Nucleic acid preserving fluid, preparation method thereof and method for applying nucleic acid preserving fluid

A technology for preservation solution and nucleic acid, applied in the field of nucleic acid preservation, which can solve the problems of high production cost, cumbersome operation, and heavy workload of preservation solution, and achieve the effects of low cost, convenient preparation process and low production cost

Inactive Publication Date: 2018-07-24
杭州百殷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the number of components used in the prior art is very large, which easily leads to high production costs of the preservation solution; and in the preparation process, when preparing a large number of components, it is easy to have disadvantages such as heavy workload and cumbersome operation.

Method used

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  • Nucleic acid preserving fluid, preparation method thereof and method for applying nucleic acid preserving fluid
  • Nucleic acid preserving fluid, preparation method thereof and method for applying nucleic acid preserving fluid

Examples

Experimental program
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Effect test

Embodiment 1

[0050] Example 1: Nucleic acid preservation solution, the components included and their corresponding dosages are shown in Table 1, and are prepared through the following steps:

[0051] Step 1, thoroughly mix trisodium citrate, biological buffer, disodium edetate and 30ml of pure water until completely dissolved to form the first mixture;

[0052] Step 2, add an acid-base regulator to the first mixture obtained in step 1, mix well, adjust the pH value to 3.5-4.0, then add preservative Proclin-300, and finally add pure water to 100ml to obtain nucleic acid preservation liquid.

[0053] Wherein, the concentration of triethanolamine hydrochloride is 400mmol / L, the concentration of N,N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid is 600mmol / L; the concentration of glacial acetic acid is 0.7mol / L, polyacrylic acid The concentration is 5wt%. In terms of bases, the length of a nucleic acid is 10,000 bases.

[0054] In addition, trisodium citrate, triethanolamine hydrochloride, ...

Embodiment 2-4

[0057] Example 2-4: Nucleic acid preservation solution, the difference from Example 1 is that the components included and their corresponding dosages are shown in Table 1.

Embodiment 5

[0058] Embodiment 5: Nucleic acid preservation solution, the difference from Example 1 is that in the biological buffer, the concentration of triethanolamine hydrochloride is 500mmol / L, N, N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid The concentration is 500mmol / L.

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Abstract

The invention discloses nucleic acid preserving fluid, a preparation method thereof and a method for applying the nucleic acid preserving fluid, and belongs to the technical field of nucleic acid preservation. The nucleic acid preserving fluid comprises the following components based on 100 mL preserving fluid: 5.8 to 5.95g of trisodium citrate, 19.56 to 20.21g of a biological buffer, 0.173 to 0.192g of ethylenediamine tetraacetic acid disodium, 8.2 to 9.0 ml of an acidity regulator, 28 to 33 [mu]L of a preservative, and the balance of purified water; the pH value of the preserving fluid is 3.5 to 4.0; the buffer comprises at least one of triethanolamine hydrochloride and N,N-di-(2-ethoxyl)-2-aminoethanesulfonic acid; the acidity regulator comprises at least one of glacial acetic acid andpolyacrylic acid. The preserving fluid has the advantage that the tissue structure of nucleic acid is kept stable at normal temperature.

Description

technical field [0001] The invention relates to the technical field of nucleic acid preservation, more specifically, it relates to a nucleic acid preservation solution, a preparation method thereof, and a method for preserving nucleic acid using the preservation solution. Background technique [0002] Nucleic acid is a biomacromolecular compound synthesized by many nucleotides. It exists widely in all animal and plant cells and microorganisms. Nucleic acid in organisms is often combined with protein to form nucleoprotein. According to different chemical composition, nucleic acid can be divided into ribonucleic acid (referred to as RNA) and deoxyribonucleic acid (abbreviated as DNA). [0003] The properties of nucleic acids are unstable and easily denatured. Therefore, during the research process, nucleic acids are usually stored at ultra-low temperatures (-70°C) for long-term storage. However, this storage method is likely to affect the structure of nucleic acids, and during...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
CPCC12N15/1003
Inventor 许安庆汪鹏刘恒生
Owner 杭州百殷生物科技有限公司
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