A microfluidic chip for cell migration and its preparation method

A microfluidic chip and cell migration technology, applied in biochemical equipment and methods, tissue cell/virus culture devices, enzymology/microbiology devices, etc.

Active Publication Date: 2021-09-10
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the prior art, there is no report on the microfluidic chip and its preparation method that combines cell microenvironment simulation and biochemical factor / drug concentration gradient to study cell migration

Method used

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  • A microfluidic chip for cell migration and its preparation method
  • A microfluidic chip for cell migration and its preparation method
  • A microfluidic chip for cell migration and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A microfluidic chip for cell migration, such as figure 1 As shown, the chip is provided with a flow layer 1, a control layer 2, a film layer 3 and a glass layer 4 from top to bottom; the film layer 3 is used for sealing the control layer 2 and the glass layer 4; the flow layer 1 is composed of A pyramidal network structure 6, a cell culture area 8, a cell migration area 9, a main pipeline 10, a first liquid inlet micropipe 5, and a second liquid inlet micropipe 7; the control layer 2 is composed of the first microvalve 11, the second microvalve The second microvalve 12, the third microvalve 13, the fourth microvalve 14, and the fifth microvalve 15 are composed of several microvalves.

[0032] Such as figure 2 As shown, the pyramidal network structure 6 comprises 4 rows of serpentine micro-pipes 16, and each row from top to bottom comprises 3, 4, 5, and 6 serpentine micro-pipes 16, and each row is connected to the adjacent row of serpentine micro-pipes 16. The serpent...

Embodiment 2

[0046] The substance concentration gradient mode is established in the main pipeline 10 as Figure 4 shown. Drive the first micro-valve 11 with the gas pressure of 15 psi, close the second liquid inlet micro-pipeline 7; Inject two kinds of water into the serpentine micro-pipeline 16 of the pyramidal network structure 6 respectively through the two entrances of the first liquid-feed micro-pipeline 5. solution, the liquid perfusion flow rate is 0.5 μL / min; the two solutions form different concentrations through continuous diversion, convergence and mixing, and then flow into the main channel 10 , and finally establish a substance concentration gradient in the main channel 10 .

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Abstract

The invention discloses a microfluidic chip for cell migration and a preparation method thereof. A flow layer, a control layer, a film layer and a glass layer are arranged from top to bottom; the flow layer consists of a pyramidal network structure, a cell culture area, The cell migration area, the main pipe and the liquid inlet micropipe are composed; the control layer is composed of several microvalves. The invention also provides a preparation method of the microfluidic chip. The present invention can form a biochemical factor / drug concentration gradient in the main pipeline and the cell migration area, which is beneficial to study the effects of different concentrations of biochemical factors / drugs on cell migration in the same chip, and breaks through the usual single-concentration biochemical factor / drug effect on cells. Research model of migration. The invention can also realize the construction of the microenvironment of the extracellular matrix and the co-cultivation of the target cells and the stromal cells, and is used for the research on the haptotaxis of the extracellular matrix and the stromal cells to the target cells. It can be seen that the present invention can combine biochemical factor / drug concentration gradient and cell microenvironment simulation to study cell migration.

Description

technical field [0001] The invention relates to a microfluidic chip and a preparation method thereof, in particular to a microfluidic chip and a preparation method thereof for studying cell migration in combination with cell microenvironment simulation and biochemical factor / drug concentration gradient. Background technique [0002] Microfluidics integrates the basic operating units of sample preparation, reaction, separation, and detection in biological, chemical, and medical analysis processes into a chip of a few square centimeters, and automatically completes the entire analysis process. Due to its great potential in the research and application of biology, chemistry, medicine and other fields, it has developed into a new research field interdisciplinary in biology, chemistry, medicine, fluid, electronics, materials, machinery and so on. At present, widely used biochips (micro-arrays), such as gene chips and protein chips, are just dot-array hybridization chips with zero...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M23/16
Inventor 任丽商澎武婉情
Owner NORTHWESTERN POLYTECHNICAL UNIV
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