Anti-wrinkle moisturizing moisture retention essence and preparation method thereof
An essence and water-locking technology, applied in the field of essence, can solve the problems of hindering the penetration of skin care products, the loss of natural moisturizing factors, impurities and metabolites that cannot be eliminated in time, and achieve the promotion of young cell regeneration, good anti-wrinkle moisturizing effect, The effect of delaying skin aging
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[0054] The preparation method of papain silk fibroin polypeptide is as follows:
[0055] S1 degumming: put the silk into 1.0% sodium carbonate aqueous solution with a mass fraction of 1.0% and boil it for 50 minutes for degumming, take out the silk, wash it with water, and dry it to obtain silk fibroin;
[0056] S2 Dissolution and impurity removal: Add the dried silk fibroin into 40% calcium chloride aqueous solution at a bath ratio of 1:10g / mL, boil until the silk fibroin is completely dissolved, cool to room temperature, vacuum filter, and the obtained filtrate is packed Put it into a dialysis bag and dialyze with distilled water for 3 days to obtain a silk fibroin solution, and concentrate it to the volume of the original filtrate by rotary evaporation to obtain a silk fibroin solution;
[0057] S3 Add papain to silk fibroin solution at 100:2mL / g, enzymatically hydrolyze for 5h at a temperature of 55°C and a pH of 7, then heat to 100°C to inactivate the enzyme for 20min, th...
Embodiment 1
[0061] An anti-wrinkle, moisturizing and water-locking essence, prepared from the following raw materials by mass percentage: 6% white fungus extract, 6% papain silk fibroin polypeptide, 5% seaweed extract, 1% sodium hyaluronate, 2% Yunzhi extract, 2% natto extract, 1% lavender extract, 1% thyme extract, 1% helichrysum extract, and the balance is water.
[0062] A preparation method of an anti-wrinkle, moisturizing and water-locking essence: weigh the raw materials according to the formula, and stir the raw materials for 30 minutes at 25°C and 300 rpm.
Embodiment 2
[0064] An anti-wrinkle, moisturizing and water-locking essence, prepared from the following raw materials by mass percentage: 6% white fungus extract, 6% papain silk fibroin polypeptide, 5% seaweed extract, 1% sodium hyaluronate, 2% Yunzhi extract, 2% natto extract, 1% lavender extract, 1% thyme extract, 1% helichrysum extract, 0.5% chitosan-nanometer silver colloid solution, and the balance is water.
[0065] The preparation method of described chitosan-nanometer silver colloid solution is as follows: chitosan will be added to water and boiled 20min by mass ratio 2:100, filter, obtain filtrate; Molar concentration is the silver nitrate aqueous solution boiling of 1mmol / L, Add above-mentioned filtrate dropwise with the speed of 0.05g / s, after dropwise, remove heat source, be cooled to room temperature and obtain chitosan-nanometer silver colloid solution; The volume ratio of described filtrate and silver nitrate aqueous solution is 9:50.
[0066] A preparation method of an ant...
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