Method for repairing heavy metal-polluted saline alkali soil by combining marine yeast and plants

A phytoremediation and saline-alkali soil technology, applied in the field of heavily polluted soil remediation, can solve the problems of single use method, few varieties, and poor remediation effect, and achieve easy management, no secondary pollution, and improved plant enrichment. Effect

Active Publication Date: 2018-07-24
WENZHOU MEDICAL UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, the existing biosurfactants have just started with few varieties, the repair effect is not good, and the use method is relatively single

Method used

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  • Method for repairing heavy metal-polluted saline alkali soil by combining marine yeast and plants
  • Method for repairing heavy metal-polluted saline alkali soil by combining marine yeast and plants
  • Method for repairing heavy metal-polluted saline alkali soil by combining marine yeast and plants

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Embodiment 1

[0034] 1) Strain isolation and screening:

[0035] The collected seawater samples and sterile water-treated sediment concentrate were enriched and cultured in sterilized artificial seawater culture medium (MMC) at 28°C and 200 r / min. Screening of the produced surfactant microorganisms can be determined by ①Degradation rate of diesel: Degradation rate=(Blank diesel concentration-Diesel concentration in culture solution) / Blank diesel concentration×100% ②Droplet collapse test: Take 5μl to remove bacteria The fermentation broth was dropped into a 96-well plate covered with oil film, and the shape of the drop was observed with a magnifying glass. ③Immune hemolysis experiment: Spot the microbial colony on the blood plate, observe whether there is a transparent hemolysis circle around a single colony, and compare the diameter of the hemolysis circle ④Determination of the size of the oil discharge ring: take a certain amount of fermentation broth from which the bacteria are removed by ce...

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Abstract

The invention provides a method for repairing heavy metal-polluted saline alkali soil by combining marine yeast and plants. The method comprises the following steps: (1) firstly obtaining a marine yeast strain; (2) fermenting the marine yeast strain to obtain a bio-surfactant fermented liquid; (3) planting stemless leaflet ficus microcarpa seeds into the polluted soil, adding the bio-surfactant fermented liquid into the polluted soil to repair the polluted soil together with stemless leaflet ficus microcarpa, wherein the marine yeast strain is mairine yarrowia lipolytica. According to the method provided by the invention, shortcomings such as easy retention of a chemical enhancer, difficulty in degradation and biotoxicity are avoided; due to use of solubilization and flow increasing effects of a bio-surfactant, the content of available heavy metals in the soil is increased, so that plant absorption is facilitated; repairing by the plants for a heavy metal-polluted land is enhanced; themethod is favorable for development of marine resources and has a wide application prospect.

Description

Technical field [0001] The invention relates to the technical field of remediation of heavily polluted soil, in particular to a method for remediating heavy metal contaminated saline-alkali soil with marine yeast combined with phytoremediation. Background technique [0002] Under the influence of man-made and natural effects, the phenomenon that heavy metals in the soil are higher than the original content and cause the deterioration of the ecological environment is becoming more and more common. As heavy metals can only undergo form transformation and migration in the soil, they are difficult to degrade, and the toxicity caused by different forms is different, so they are easily latent for a long time, produce biological amplification, and cause non-negligible harm to plants and humans. Therefore, using the anionic (negatively charged) nature of the biosurfactant to repair heavy metals in the soil, it can be adsorbed to the soil and combined with heavy metal ions, so that it can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/00B09C1/10
CPCB09C1/105B09C2101/00
Inventor 葛利云邓欢欢李少君马斌斌钟铭晨鲍根莲彭路菊马恒轶
Owner WENZHOU MEDICAL UNIV
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