Micro-fluidic chip for cell migration and preparation method of micro-fluidic chip

A microfluidic chip, cell migration technology, used in biochemical equipment and methods, tissue cell/virus culture devices, enzymology/microbiology devices, etc.

Active Publication Date: 2018-08-10
NORTHWESTERN POLYTECHNICAL UNIV
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there is no report on the microfluidic chip and its preparation method that combines cell microenvironment simulation and biochemical factor / drug concentration gradient to study cell migration

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Micro-fluidic chip for cell migration and preparation method of micro-fluidic chip
  • Micro-fluidic chip for cell migration and preparation method of micro-fluidic chip
  • Micro-fluidic chip for cell migration and preparation method of micro-fluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A microfluidic chip for cell migration, such as figure 1 As shown, the chip is provided with a flow layer 1, a control layer 2, a film layer 3 and a glass layer 4 from top to bottom; the film layer 3 is used for sealing the control layer 2 and the glass layer 4; the flow layer 1 is composed of A pyramidal network structure 6, a cell culture area 8, a cell migration area 9, a main pipeline 10, a first liquid inlet micropipe 5, and a second liquid inlet micropipe 7; the control layer 2 is composed of the first microvalve 11, the second microvalve The second microvalve 12, the third microvalve 13, the fourth microvalve 14, and the fifth microvalve 15 are composed of several microvalves.

[0032] like figure 2 As shown, the pyramidal network structure 6 comprises 4 rows of serpentine micro-pipes 16, and each row from top to bottom comprises 3, 4, 5, and 6 serpentine micro-pipes 16, and each row is connected to the adjacent row of serpentine micro-pipes 16. The serpentine...

Embodiment 2

[0046] The substance concentration gradient mode is established in the main pipeline 10 as Figure 4 shown. Drive the first micro-valve 11 with the gas pressure of 15 psi, close the second liquid inlet micro-pipeline 7; Inject two kinds of water into the serpentine micro-pipeline 16 of the pyramidal network structure 6 respectively through the two entrances of the first liquid-feed micro-pipeline 5. solution, the liquid perfusion flow rate is 0.5 μL / min; the two solutions form different concentrations through continuous diversion, convergence and mixing, and then flow into the main channel 10 , and finally establish a substance concentration gradient in the main channel 10 .

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
widthaaaaaaaaaa
widthaaaaaaaaaa
lengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a micro-fluidic chip for cell migration and a preparation method of the micro-fluidic chip. The micro-fluidic chip is provided with a flow layer, a control layer, a film layerand a glass layer from top to bottom; the flow layer consists of a pyramid-shaped network structure, a cell culture zone, a cell migration zone, a main pipeline and a liquid inlet micro pipeline; andthe control layer consists of a plurality of micro valves. The invention also provides a preparation method of the micro-fluidic chip. A biochemical factor / medicine concentration gradient is formed inthe main pipeline and the cell migration area, the effect of the biochemical factors / medicine of different concentrations for the cell migration can be researched in a same chip, and the conventionalmode for researching the effect of the single-concentration biochemical factor / medicine for the cell migration is broken through. The invention can also realize the environment construction of an extracellular matrix and co-culture of target cells and matrix cells, and the micro-fluidic chip is used for researching the haptotaxis of the extracellular matrix and the matrix cell for the target cell. Therefore, the cell migration can be researched by combining the biochemical factor / medicine concentration gradient and cell micro environment simulation.

Description

technical field [0001] The invention relates to a microfluidic chip and a preparation method thereof, in particular to a microfluidic chip and a preparation method thereof for studying cell migration in combination with cell microenvironment simulation and biochemical factor / drug concentration gradient. Background technique [0002] Microfluidics integrates the basic operating units of sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes into a chip of a few square centimeters, and automatically completes the entire analysis process. Due to its great potential in the research and application of biology, chemistry, medicine and other fields, it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, machinery and so on. At present, widely used biochips (micro-arrays), such as gene chips and protein chips, are just dot-array hybridization chips with zero...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/16
Inventor 任丽商澎武婉情
Owner NORTHWESTERN POLYTECHNICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap