Real-time fluorescent quantitative PCR based quick and portable type molecular detecting equipment

Abstract

The invention discloses real-time fluorescent quantitative PCR based quick and portable type molecular detecting equipment, and belongs to the field of molecular biology and medical detecting apparatuses. The real-time fluorescent quantitative PCR based quick and portable type molecular detecting equipment comprises a micro real-time fluorescent quantitative PCR reaction system for placing a PCR reaction tube, a fluorescent signal sensing system for converting a real-time fluorescent signal in a PCR reaction process into an electric signal and sending to a central processing control system, the central processing control system for controlling each module to orderly work, a data communication system for sending the real-time fluorescent signal to an upper computer or a handheld terminal which is used for observing a fluorescent quantitative PCR reaction state in real time, and analyzing and judging PCR detection results. The real-time fluorescent quantitative PCR based quick and portable type molecular detecting equipment adopts the micro real-time fluorescent quantitative PCR reaction system and the corresponding sending, control and communication systems, so that portability of the apparatus is guaranteed; the upper computer or the handheld terminal is used for performing real-time data analysis, so that unnecessary resource and time waste is avoided, and equipment and experimental cost is effectively reduced.

Description

technical field [0001] The invention belongs to the technical field of molecular biology and medical detection instruments, in particular to a fast and portable molecular detection device based on real-time fluorescent quantitative PCR (Polymerase Chain Reaction, polymerase chain reaction). Background technique [0002] Biological information is a signal to regulate and control life activities. It is one of the three elements (matter, energy, and information) that constitute an organism, and plays an important role in the survival, reproduction, and metabolism of organisms. Biological information covers a wide range, and usually refers to DNA, RNA, etc. that contain genetic information in a narrow sense. These sequences with genetic information vary from person to person, and may be mutated by physical, chemical, biological and other factors, or errors may occur in the process of self-replication, and some replication, transcription errors or mutations will cause human body ...

AI Technical Summary

Problems solved by technology

[0005] Existing real-time fluorescence quantitative PCR instruments are mostly oriented to laboratories and large and medium-sized medical institutions. They are bulky, heavy and inconvenient to carry, and the operation is complicated. Professional experimenters are required to operate, and the average test time is more than 2 hours, so they are not fast. On-site real-time detection and diagnosis requirements such as analysis, miniaturization, and portability

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