33results about How to "Play an antiviral role" patented technology

The invention discloses a shuttling intracellular antibody TAT-4F for H3N2-type canine influenza virus. The H3N2 virus is A-type influenza virus, the main neutralizing antibody is from hemagglutinin (HA), therefore, the HA becomes an original main research target; then the influenza virus has high variability, the immune cross reaction among different variable branches is weaker, M1 antibody can be combined with M1 protein to inhibit the activity and disturb multiplication, transcription and release of the influenza virus so as to play a role in resisting the virus; therefore, the M1 protein is selected for preparing the corresponding antibody to obtain stable titer, coupling the antibody with TAT protein PTD (Protein Transduction Domain) capable of transducing biomacromolecules into cellsand expressing fusion protein TAT PTD-M1 ScFv, and then a shuttling antibody for resisting the canine influenza virus is prepared to provide a new path for treating the canine influenza virus.

The present invention relates to an andrographolide sodium sulfonate freeze-dried powder injection preparation. Said freeze-dried powder injection preparation contains unit dose 10-1500 mg of andrographolide sodium sulfonate, and at least contains one pharmaceutically-acceptable carrier, said carrier includes excipient, pH regulating agent, anti-oxidant and stabilizing agent. Said invented powder injection preparation can be mainly used for curing the diseases of bronchitis, cystauchenitis, urethritis, pelvic inflammation, acute icterohepatitis anjd prostatitis, etc. Besides, said invention also provides its preparation process.

The invention discloses a shuttling intracellular antibody for H3N2-type canine influenza virus. The H3N2 virus is A-type influenza virus, the main neutralizing antibody is from hemagglutinin (HA), therefore, the HA becomes an original main research target; however the influenza virus has high variability, the immune cross reaction among different variable branches is weaker, M1 antibody can be combined with M1 protein to inhibit the activity and disturb multiplication, transcription and release of the influenza virus so as to play a role in resisting the virus; therefore, the M1 protein is selected for preparing the corresponding antibody to obtain stable titer, coupling the antibody with TAT protein PTD (Protein Transduction Domain) capable of transducing biomacromolecules into cells andexpressing fusion protein TAT PTD-M1 ScFv, and then a shuttling antibody for resisting the canine influenza virus is prepared to provide a new path for treating the canine influenza virus.

The invention belongs to the pesticide filed and in particular relates to a plant virus preventing agent which is not only capable of preventing the plant viruses, but also capable of promoting the yield increase. The specific drug consists of moroxydine hydrochloride, cantharidin, ammonium sulfate and DA (Dopi Amine)-6.

The invention relates to the technical field of medical materials, and provides a preparation method for an antibacterial and antiviral protective material. The preparation method for the antibacterial and antiviral protective material comprises the steps of (1) adding polyvinyl alcohol and polylysine into de-ionized water of 90 to 100 DEG C, stirring and dissolving to prepare a solution A with the mass fraction of 8 to 12 percent; (2) dissolving a natural plant extracting liquid, a softening agent, and an emulsifying agent into an organic solvent to prepare a solution B; (3) slowly adding thesolution B to the solution A, and after the solution B is added completely, continuing stirring for 20 to 40 minutes to obtain a stable emulsion; and (4) performing electronic spinning on the emulsion obtained in the step (3) to prepare a protective material for isolating viruses or bacteria. The preparation method for the antibacterial and antiviral protective material solves the problems that the medical protective material in the prior art can only isolate the bacteria and the viruses, and does not have the functions of resisting the bacteria and germs.

The invention discloses an environment-friendly antibacterial antiviral mildew-proof integrated interior wall coating which is prepared from the following raw materials in parts by mass: 200-300 parts of shell powder; the invention also provides a preparation method of the environment-friendly antibacterial antiviral mildew-proof integrated interior wall coating, and the preparation method comprises the following steps: S1, material preparation: preparing raw materials by a worker, and preparing the raw materials into required materials; the environment-friendly, antibacterial, antiviral and mildew-proof performance of the coating is improved by replacing materials for traditional coating production and adding new materials, chemical raw materials used in the past are reduced by adding natural materials, meanwhile, formaldehyde can be effectively adsorbed and converted into harmless substances, and the environment-friendly, antibacterial, antiviral and mildew-proof performance of the coating is improved. Meanwhile, the antibacterial and antiviral effects can be well achieved under visible light and ultraviolet light, strong antibacterial and antiviral effects can be achieved through nano-silver under the condition that no light source exists, mildew breeding can be avoided through natural materials, mildew can be prevented, it is guaranteed that the antibacterial and antiviral effects are achieved all day long, and the indoor environment quality is improved.

