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Method for building renal inducible inherent macrophage infiltration mouse model

A technology of macrophages and mouse models, applied to other methods of inserting foreign genetic materials, interleukins, chemical instruments and methods, etc., can solve the single factor that cannot be used as a research model of renal intrinsic macrophages and cannot function in the kidney Analysis and other issues to achieve the effect of avoiding non-selective gene overexpression and non-selective cell infiltration, improving specificity and reducing influence

Active Publication Date: 2018-08-07
TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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Problems solved by technology

[0004] 1) Since this mouse is not a kidney-specific IL34 knockout mouse, let alone a kidney-specific IL34 overexpression mouse, it involves the comprehensive effects of multiple organs and systems, and there is an interaction between each system, so it is impossible to intuitively understand the kidney. One-way analysis of the effect;
[0005] 2) This mouse is also involved in the comprehensive effects of other immune organs, immune cells and other macrophage subtypes, so it cannot be used as a research model for intrinsic macrophages in the kidney

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  • Method for building renal inducible inherent macrophage infiltration mouse model
  • Method for building renal inducible inherent macrophage infiltration mouse model
  • Method for building renal inducible inherent macrophage infiltration mouse model

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Embodiment Construction

[0033] The principles and features of the present invention are described below in conjunction with the accompanying drawings, and the examples given are only used to explain the present invention, and are not intended to limit the scope of the present invention.

[0034] The construction process of the mouse model is as follows: figure 1 shown, including the following steps:

[0035] S1: Construction of LSL-IL34 mice with the IL34 conditional expression cassette;

[0036] S2: Constructing a KSP-iCreERT2 mouse with an active Cre recombinase expression cassette driven by the renal tubular tissue-specific promoter under induction conditions;

[0037] S3: The LSL-IL34 mouse was crossed with the KSP-iCreERT2 mouse, and the offspring mice obtained were the kidney-induced intrinsic macrophage infiltration mouse model.

[0038] 1. Construction of targeting vector

[0039] The schematic diagram of the first targeting carrier is as follows: figure 2 Shown, from 5' to 3' are CAG pr...

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Abstract

The invention provides a method for building a renal inducible inherent macrophage infiltration mouse model. The method includes the step of specifically expressing IL34 genes in mouse renal tubule cells under the condition of induction. IL34 mice are highly expressed by building an inducible renal tubule, so that the renal mouse model taking inherent macrophages as a main infiltration characteristic is successfully built, non-selective gene over-expression and non-selective cell infiltration can be effectively avoided, the influence on extra-renal vital organs and the total state of a body isdecreased, and renal inherent macrophage infiltration specificity can be effectively improved. Besides, by the aid of the model, the function of the renal inherent macrophage can be further researched, a reliable path is provided for seeking inherent macrophage biological markers with more specificity, and a reliable in-vivo study model is provided for drug screening of the targeted renal inherent macrophage.

Description

technical field [0001] The invention relates to the field of mouse disease models, in particular to a method for constructing a kidney inducible intrinsic macrophage infiltration mouse model. Background technique [0002] Macrophages are highly heterogeneous. During the resting period of the kidney, they maintain tissue homeostasis and tissue repair; in pathological conditions, they promote renal fibrosis. Most previous studies simply divided macrophages into M1 (inflammatory macrophages) and M2 (reparative macrophages), ignoring the phenotype, function, connotation and environmental response of macrophages. This complexity, while ignoring the phenomenon of M1 / M2 functional crossover, has led to ambiguity about the role of macrophages in kidney disease. Therefore, more and more researchers now advocate that macrophages in tissues can be divided into peripheral macrophages and intrinsic macrophages (Tissue-resident macrophages) according to their origin. Peripheral macropha...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/90C12N9/22C12N15/66A01K67/027
CPCA01K67/0275A01K2227/105A01K2267/03C07K14/54C12N9/22C12N15/66C12N15/907
Inventor 曾锐廖文慧
Owner TONGJI HOSPITAL ATTACHED TO TONGJI MEDICAL COLLEGE HUAZHONG SCI TECH
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