The invention provides automatic medium-pressure preparation and separation equipment, an automatic medium-pressure preparation and
separation method and an automatic separation and preparation
system. The automatic medium-pressure preparation and separation equipment comprises a
workbench; the sample loading, liquid adding and needle cleaning module, the column packing module, the fraction collecting module, the carrying mechanism and the pump valve module are arranged on the
workbench; the mobile phase cache module, the sample loading liquid adding and needle cleaning module which are arranged independent of the
workbench are used for sucking sample liquid and cleaning a sample loading needle; chromatographic columns and / or sample loading columns are installed on the column loading module through a carrying mechanism, samples are separated, and the chromatographic columns and / or the sample loading columns of various specifications can be arranged in the column loading module; the fraction collection module is used for collecting fractions; the carrying mechanism is used for transferring materials on the sample loading, liquid adding and needle cleaning module, the column packing module and the fraction collecting module; the mobile phase cache module caches the required mobile phase; and the pump valve module is respectively communicated with the sample loading, liquid adding and needle cleaning module, the column packing module, the fraction collecting module and the mobile phase caching module and is used for providing power for liquid transmission among the modules. According to the invention,
automation and unmanned operation of the whole process of medium-pressure preparation and separation are realized.