The invention relates to a cinnamyl aldehyde derivative with good stability and prominent performances and application thereof. The structural formula of the compound is shown as the description. The derivative can be obtained by a heterocyclic compound containing an amino and cinnamyl aldehyde through a condensation reaction. The cinnamyl aldehyde derivative provided by the invention has antibacterial, corrosion-resisting, anti-virus functions and the like, and also has the characteristics of high stability, mildness and the like, so that the cinnamyl aldehyde derivative can replace the cinnamyl aldehyde to be wildly applied to fields including daily chemical products, foods, medicine, feed and the like.

The invention provides an infectious bovine rhinotracheitis virus (IBRV) aptamer. The aptamer has the maximum binding force and favorable specificity, can inhibit the virus infection on cells to some extent, and can be used for indirect immunofluorescent assay (IFA) after being marked by FAM. The aptamer can be used for inhibiting the virus infection on cells to perform the antivirus action, and can also be used as an anti-IBRV probe or target spot for preparing anti-IBRV drugs or reagents.

The invention relates to an application of lariciresinol-4-O-beta-D-glucoside in the preparation of drugs for treating or preventing flu and related inflammatory diseases caused by influenza virus. The application of lariciresinol-4-O-beta-D-glucoside in clinical treatment of influenza virus diseases has characteristics of safety, effectivity and little toxic or side effect. The invention also provides a pharmaceutical composition, an active ingredient of which comprises lariciresinol-4-O-beta-D-glucoside. By cytopathic-effect inhibition assay, it is verified that lariciresinol-4-O-beta-D-glucoside has an effect of inhibiting replication of influenza virus in the treatment mode. The result proves that lariciresinol-4-O-beta-D-glucoside has obvious effect of inhibiting influenza virus in the treatment mode. Selection index SI is greater than 4. The lariciresinol-4-O-beta-D-glucoside has advantages of low toxicity and high effectiveness.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a natural plant active factor antibacterial atomizing agent and a preparation method of the antibacterial atomizing agent. The natural plant active factor antibacterial atomizing agent comprises the following components: white mulberry root-bark, honeysuckle, cordyceps sinensis, saussurea involucrata,platycodon grandiflorum, radix ophiopogonis, radix pseudostellariae, green tangerine peel, micromolecular plant acid extract and deionized water. The preparation method comprises the following steps:mixing the cortex mori radicis, the honeysuckle, the cordyceps sinensis, the saussurea involucrata, the platycodon grandiflorum, the radix ophiopogonis, the green tangerine peel and the radix pseudostellariae in parts by weight, soaking and extracting the mixture with ethanol, and filtering and concentrating the filtrate for later use. The traditional Chinese medicine preparation for plant extraction of small molecules provided by the invention adopts atomization of particles smaller than 5 microns, and the particles are permeated and absorbed by respiratory mucosa of a human body to enhance cellular immune function and play an antiviral role.

The invention discloses antiviral flame-retardant weather-resistant coating. The coating is characterized in that the coating comprises, by weight, 2 to 5 parts of epoxy hexamethylene tetramine salt,30-40 parts of a 4-propenylthiosemicarbazide / 1-allyl-3-methylimidazolium chloride / zeatin nucleoside / isobornyl acrylate copolymer, 5-8 parts of modified Ti-Cu-Hf-O, 1-3 parts of antiviral Chinese herbal medicine powder, 10-15 parts of an inorganic filler, 10-15 parts of a solvent, 1-3 parts of a dispersing agent and 0.5-1.5 parts of a defoaming agent. The invention also discloses a preparation method of the antiviral flame-retardant weather-resistant coating. The antiviral flame-retardant weather-resistant coating disclosed by the invention is excellent in comprehensive performance, remarkablein antiviral effect, good in weather resistance and flame resistance, high in bonding strength with a base material and long in service lifetime.

