Single-carrier low-temperature-resistant polycyclic aromatic hydrocarbon mixed bacterial agent and its preparation method and application
A technology of mixing bacterial agents and polycyclic aromatic hydrocarbons, which is applied in the direction of biochemical equipment and methods, fixed on/in organic carriers, and methods based on microorganisms, which can solve the problem of large differences between application results and experimental results, and unreasonable Remediation of contaminated soil and other issues, to achieve the effect of uniform internal structure, low cost, and simple preparation process
Active Publication Date: 2021-06-18
宁夏荟峰农副产品有限公司
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Problems solved by technology
Some studies have shown that the free microorganisms added to the remediation site may be due to the vicious competition of indigenous bacteria or the difficulty in adapting to the environment, resulting in a large difference between the application results and the experimental results, making it unsuitable for in-situ remediation of contaminated soil.
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Embodiment
[0035] Embodiment A method for degrading polycyclic aromatic hydrocarbon pollutants in freeze-thaw soil
[0036] (1) Culture of Pseudomonas sp. SDR4
[0037] Complete medium: 5g beef extract, 10g peptone, 10g glucose, 5g yeast powder, 5g NaCl, 20g agar, 1000mL distilled water, pH 7.1-7.2, sterilized at 121°C for 20min.
[0038] Get the Pseudomonas sp. (Pseudomonassp.) SDR4 preserved on the slant culture medium with the preservation number of CGMCC NO.14048, inoculate it in the complete medium at an inoculum size of 5%, and culture it on a shaker at 15° C. for 30 minutes at 120 r / min. ~40h, until the bacterial density is 6×10 8 CFU mL －1 , to get Pseudomonas (Pseudomonas sp.) SDR4 bacteria suspension.
[0039] (2) Cultivation of Mortierella alpine JDR7
[0040] Complete medium: 5g beef extract, 10g peptone, 10g glucose, 5g yeast powder, 5g NaCl, 20g agar, 1000mL distilled water, pH 7.1-7.2, sterilized at 121°C for 20min.
[0041] Get the Mortierella alpine (Mortierella alp...
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Abstract
The invention discloses a single-carrier low-temperature-resistant polycyclic aromatic hydrocarbon mixed bacterial agent, a preparation method and application thereof. It consists of a mixed bacterial agent obtained by mixing Pseudomonas SDR4 and Mortierella alpina JDR7 screened in freeze-thawed soil at a volume ratio of 1:1 and corncobs as a carrier. The single-carrier low-temperature-degradable polycyclic aromatic hydrocarbon mixed bacterial agent of the present invention has good biological activity, low cost, simple preparation process, does not introduce secondary pollution, does not need to be recycled, and can effectively shield indigenous bacteria, phages and toxic substances from affecting microorganisms. Vicious competition, phagocytosis and poisoning make it stable and high performance in complex environments. The invention has obvious remediation effect on freeze-thaw soil polluted by polycyclic aromatic hydrocarbons in cold regions.
Description
technical field [0001] The invention relates to a microorganism immobilization technology, in particular to using corncobs as a carrier and adopting an adsorption method to immobilize low-temperature resistant mixed bacteria to degrade polycyclic aromatic hydrocarbons in freeze-thaw soil in cold regions. [0002] The Pseudomonas sp. SDR4 referred to in the present invention has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on April 19, 2017, and the preservation number is CGMCC NO.14048. [0003] Mortierella alpine (Mortierella alpine) JDR7 referred to in the present invention has been preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee on January 5, 2018, and the preservation number is CGMCC NO.15183. Background technique [0004] Polycyclic aromatic hydrocarbons (Polycyclic aromatic hydrocarbons, PAHs) are a class of organic compounds composed of...
Claims
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IPC IPC(8): C12N11/02B09C1/10A62D3/02C12R1/38C12R1/645A62D101/20
CPCA62D3/02A62D2101/20B09C1/10B09C1/105C12N11/02
Inventor 苏丹巩春娟陈忠林普聿
Owner 宁夏荟峰农副产品有限公司