Methods and systems for controlling dna, RNA and other biological molecules passing through nanopores

Abstract

The present disclosure provides, in one aspect, a device and a method for unit sequencing and/or analysis of a molecular sequence comprising attaching the molecular sequence to a plate and controllingthe progression of the molecular sequence through a pore of a nanopore chip, wherein the separation distance between the nanopore chip and the scan plate is controlled by a precision mechanical drive, and the molecular sequence is sensed as it progresses through the nanopore.

Description

technical field [0001] The present disclosure generally relates to methods of controlling the movement of molecules, such as DNA (deoxyribonucleic acid), RNA (ribonucleic acid), and proteins, among other biomolecules, through nanopores so that they can be detected, characterized, and / or sequenced. Background technique [0002] Since 454Life Sciences introduced the first second-generation DNA sequencing system in 2005, DNA sequencing has become one of the hottest technologies in the rapidly growing market. The main feature of second-generation DNA sequencing is its massively parallel sequencing capability with ultra-high throughput and short read length. It is faster and cheaper than the first generation technique, the Sanger method. However, the short read lengths of second-generation DNA sequencing make de novo assembly, genome-wide phasing, and structural variation analysis very difficult, limiting its ability to resolve complex DNA regions with repetitive or heterozygous...

AI Technical Summary

Problems solved by technology

This is good for academic research, but not enough for large-scale cheap high-throughput DNA sequencing and other commercial applications

The electro-mechanical approach proposed by Peng et al. (2011) uses a piezoelectric laye

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