The invention provides an antiviral traditional Chinese medicinal slow-release granule for aquaculture and a preparation method of the traditional Chinese medicinal slow-release granule. The antiviraltraditional Chinese medicinal slow-release granule is formed by reasonable matching and has the efficacy of clearing heat and detoxicating, resisting bacteria and eliminating inflammation and the like. The test proves that the traditional Chinese medicinal slow-release granule disclosed by the invention has the functions of resisting viruses, inhibiting germs and the like, and has the advantagesof high safety, no residues in aquatic animal bodies and no drug resistance; in addition, the formula is reasonable and simple and the cost is low; the antiviral traditional Chinese medicinal slow-release granule is especially suitable for culturing grass carp, crucian carp and carp; by coating the surface of a core material with a wall material, the drug properties are slowly released, and the dosage is reduced, so that lower economic cost and smaller influence on a water body are realized. By carrying different traditional Chinese medicinal materials of the core material, different slow-release granules for different fishes can be developed in a targeted manner, and have high pertinence and small influence on other fishes. By arranging gypsum and lard oil, the slow-release granule can bekept in agglomeration in water and suspend in the water body; the drug is slowly released and further favorably attracts the fishes to eat. The folium isatidis and herba houttuyniae can resist viruson the one hand and can achieve the effect of feeding attraction on the other hand.

The invention relates to a nutritional agent for promoting the growth of Pinus thunbergii seedlings. The nutritional agent is prepared from urea, potassium chloride, compound sodium nitrophenolate, calcium superphosphate, potassium dihydrogen phosphate, borax, dried scallop, prsimmon calyx, broadleaf holly leaf, Caulis Sargentodoxae, Euonymus alatus, phoenix tree flower, lotus leaves, clay rocks, medical stones, muscovite, sodium selenite, auximone and polyaspartic acid. The nutritional agent for promoting the growth of Pinus thunbergii seedlings has the advantages of high utilization rate, increase of the production speed of the seedlings, economic benefit increase, enhancement of the disease and drought resistance of the seedlings, simple preparation method, easiness in operation, low device requirements, less investment, and realization of industrial production.

The invention discloses enteric-coated capsules comprising a blue turmeric rhizome extract. The capsules comprise microencapsulated liposome and ultrafine blue turmeric rhizome oil. Compared with prior arts, the capsules provided by the invention have the advantages that: with an ultrafine crushing process and a high-speed centrifugal separation method, blue turmeric rhizome raw material original physiochemical properties are maintained, bioavailability is high, and blue turmeric herb active components are preserved; ultrafine blue turmeric rhizome oil is subjected to microencapsulated liposome coating, such that blue turmeric rhizome oil volatility is controlled, blue turmeric rhizome oil content stability is maintained, and blue turmeric rhizome oil oxidation and deterioration are prevented; the liposome has high targeting property, such that blue turmeric rhizome oil anti-inflammatory and antibacterial functions are utilized and developed; microencapsulated catechin-tea flavonoid crystals are added, such that anti-inflammatory and antibacterial capacities of the product provided by the invention can be developed, and an antiviral effect can be provided; silver ions are added as an absorbent and disinfectant, such that catechin is prevented from being corroded, and catechin antibacterial and antiviral functions can be developed.

The invention discloses a bacteriostatic and moisturizing camellia congealed fat cold-processed essential oil soap, and the oil soap is prepared from the following raw material components: 3 to 6 parts of camellia essential oil, 1 to 3 parts of rosemary essential oil, 1 to 3 parts of aromatic essential oil, 2 to 4 parts of marigold essential oil, 2 to 4 parts of lavender essential oil, 1 to 3 parts of palmarosa essential oil, 1 to 3 parts of vetiver essential oil, 2 to 4 parts of compound essential oil, 30 to 50 parts of natural soap base and the balance of cold-processed soap base, wherein the essential oil is metered in parts by volume. The invention has the beneficial effects of rich fragrance layers, lasting fragrance, rich product foam, fine and smooth hand feeling, good washing effect and good soap body stability. The product not only has a cleaning function, but also has the effects of inhibiting and killing bacteria, diminishing inflammation and resisting allergy, can effectively inhibit the formation of bacteria on the skin surface, can effectively relieve the problems of skin dryness, inflammation and the like, and can improve the skin state. In the mental level, the composition can effectively improve the mental state of a user, and has the effects of stimulating, resisting depression and relieving nervous mood.

The invention discloses use of preparations which takes cress total phenolic acid as a raw material medicine for preparing anti-human immunodeficiency virus drugs. The raw material medicine is extracted from the cress total phenolic acid, and the raw material medicine contains 55-80% of total phenolic acid. Through vitro experiments, the cress phenolic acid has strong anti-HIV activity against HIV; in vivo experiments, the cress total phenolic acid increases percentage of CD4+ T cells, increases the CD4+ / CD8+ value, and improves the immunosuppressive effects of the body to play an anti-virus role; the cress total phenolic acid has a good inhibitory effect on the activity of HIV reverse transcriptase. The cress total phenolic acid has the anti-HIV effect in vivo and in vitro, the effect isclear, the anti-HIV effect is achieved by inhibiting the activity of HIV reverse transcriptase, the mechanism of action is clear, and the potential for clinical use development is achieved.

