Quality control method of traumatology department yellow-water preparation

Abstract

The invention provides a quality control method of a traumatology department yellow-water preparation. The method comprises the following steps of (1) weighing phellodendrine chloride, jatrorrhizine hydrochloride, epiberberine chloride, coptisine chloride, palmatine chloride, berberine hydrochloride, geniposide and chlorogenic acid; adding absolute methanol or methanol water solution for preparinga reference product solution; (2) taking contents of the traumatology department yellow-water preparation to be tested; adding absolute methanol or methanol water solution for dissolution; performingfiltering; taking subsequent filtering liquid to be as a solution to be tested; (3) respectively taking the reference product solution and 1 to 5 muL of the solution to be tested; injecting the materials into a liquid chromatography-mass spectrometry instrument for detection; obtaining the contents of phellodendrine, jateorhizine, epiberberine, coptisine, palmatine, berberine, geniposide and chlorogenic acid through calculating a quantitative chromatogram map. The method has the advantages that the original deficient quantitative evaluation index can be completed by once quantitative determination. The quantitative chromatogram map of the method can show good wave peak separation; the identification by the method is fast and accurate.

Description

technical field [0001] The invention relates to the technical field of quality control, in particular to a quality control method for traumatic yellow water preparations. Background technique [0002] Traumatology Huangshui is a product developed by the applicant, and it obtained a related patent in 2000 (patent announcement number: 96123078.9). However, because it is a clinical prescription, the research work on the quality evaluation of this variety has not been carried out in depth, resulting in the production of preparations. The process is relatively extensive, the quality standard is low, and the clinical efficacy is difficult to control. It is particularly worth noting that the compound composition of this preparation is complex and its material basis is unknown. In terms of quality standards, it is still based on appearance, sensory judgment and qualitative identification (thin-layer chromatography), and there is still a lack of well-characterized index components. ...

AI Technical Summary

Problems solved by technology

As a result, a total of 20 ingredients in the compound were found and confirmed, and there are still a large number of substances whose parent ion molecular weight can match, but due to the unsatisfactory chromatogram, they were not included in the results

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