Characteristic sequence, primers and identification method of Carya illinoinensis varieties Nacono and Sumner

A thin-shelled pecan, characteristic sequence technology, applied in biochemical equipment and methods, microbial determination/inspection, recombinant DNA technology, etc., can solve the problems of low yield, instability, cumbersome detection, etc. Sex-enhancing effect

Active Publication Date: 2018-07-27
ZHEJIANG FORESTRY ACAD
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  • Abstract
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  • Claims
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Problems solved by technology

[0003] However, the main problem faced by the existing pecan production areas in my country is low yield and instability. There are many reasons for this phenomenon, one of which is the lack of clear genetic relationship analysis of the existing introduced varieties Coupled with the confusion in the naming of some hybrid offspring, it is difficult to carry out effective parent selection and rational allocation, and it is not convenient for variety identification, promotion,

Method used

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  • Characteristic sequence, primers and identification method of Carya illinoinensis varieties Nacono and Sumner
  • Characteristic sequence, primers and identification method of Carya illinoinensis varieties Nacono and Sumner
  • Characteristic sequence, primers and identification method of Carya illinoinensis varieties Nacono and Sumner

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Embodiment 1

[0038] (1) Extraction of Genomic DNA of Thin-shelled Hickory Nut Variety:

[0039] Take 0.04g of the young leaves of the pecan variety to be tested, add liquid nitrogen and grind them thoroughly, and use the SDS-CTAB method to extract the genomic DNA. thing. The crude DNA was purified by Magabio Nucleic Acid Purification Kit (Bioer, Hangzhou, China), and detected by 1.5% agarose gel electrophoresis and DNA / RNA UV spectrophotometer (GeneQuant Pro, GE Healthcare). Integrity, Purity and Concentration. DNA samples with OD260 / OD280>1.8 were used for subsequent PCR amplification. DNA extracts were stored in a -20°C refrigerator for later use.

[0040] (2) Design specific PCR amplification primers, the sequence of the primer pair is:

[0041] The upstream primer: 5'-ACTGGGCCTAATCGGTTTGG-3'; and the downstream primer: 5'-CACTAGCACCCCATGAAGCA-3' were synthesized by Shanghai Bioengineering Technology Co., Ltd.

[0042] (4) PCR amplification:

[0043] Composition of PCR reaction so...

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Abstract

The invention relates to a characteristic sequence and a pair of high-specificity molecular labeling primers of Carya illinoinensis varieties Nacono and Sumner, and a method for rapidly identifying the Carya illinoinensis varieties Nacono and Sumner, wherein the sequences of the molecular labeling primers comprise that the upstream primer is 5'-ACTGGGCCTAATCGGTTTGG-3', and the downstream primer is5'-CACTAGCACCCCATGAAGCA-3'. According to the present invention, with the specific molecular labeling primers, the Carya illinoinensis varieties Nacono and Sumner can be rapidly and early identified;and the method is simple, rapid and accurate, and is the irreplaceable molecular method for the apparent characteristic discrimination of the Carya illinoinensis varieties Nacono and Sumner.

Description

(1) Technical field [0001] The invention relates to the characteristic sequence of pecan varieties Nacono and Sumner, a molecular specific marker primer and a method for quickly identifying the pecan varieties Nacono and Sumner by using the molecular specific marker primer. (2) Background technology [0002] Shelled Pecans (Carya (Wangenh.) K.Koch) is the most economically valuable tree species in the Juglandaceae Hickory genus. Carya carya is a typical outcrossing plant. According to the existing production practice, the variety configuration of carya carya is one of the main factors affecting its yield. The U.S. is one of the origins of pecans and its central production area. Over the years, it has selected and named about 1,000 cultivars. It has been more than 100 years since the introduction of hickory nuts in my country, and there are dozens of varieties commonly used in production at present. Years of production practice have proved that the vast subtropical regions...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895
Inventor 朱汤军彭华正金群英叶华琳
Owner ZHEJIANG FORESTRY ACAD
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