Complete ITS sequence of dictyophora indusiata, dictyophora duplicate and dictyophora phalloidea and application in identifying variety of dictyophora
A technology of Dictyophora with short skirt and Dictyophora with long skirt, which is applied in the field of molecular identification methods of its varieties, can solve the problems of species confusion, inability to comprehensively and objectively identify Dictyophora varieties, and affect the use value of Dictyophora and the interests of consumers , to achieve the effect of reliable technology
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Embodiment 1
[0029] Example 1 The ITS1-5.8S-ITS2 region sequence of wild Dictyophora strain ZST was used to identify Dictyophora species.
[0030] In this example, the wild bamboo fungus strain ZST (collected by the patent declaration laboratory) was taken, and the identification was carried out through the following steps.
[0031] (1) Preparation of wild Dictyophora strain ZST mycelium.
[0032] Inoculate the wild bamboo fungus strain ZST strain into PDA liquid medium, and cultivate it at a constant temperature of 25°C. When the mycelium grows to an appropriate amount, collect the mycelium and wash the liquid medium contained in the liquid on the mycelium with sterile distilled water. Suck the water in the mycelium with sterile filter paper, put it into a 1.5 mL centrifuge tube, and freeze it at -20°C for later use.
[0033] (2) Extraction of genomic DNA.
[0034] Weigh 1.5 g of mycelia stored at -20°C into a sterilized and frozen mortar, add an equal volume of quartz sand to grind; tr...
Embodiment 2
[0045] Example 2 The sequence of the ITS1-5.8S-ITS2 region of the wild Dictyophora strain YZS was used to identify Dictyophora species.
[0046] In this example, the wild bamboo fungus strain YZS (collected by the patent declaration laboratory) is taken, and the method is the same as in Example 1. The ITS1-5.8S-ITS2 region sequence (SEQ NO.5) of the strain was compared with the homologous sequences of SEQ NO.1, SEQ NO.2 and SEQ NO.3 using DNAMAN software, and it was found that SEQ NO. .5 and SEQ NO.1, SEQ NO.2 and SEQ NO.3 sequence similarities are 97.35%, 99.81% and 99.62%, respectively, so the wild Dictyophora strain YZS is identified as Dictyophora kiltica.
Embodiment 3
[0047] Example 3 The sequence of the ITS1-5.8S-ITS2 region of the wild Dictyophora strain ZZC2 was used to identify Dictyophora species.
[0048] In this example, the wild bamboo fungus strain ZZC2 (collected by the laboratory for patent application) is used, and the method is the same as in Example 1. The ITS1-5.8S-ITS2 region sequence (SEQ NO.6) of the strain was compared with the homologous sequences of SEQ NO.1, SEQ NO.2 and SEQ NO.3 using DNAMAN software, and it was found that SEQ NO. .6 The sequence similarities with SEQ NO.1, SEQ NO.2 and SEQ NO.3 are 97.17%, 99.62% and 99.81%, respectively, so the wild Dictyophora strain ZZC2 is identified as Dictyophora clavium.
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