A kind of slow and controlled oxygen release microsphere and its preparation method and application

Abstract

The invention discloses a sustained-release oxygen microsphere as well as a preparation method and application thereof. The sustained-release oxygen microsphere is a microsphere using gelatin as a matrix; the interior of the microsphere contains peroxidated inorganic particles, and preferably, the peroxidated inorganic particles are selected from one or multiple of CaO2 (calcium peroxide), MgO2 (magnesium peroxide), NaCO3 (sodium carbonate), SrO2 (strontium peroxide), BaO2 (barium peroxide), K2O2 (potassium peroxide), and Na2O2 (sodium peroxide). The sustained-release oxygen microsphere has the advantages that the size is controllable, the oxygen release time is long and controllable, the sudden release rate is low, the cell toxicity is low, the immunogenicity is low, the preparation process is relatively simple, the industrialized production is easily realized, and the like; the larger application prospect is realized in clinical application of bone defect repair.

Description

technical field

[0001] The invention belongs to the field of preparation of bone engineering artificial slow-release materials, in particular to a gelatin composite CaO 2 The preparation method of the constructed controllable oxygen release type tissue engineering material. Background technique

[0002] As we all know, some small bone defects in the human body can be repaired by themselves, but large bone defects caused by bone tumor resection, traumatic massive bone loss, osteonecrosis, bone metabolic diseases, etc. cannot be repaired by themselves and require human intervention. At present, large bone defects can be repaired by bone tissue engineering and bone grafting. Among them, bone transplantation includes autograft, allograft, and xenograft, but they all have their own obvious disadvantages, such as the possibility of disease transmission, lack of tissue that can be used for autologous transplantation, and donors with high disease risk. risk etc. In recent years, ...

Examples

Embodiment 1

[0083] Example 1 Preparation of oxygen-releasing microspheres

[0084] 1) Preparation of gelatin solution: prepare a gelatin solution with a concentration of 0.8g / ml, the specific method is as follows:

[0085] ①Weigh 8g of gelatin into a beaker, then add 10ml of PBS, place on a magnetic stirrer and stir at 500rpm for about 5min;

[0086] ②Seal ① with a disposable sterile sealing film, place at 54°C to fully dissolve the gelatin in PBS (50min), and set aside;

[0087] Preparation of nanofiber film: prepare a hexafluoroisopropanol solution of collagen powder, and make the solution into a nanofiber film for in vivo experiments;

[0088] 2) Preparation of liquid paraffin dispersed phase: the specific method is as follows:

[0089] ①Preheat the liquid paraffin at 54°C for 1 hour;

[0090] ② Take 100ml into a 250ml beaker, put it on a heating magnetic stirrer (54°C), stir at 1500rpm, add 1ml of emulsifier Span80 at the same time, keep stirring, and fully emulsify for 20min;

[...

Embodiment 2

[0101] Example 2 Preparation of oxygen-releasing microspheres

[0102] 1) Preparation of gelatin solution: prepare a gelatin solution with a concentration of 0.8g / ml, the specific method is as follows:

[0103] ①Weigh 8g of gelatin into a beaker, then add 10ml of PBS, place on a magnetic stirrer and stir at 500rpm for about 5min;

[0104] ②Seal ① with a disposable sterile sealing film, place at 54°C to fully dissolve the gelatin in PBS (50min), and set aside;

[0105] Preparation of nanofiber film: prepare a hexafluoroisopropanol solution of collagen powder, and make the solution into a nanofiber film for in vivo experiments;

[0106] 2) Preparation of liquid paraffin dispersed phase: the specific method is as follows:

[0107] ①Preheat the liquid paraffin at 54°C for 1 hour;

[0108] ② Take 100ml into a 250ml beaker, place it on a heating magnetic stirrer (54°C), stir at 1000rpm, add 1ml emulsifier Span80 at the same time, keep stirring, and fully emulsify for 20min;

[0...

