Method for researching active ingredients of chrysanthemum for relieving dry eye symptoms

An active ingredient, chrysanthemum technology, applied in the research field of chrysanthemum active ingredients

Inactive Publication Date: 2018-06-26
北京京华医药有限公司
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Problems solved by technology

At present, there are reports on the chemical constituent...

Method used

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Embodiment Construction

[0010] Chrysanthemum's chemical constituents were identified by separation and structure identification using chromatography, Pope and other techniques, and the effective material basis of molybdenum chrysanthemum was determined, and monomer compounds were provided for research.

[0011] The human RPE cells cultured in vitro with the same cell culture termination time were irradiated with blue light of the same light intensity for the same time, and were divided into three groups. Group A: the nutrient solution of human RPE cells cultured in vitro contained molybdenum extract; Group B: in vitro The nutrient solution of cultured human RPE cells contains the monomer compound liquid isolated from molybdenum chrysanthemum; group C: the nutrient solution of human RPE cells cultured in vitro does not contain the above substances. Using terminal deoxynucleotidyl transferase-mediated in situ nick-end labeling (TUNEL), fluorescein-labeled annexin V / pyridine iodide (Annexin V-FITC / PI) do...

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Abstract

The invention relates to a method for researching active ingredients of chrysanthemum, in particular to a research on active ingredients of chrysanthemum for relieving dry eye symptoms. A chromatography technology and a spectrum technology and the like are adopted to qualitatively and quantitatively analyze mingmu chrysanthemum samples, and the molybdenum content of the mingmu molybdenum is determined by atomic fluorescence, atomic absorption, or ICP-MS (inductively coupled plasma mass spectrometry) and the like. Pharmacodynamic activity tracking is performed by establishing an optical damagein vitro efficacy evaluation model of blue light-induced human retinal pigment epithelial (HRPE) cells, the influence of mingmu chrysanthemum extract and a monomer compound on an oxidative stress state of the optical damage HRPE cells is observed, and the relieving effect of the mingmu chrysanthemum on the dry eye symptoms is evaluated.

Description

technical field [0001] The invention relates to a research method of active ingredients of chrysanthemum, in particular to a research on active ingredients of chrysanthemum for alleviating dry eyes. Background technique [0002] Chrysanthemum is a plant of Compositae. Chrysanthemum Chrysanthemum morifolium Ramat is a dried flower head of Chrysanthemum chrysanthemum, which is a commonly used traditional Chinese medicine in China. It mainly treats diseases such as wind-heat cold, headache, dizziness, red eyes, dysphoria in the heart and chest, furunculosis, and swelling. Modern pharmacological studies have shown that chrysanthemum has various pharmacological activities such as treating coronary heart disease, lowering blood pressure, preventing hyperlipidemia, antibacterial, antiviral, anti-inflammatory, and anti-aging. "Shen Nong's Materia Medica" records: white chrysanthemum "maintains dizziness, swelling and pain, loss of eyesight, dead skin muscles, aversion to rheumatis...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N21/64G01N21/31G01N27/62G01N33/50
CPCG01N21/3103G01N21/6404G01N27/62G01N30/02G01N33/5008
Inventor 程雪翔
Owner 北京京华医药有限公司
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