Application of probiotics and outer membrane vesicles thereof to preparing medicines for preventing and treating osteoporosis

A technology for osteoporosis and adventitial vesicles, which can be used in applications, drug combinations, pharmaceutical formulations, etc., to solve problems such as atypical fractures and osteonecrosis of the mandible, and achieve easy preservation, good curative effects, and reduced incidence of side effects. Effect

Active Publication Date: 2018-08-10
湖南鹤螈生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current drugs for osteoporosis are mainly bisphosphonates, but there are side effects such as mandibular osteonecrosis and atypical fractures
There is no report that Akk bacteria can improve osteoporosis, especially postmenopausal osteoporosis
At present, there is no report on the direct application of probiotics to OMV to intervene in osteoporosis

Method used

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  • Application of probiotics and outer membrane vesicles thereof to preparing medicines for preventing and treating osteoporosis
  • Application of probiotics and outer membrane vesicles thereof to preparing medicines for preventing and treating osteoporosis
  • Application of probiotics and outer membrane vesicles thereof to preparing medicines for preventing and treating osteoporosis

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Experimental program
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Embodiment 1

[0049] (1) Acquisition and cultivation of Akk bacteria:

[0050] Acquisition and cultivation of Akk bacteria: We purchased Akk strains from ATCC (American type culture collection; American Type Culture Collection Bank), and activated the bacteria according to the instructions on the official website: Brain Heart Infusion Broth (Brain Heartlnfusion Broth; BHI; Haibo Biological) 38.5g, add double distilled water 950ml, and sterilize at 121°C. After it cools down, add 50ml of defibrated sheep blood (Haibo Biology) under aseptic conditions, add 0.3‰ of L-cysteine ​​(McLean) and 1% resazurin (Soleibao) after mixing, Put it in a sterile environment overnight to make the medium fully free from oxidation. Dissolve Akk bacteria powder with about 0.5ml of BHI medium, transfer to 50ml of BHI medium, and subculture for 3-4 days under anaerobic environment at 37°C with shaking at 170rpm. All the above operations need to be carried out in a sterile environment.

[0051] (2) Extraction of...

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Abstract

The invention discloses application of probiotics or outer membrane vesicles thereof to preparing medicines for preventing and treating osteoporosis. The probiotics is an Akk bacterium. The probioticsor the outer membrane vesicles of the probiotics can be used for increasing the bone volume fractions of distal-end cancellous bones of femurs, increasing the numbers of bone trabeculas and loweringthe separation of the bone trabeculas. The probiotics or the outer membrane vesicles of the probiotics can be used for preparing the medicines, food or healthcare products for preventing and treatingpostmenopausal osteoporosis. The application has the advantage that novel, safe and effective biotherapy directions can be provided to treating the osteoporosis and particularly the postmenopausal osteoporosis by the application.

Description

technical field [0001] The present invention relates to the technical field of microbial application, in particular to the application of probiotics and their outer membrane vesicles in the preparation of medicines for preventing and treating osteoporosis, in particular to Akkermansia muciniphila bacteria (Akkermansia muciniphila) Mannella, ATCC BAA-835, hereinafter referred to as Akk bacteria) and its outer membrane vesicles (outer membrane vesicles; OMVs) in the preparation of drugs for the prevention and treatment of osteoporosis. Background technique [0002] Osteoporosis is a metabolic bone disease characterized by decreased bone mass and decreased bone fragility, which often induces fractures and brings a heavy burden to individuals, families and society. Divided into primary and secondary two categories, of which primary osteoporosis is divided into postmenopausal osteoporosis, senile osteoporosis and idiopathic osteoporosis. According to statistics from the Internat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/741A61P19/10A61P15/12A23L33/135
CPCA23L33/135A23V2002/00A61K35/741A61P15/12A61P19/10A23V2200/306
Inventor 谢辉刘江华刘征兆罗忠伟谭艺娟殷豪黄杰罗娟
Owner 湖南鹤螈生物科技有限公司
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