A method for detecting microorganisms based on silver nanoparticles functionalized with phenylboronic acid
A technology of silver nanoparticles and mercaptophenylboronic acid, applied in the field of analytical chemistry, can solve the problem of no colorimetric method, etc., and achieve the effects of simple operation, simple process, and great popularization and use value.
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Embodiment 1
[0023] Preparation of silver nanoparticles functionalized with phenylboronic acid
[0024] Add 1 mL of 100 mM silver nitrate solution and 1 mL of 100 mM trisodium citrate solution into 36.8 mL of ultrapure water under vigorous stirring. Then, 1.2 mL of freshly prepared 100 mM sodium borohydride was added. The color of the solution will gradually turn yellow. After about 10 minutes of reaction, it is aged at 4°C for 2 days. Take 1 mL of prepared silver nanoparticles and dilute them 16 times, then add 10 μL of 4-mercaptophenylboronic acid, and react for 30 minutes to obtain silver nanoparticles functionalized with phenylboronic acid (see figure 1 ).
[0025] Such as figure 1 Shown, a is the ultraviolet-visible absorption spectrogram of the silver nanoparticle of phenylboronic acid functionalization, and it has a sharp absorption peak at 398nm place; 7 cfu mL -1 coli, its absorbance at 398nm decreased slightly, but no other peaks appeared; c is the UV-vis absorption spectrum...
Embodiment 2
[0027] Detection of Escherichia coli Based on UV-Vis Absorption Spectroscopy
[0028] Add 100 μL of Escherichia coli to 1 mL of silver nanoparticles functionalized with phenylboronic acid. After 10 minutes of reaction, add 10 μL of 300 μM mercaptophenylboronic acid. scan (see figure 2 ),Depend on figure 2 It can be seen that as the concentration of E. coli increases, its maximum absorbance value at 398nm increases gradually. The logarithm value of its absorbance value and E. coli concentration is 5×10 4 cfu mL -1 to 1×10 7 cfu mL -1 linear relationship over the concentration range (see image 3 ).
Embodiment 3
[0030] Visual detection of E. coli
[0031]Add 100 μL of Escherichia coli to 1 mL of silver nanoparticles functionalized with phenylboronic acid, react for 10 minutes, add 10 μL of 3 mM mercaptophenylboronic acid, react for 1 minute, and observe the color of the solution (see Figure 4 ). Depend on Figure 4 As shown, as the concentration of Escherichia coli decreased, its color showed a changing trend from light yellow to light red. The minimum detection limit that can be distinguished by the naked eye is 5×10 5 cfu mL -1 .
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