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A human nk/t cell line

A cell line and deposit number technology, applied in the field of cell lines, can solve the problems of cancer patients without T-cell NK cells, the difficulty of purifying NK cells and T cells, the difficulty of mass expansion in vitro, and the time-consuming adoptive immunotherapy.

Active Publication Date: 2020-11-06
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, adoptive immunotherapy is time-consuming, and cancer patients may not have enough T cells or NK cells available
At the same time, there are certain difficulties in the purification and large-scale expansion of NK cells and T cells in vitro

Method used

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  • A human nk/t cell line
  • A human nk/t cell line
  • A human nk/t cell line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1. Establishment of L311 cell line

[0031] 1.1 The source of the specimen:

[0032] 2ml of peripheral blood was taken from a female patient with chronic active EBV infection, anticoagulated with heparin. The informed consent was signed with the consent of the patients and their families.

[0033] 1.2 Isolation of peripheral blood mononuclear cells:

[0034] Aseptically extract 2ml of peripheral blood from the patient, and conduct density gradient centrifugation with lymphocyte separation medium at 2000 rpm for 20 minutes at a room temperature of 20°C. Aspirate the mononuclear cells in the middle layer, wash twice with PBS, 1500 rpm, 10 minutes, room temperature 20 degrees. Add fresh and complete 1640 culture medium, divide 2ml of cell suspension into wells of a 24-well culture plate, and store at 37°C, 5% CO 2 Culture in an incubator, and add 50-700 U of recombinant human IL-2.

[0035] 1.3 Cell culture and establishment

[0036] After the cells were cultured for...

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Abstract

The invention relates to a cell line, and specifically relates to a NK / T cell line of the human. The conservation number is CGMCC No. 14892. The NK / T cell line of the human provided by the invention can be passaged for a long time and greatly amplified, and has stronger anti-tumor activity, and provides a new experimental model for deeply development chronic activity EBV infectious disease mechanism research, screening chronic activity EBV infection, diagnosis molecular marker of EBV related lymphocytosis and the like, treating prognosis maker and treatment medicine, CD8+T cell, natural killercell anti-tumor action mechanism and clinic adoptive immunity treatment for a researcher.

Description

technical field [0001] The present invention relates to cell lines, in particular to a human NK / T cell line. Background technique [0002] T cells can be divided into two subgroups according to whether they express CD4 or CD8 receptor molecules. CD8+ T cells are mainly responsible for the elimination of target cells through direct killing, such as cells infected by viruses and cells expressing tumor-specific antigens. Natural killer cells (NK cells) are important immune cells in the body, which have anti-tumor, anti-viral infection and immune regulation functions. [0003] NK cells and T cells have long been considered excellent weapons for killing cancer cells and treating infections. Adoptive immunotherapy typically involves harvesting T cells from cancer patients, arming them with tumor-specific receptors in the laboratory, and reinfusing them back into the patient. However, adoptive immunotherapy is time-consuming, and cancer patients may not have enough T cells or NK...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/0783C12Q1/02A61K35/17A61P35/00
CPCA61K35/17C12N5/0636C12N5/0646G01N33/5011
Inventor 段子渊许崇凤谢正德张庆勋
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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