Prevention.

[0022] 1.3 Reagents DMEM medium, CORNING; FBS fetal bovine serum, Gibco; glutamine (G)L-Glutamine, Gibco; penicillin, streptomycin, Gibco; Britelite TM plus Reporter Gene Assay System PerkinElmer6066769.

[0023] 1.4 Experimental instruments Carbon dioxide incubator, Thermo scientific HERAcell 150i; optical microscope, OLYMPUS CKX41; blood cell counter, NIHON KOHDEN MEK-6318K; cell luminescence detector, PerkinElmer 1420 Multilabel Counter.

[0024] 2 Test method

[0025] 2.1 Inoculate TZM-bl ce...

experiment example 2

[0038] Experimental example 2: Therapeutic effect of cress total phenolic acid on mouse retrovirus (Fr.MuLV) infection model

[0039] 1 Materials and methods

[0040] 1.1 Animals 48 BALB / c mice, SPF grade, body weight 17-19g, half male and half male (purchased from Beijing Weitong Lihua Experimental Animal Technology Co., Ltd., animal certificate number: SCXK (Beijing) 2012-0001.)

[0041] 1.2 Reagents Total phenolic acid of cress, extracted by this research institute (batch number: 090303); Zidovudine tablets, product of Northeast Pharmaceutical General Factory (batch number: 5151102); Mouse CD 4 ELISA Kit (batch number: 201509), Mouse CD 8 ELISA Kit (both provided by Shanghai Enzyme Biotechnology Co., Ltd.); Trizol (Invitrogen Company, batch number: 90803); chloroform (Tianjin Fengchuan Chemical Reagent Technology Co., Ltd., batch number: 20150226); isopropanol (Tianjin East China Reagent Factory, batch number: 20140915); 75% ethanol (produced by Wuhan Xingheda Trading Co....

experiment example 3

[0072] Experimental example 3: Inhibitory effect of cress total phenolic acid on HIV reverse transcriptase activity in vitro

[0073] 1 Experimental materials

[0074] 1.1 Drugs

[0075]Total phenolic acids of cress, self-extracted by Huang Zhengming's research group; nevirapine (NVP), Haidi Sanuo pharmaceutical.

[0076] 1.2 HIV reverse transcriptase

[0077] HIV reverse transcriptase (purchased from Ambion).

[0078] 1.3 Reagents

[0079] Dimethyl sulfoxide (DMSO), BIOMOL; bovine serum albumin (BSA), Beijing Zhongmei Transduction Technology; DTT, PolyA, PNPP, SIGMA; dUTP, Shanghai Sangong; dTTP, oligo(dT) 15 , Dalian Biodee; Tris, Beijing Biodee; alkaline phosphatase-labeled streptavidin (APS), Zhongshan Jinqiao; MgCl 2 ·6H 2 O, KCl, NaOH, Beijing Chemical Plant.

[0080] 1.4 Main Instruments

[0081] 96-well cell culture plate, NUNC Company, Denmark; low-temperature high-speed centrifuge, SIGMA 3-18K; electronic analytical balance, Shanghai Yuhengping Scientific Ins...

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Abstract

The invention discloses use of preparations which takes cress total phenolic acid as a raw material medicine for preparing anti-human immunodeficiency virus drugs. The raw material medicine is extracted from the cress total phenolic acid, and the raw material medicine contains 55-80% of total phenolic acid. Through vitro experiments, the cress phenolic acid has strong anti-HIV activity against HIV; in vivo experiments, the cress total phenolic acid increases percentage of CD4+ T cells, increases the CD4+ / CD8+ value, and improves the immunosuppressive effects of the body to play an anti-virus role; the cress total phenolic acid has a good inhibitory effect on the activity of HIV reverse transcriptase. The cress total phenolic acid has the anti-HIV effect in vivo and in vitro, the effect isclear, the anti-HIV effect is achieved by inhibiting the activity of HIV reverse transcriptase, the mechanism of action is clear, and the potential for clinical use development is achieved.

Description

technical field [0001] The invention belongs to the technical field of separating and purifying the effective parts of plants, and can make the total phenolic acid content of the effective parts of cress greater than 50%, reaching the national standard of 5 kinds of raw materials of traditional Chinese medicine; use. Background technique [0002] The team of the present inventors has studied a preparation process of total phenolic acids of cress and a pharmaceutical preparation and application using the total phenolic acids as an active ingredient (patent number 2006100838103) and a preparation process of total phenolic acids of cress and the composition It is used as the pharmaceutical preparation and liver disease application of raw materials (Patent No. 2006101607233), and the extraction process and application of total phenolic acids of cress are initially explored. Commonly used extraction and separation processes are: alcohol extraction-water precipitation-ethyl aceta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/23A61P31/18
CPCA61K36/23A61K2236/15A61K2236/333A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 黄正明刘青川曹文斌
Owner 黄正明
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