The invention discloses an anti-H5N1 virus endocytosis antibody PTD-7B. The base sequence of the PTD-7B is shown as SEQ ID NO. 5 in a sequence table; the amino acid sequence of the fusion protein PTD-7B is shown as SEQ ID NO. 6 in a sequence table; the preparation method of the fusion protein PTD-7B comprises the following steps: 1) performing amplifying on a 7B gene of M1-ScFv by using a primer and using a screened phage antibody ScFv gene as a template; 2) connecting the amplified 7B gene into a PET28a-PTD-GFP vector, replacing a GFP gene fragment, and constructing a prokaryotic expression vector PET28a-PTD-7B; and 3) performing transforming on the prokaryotic expression vector into escherichia coli for expression and purification. The fusion protein PTD-7B is applied to preparation of anti-H5N1 human avian influenza virus drugs, and the result shows that the intracellular antibody has H5N1 virus neutralizing activity, and the titer is 350TCID50.

The invention relates to an application of lariciresinol-4-O-beta-D-glucoside in the preparation of drugs for treating or preventing flu and related inflammatory diseases caused by influenza virus. The application of lariciresinol-4-O-beta-D-glucoside in clinical treatment of influenza virus diseases has characteristics of safety, effectivity and little toxic or side effect. The invention also provides a pharmaceutical composition, an active ingredient of which comprises lariciresinol-4-O-beta-D-glucoside. By cytopathic-effect inhibition assay, it is verified that lariciresinol-4-O-beta-D-glucoside has an effect of inhibiting replication of influenza virus in the treatment mode. The result proves that lariciresinol-4-O-beta-D-glucoside has obvious effect of inhibiting influenza virus in the treatment mode. Selection index SI is greater than 4. The lariciresinol-4-O-beta-D-glucoside has advantages of low toxicity and high effectiveness.

The invention provides an infectious bovine rhinotracheitis virus (IBRV) aptamer. The aptamer has the maximum binding force and favorable specificity, can inhibit the virus infection on cells to some extent, and can be used for indirect immunofluorescent assay (IFA) after being marked by FAM. The aptamer can be used for inhibiting the virus infection on cells to perform the antivirus action, and can also be used as an anti-IBRV probe or target spot for preparing anti-IBRV drugs or reagents.

The invention discloses a H3N2 canine influenza virus shuttle intracellular antibody. The H3N2 virus is a type A influenza virus, and the main neutralizing antibody comes from the surface glycoprotein hemagglutinin (HA), so HA has become the main research target in the past; However, influenza virus is highly variable, and the immune cross-reaction between different variant branches is weak. M1 antibody can bind to M1 protein, inhibit its activity, interfere with the replication, transcription and release of influenza virus, and thus play an antiviral role. Therefore, we choose M1 protein to prepare the corresponding antibody to obtain a stable titer, couple it with TAT protein PTD, which can transduce biological macromolecules into cells, express the fusion protein TAT PTD‑M1 ScFv, and prepare anti- The shuttle antibody of canine influenza virus provides a new way for the treatment of canine influenza.

The invention discloses a H3N2 canine influenza virus shuttle intracellular antibody TAT‑4F. The H3N2 virus is a type A influenza virus, and the most important neutralizing antibody comes from the surface glycoprotein hemagglutinin (HA), so HA has become the main antibody in the past. Research targets; however, influenza viruses are highly variable, and the immune cross-reaction between different variant branches is weak. M1 antibodies can bind to M1 protein, inhibit its activity, and interfere with the replication, transcription and release of influenza viruses, thus playing a role To this end, we choose the M1 protein to prepare the corresponding antibody to obtain a stable titer, couple it with the TAT protein PTD, which can transduce biological macromolecules into cells, and express the fusion protein TAT PTD‑M1 ScFv, The shuttle antibody against canine influenza virus was prepared, which provided a new way for the treatment of canine influenza.