Embodiment 3

[0119] Example 3 Preparation of oxygen-releasing microspheres

[0120] 1) Preparation of gelatin solution: prepare a gelatin solution with a concentration of 0.8g / ml, the specific method is as follows:

[0121] ①Weigh 8g of gelatin into a beaker, then add 10ml of PBS, place on a magnetic stirrer and stir at 500rpm for about 5min;

[0122] ②Seal ① with a disposable sterile sealing film, place at 54°C to fully dissolve the gelatin in PBS (50min), and set aside;

[0123] Preparation of nanofiber film: prepare a hexafluoroisopropanol solution of collagen powder, and make the solution into a nanofiber film for in vivo experiments;

[0124] 2) Preparation of liquid paraffin dispersed phase: the specific method is as follows:

[0125] ①Preheat the liquid paraffin at 54°C for 1 hour;

[0126] ② Take 100ml into a 250ml beaker, place it on a heating magnetic stirrer (54°C), stir at 500rpm, add 1ml of emulsifier Span80 at the same time, keep stirring, and fully emulsify for 20min;

...

technical field

[0001] The invention belongs to the field of preparation of bone engineering artificial slow-release materials, in particular to a gelatin composite CaO 2 The preparation method of the constructed controllable oxygen release type tissue engineering material. Background technique

[0002] As we all know, some small bone defects in the human body can be repaired by themselves, but large bone defects caused by bone tumor resection, traumatic massive bone loss, osteonecrosis, bone metabolic diseases, etc. cannot be repaired by themselves and require human intervention. At present, large bone defects can be repaired by bone tissue engineering and bone grafting. Among them, bone transplantation includes autograft, allograft, and xenograft, but they all have their own obvious disadvantages, such as the possibility of disease transmission, lack of tissue that can be used for autologous transplantation, and donors with high disease risk. risk etc. In recent years, ...

Examples

Embodiment 1

[0083] Example 1 Preparation of oxygen-releasing microspheres

[0084] 1) Preparation of gelatin solution: prepare a gelatin solution with a concentration of 0.8g / ml, the specific method is as follows:

[0085] ①Weigh 8g of gelatin into a beaker, then add 10ml of PBS, place on a magnetic stirrer and stir at 500rpm for about 5min;

[0086] ②Seal ① with a disposable sterile sealing film, place at 54°C to fully dissolve the gelatin in PBS (50min), and set aside;

[0087] Preparation of nanofiber film: prepare a hexafluoroisopropanol solution of collagen powder, and make the solution into a nanofiber film for in vivo experiments;

[0088] 2) Preparation of liquid paraffin dispersed phase: the specific method is as follows:

[0089] ①Preheat the liquid paraffin at 54°C for 1 hour;

[0090] ② Take 100ml into a 250ml beaker, put it on a heating magnetic stirrer (54°C), stir at 1500rpm, add 1ml of emulsifier Span80 at the same time, keep stirring, and fully emulsify for 20min;

[...

Embodiment 2

[0101] Example 2 Preparation of oxygen-releasing microspheres

[0102] 1) Preparation of gelatin solution: prepare a gelatin solution with a concentration of 0.8g / ml, the specific method is as follows:

[0103] ①Weigh 8g of gelatin into a beaker, then add 10ml of PBS, place on a magnetic stirrer and stir at 500rpm for about 5min;

[0104] ②Seal ① with a disposable sterile sealing film, place at 54°C to fully dissolve the gelatin in PBS (50min), and set aside;

[0105] Preparation of nanofiber film: prepare a hexafluoroisopropanol solution of collagen powder, and make the solution into a nanofiber film for in vivo experiments;

[0106] 2) Preparation of liquid paraffin dispersed phase: the specific method is as follows:

[0107] ①Preheat the liquid paraffin at 54°C for 1 hour;

[0108] ② Take 100ml into a 250ml beaker, place it on a heating magnetic stirrer (54°C), stir at 1000rpm, add 1ml emulsifier Span80 at the same time, keep stirring, and fully emulsify for 20min;

[0...

Embodiment 3

[0119] Example 3 Preparation of oxygen-releasing microspheres

[0120] 1) Preparation of gelatin solution: prepare a gelatin solution with a concentration of 0.8g / ml, the specific method is as follows:

[0121] ①Weigh 8g of gelatin into a beaker, then add 10ml of PBS, place on a magnetic stirrer and stir at 500rpm for about 5min;

[0122] ②Seal ① with a disposable sterile sealing film, place at 54°C to fully dissolve the gelatin in PBS (50min), and set aside;

[0123] Preparation of nanofiber film: prepare a hexafluoroisopropanol solution of collagen powder, and make the solution into a nanofiber film for in vivo experiments;

[0124] 2) Preparation of liquid paraffin dispersed phase: the specific method is as follows:

[0125] ①Preheat the liquid paraffin at 54°C for 1 hour;

[0126] ② Take 100ml into a 250ml beaker, place it on a heating magnetic stirrer (54°C), stir at 500rpm, add 1ml of emulsifier Span80 at the same time, keep stirring, and fully emulsify for 20min;

...

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