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Visible quantitative detection new method for heavy metal ions

A detection method, a metal technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of poor sensitivity and selectivity, cumbersome operation steps, pollution caused by organic solvent environment, etc., and achieve simple and efficient detection and analysis, specific effects

Inactive Publication Date: 2018-07-24
CHINA AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the traditional method for colorimetric detection of mercury still has certain limitations, such as cumbersome operation steps, poor sensitivity and selectivity, and the need to use organic solvents that easily pollute the environment. A highly sensitive and specific method to meet the needs of trace metal mercury detection

Method used

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  • Visible quantitative detection new method for heavy metal ions
  • Visible quantitative detection new method for heavy metal ions
  • Visible quantitative detection new method for heavy metal ions

Examples

Experimental program
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Embodiment 1

[0117] Embodiment 1, a method for detecting heavy metal Hg 2+ Establishment of a new method for ultrafast PCR-based colorimetric sensing

[0118] (1) Experimental materials

[0119] The nucleotide sequences of the primers designed in this example are shown in Table 1 and the sequence listing.

[0120] Table 1

[0121]

[0122] In Table 1, the nucleotide sequence on the left side of the connecting arm (oxyethyleneglycol bridge) of the upstream primer Primer 1 is the nucleotide sequence shown in SEQ ID No. 1 in the sequence table, and the nucleotide sequence on the right side of the connecting arm is the sequence The nucleotide sequence shown in SEQID №: 2 in the list, the chemical structure of the connecting arm is:

[0123]

[0124] In Table 1, the downstream primer Primer 2 is obtained after the first 5' nucleotide of the nucleotide shown in SEQ ID No. 3 in the sequence listing is labeled with biotin.

[0125] In Table 1, the nucleotide sequence of the template Temp...

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Abstract

The invention provides an ultrafast PCR-based colorimetric sensing new method used for visible ultrasensitive detection of Hg<2+>. According to the method, an amplification primer and a template of anultrafast Polymerase Chain Reaction (PCR) are designed according to mercury ion thymine mismatch, a color development module is self-assembled through the primer by combining enzyme-linked nucleic acid, so that a new mismatch-based mercury ion target function nucleic acid detection method and a biological sensor are created. The detection method and the biological sensor are faster and more sensitive than traditional methods, have characteristics of strong specificity, high sensitivity, and reliable detection result, simplify analysis and detection steps, shorten analysis time, moreover, makeonline real-time detection possible, and are portable and suitable for field work, thereby having a bright application prospect in the field of heavy metal rapid detection.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a method for detecting heavy metals and a biological sensor. Background technique [0002] Mercury rarely exists in the state of pure metal in nature, and usually exists in mineral deposits in the form of mercuric sulfide, chlorthimerite, and thionite. Mercury is a highly toxic heavy metal element. With the continuous acceleration of industrialization, more and more mercury is accumulated through the food chain, which is more harmful to the human body. It immerses into the human body through water, air, food, medicine, cosmetics and other channels, causing harm to the human body. Especially pregnant women should pay special attention to avoid exposure to mercury, because both organic mercury (methylated mercury) and inorganic mercury will have an impact on the health of the fetus. [0003] Traditional Hg 2+ Detection methods mainly include atomic absor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/686
CPCC12Q1/686C12Q2525/301
Inventor 许文涛罗云波黄昆仑杜再慧田晶晶
Owner CHINA AGRI UNIV
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