The invention provides Sargassum henslowianum fucosan sulfate which is composed of fucose and galactose and which has molecular weight of 4.0*10<5> to 6.0*10<5>. The Sargassum henslowianum fucosan sulfate is extracted from Sargassum henslowianum. By extracting Sargassum henslowianum crude polysaccharide and subjecting the crude polysaccharide to multiple times of purification with h ion exchange chromatography and molecular sieve chromatographic columns, the Sargassum henslowianum fucosan sulfate capable of being used for anti-herpes virus research is obtained. According to the scheme, the natural polysaccharide which has a novel antiviral action mode, is low in toxicity, efficient, safe and easy to accept can be separated from the marine medicinal resource Sargassum henslowianum, and a research basis can be provided for further developing antiviral drugs which have novel action mechanisms, definite curative effects and definite targets. The Sargassum henslowianum fucosan sulfate can play an antiviral role by effectively inhibiting herpes viruses from being adsorbed to the surface of host cells, and the acting site of the Sargassum henslowianum fucosan sulfate is the surface of thecells, can act on the first step of virus infection and does not enter the cells, so that the possibility of causing toxic and side effects is relatively small.

The invention relates to an external-use pure traditional Chinese medicine disinfectant formula containing ethyl alcohol. For the problems that the existing common disinfection measures are insufficient and health hazards to human bodies can be caused by long-time use, the external-use pure traditional Chinese medicine disinfectant formula containing ethyl alcohol, which can be directly used on the surface of skin and tableware disinfection measures, is researched and developed. Chinese herbal medicines in the formula in the invention have pharmacological efficacies of promoting the dispersingfunction of the lung, regulating vital energy, reducing phlegm, soothing, tranquilizing, preventing cold, caring skin, preventing oxidation and the like, and can be used for protection of various pathogens and viruses, in particular for sterilized using of transmission and infection of novel coronavirus. The disinfectant manufacturing process in the invention is simple and easy to do; the effectis obvious; and the disinfectant can be used as the necessary self-made bactericidal disinfectant in daily work life.

The invention discloses environmentally-friendly meat duck feed, which comprises the following raw materials in parts by weight: 40-60 parts of corns, 20-30 parts of soybean meal, 3-4 parts of rapeseed dregs, 0-11 parts of rice bran meal, 1-2 parts of mountain flour, 1-3 parts of dried folium mori, 2-5 parts of herba artemisiae annuae, 0-3 parts of dried cacumen platycladi, 0.5-2 parts of dried fructus crataegi, 1-3 parts of folium syringae, 1-2 parts of calcium hydrophosphate, 0.1-0.2 part of DL-methionine, 0-0.2 part of lysine, 0.3 part of vitamin and trace element premix and 0.5 part of composite additive premix. The corn, the soybean meal and the rapeseed dregs provide sufficient nutrition for ducks, the mountain flour and the calcium hydrophosphate perform a function of calcium supplementation, the dried folium mori, the herba artemisiae annuae and the dried cacumen platycladi perform a function of resisting bacteria, the dried fructus crataegi performs a function of inhibiting escherichia coli, pseudomonas aeruginosa and staphylococcus aureus, and the folium syringae performs a function of nourishing the stomach and increasing a digestion function. The environmentally-friendly meat duck feed can improve the immunity of ducks and reduce the utilization of antibiotics.

The invention discloses an anti-H5N1 viropexis antibody PTD (Protein transduction domain)-3F. The base sequence of the anti-H5N1 viropexis antibody PTD-3F is disclosed in SEQ ID NO. 5. The amino acidsequence of the fusion protein PTD-3F is disclosed in SEQ ID NO. 6. A preparation method for the fusion protein PTD-3F comprises the following steps of: 1) utilizing a primer, and taking a screened bacteriophage antibody ScFv gene as a template to carry out 3F gene amplification; 2) connecting an amplified 3F gene into a PET28a-PTD-GFP carrier to construct a prokaryotic expression vector PET28a-PTD-3F; and 3) converting the prokaryotic expression vector into escherichia coli to be expressed and purified. The invention discloses application of the PTD-3F for preparing a medicine capable of resisting H5N1 type human avian influenza viruses. A result displays that an intracellular antibody can neutralize H5N1 virus activity, and titer is 400 TCID50 (median tissue culture infective dose).

The invention belongs to the technical field of pig raising, solves the technical problems that in the prior art, pig feed is low in absorption and digestion rate, and pigs are prone to getting sick, and provides liquid-state fermented pig feed, a fermentation method and a conveying equipment. The liquid-state fermented pig feed comprises the following components: feed raw materials, water and a premix; the premix comprises the following raw materials in percentage by weight: 10%-20% of various vitamins, 10%-15% of lysine, 15%-20% of trace elements and 50%-60% of beneficial bacteria. The weight ratio of the feed raw materials to the premix is 19: 1; the ratio of a mixture composed of the feed raw materials and the premix to water is (1: 2)-(1: 3). The fermentation method of the liquid-state fermented pig feed has the advantages that the produced pig feed is good in absorption and digestion, and the health of pig herds is guaranteed.