Sex hormone-binding globulin preservation solution and its application
The technology of binding globulin and preservation solution is applied in the application field of sex hormone binding globulin preservation solution and preservation solution, and can solve the problems of poor liquid stability of sex hormone binding globulin, unstable sex hormone binding globulin, inaccurate inspection results, etc. To achieve the effect of high stability of sex hormone binding globulin structure and activity, stable concentration and stable biological activity
Active Publication Date: 2021-08-17
SHENZHEN NEW INDS BIOMEDICAL ENG
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Problems solved by technology
[0009] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art, and provide a preservation solution of sex hormone-binding globulin to solve the technical problem of difficult preservation caused by the poor liquid stability of existing sex hormone-binding globulin
[0010] Another object of the present invention is to provide a kind of calibrator and quality control product and test kit for detecting sex hormone-binding globulin, to solve the existing sex hormone-binding globulin liquid calibrator and quality control product due to lack of sex hormone-binding globulin Stable technical issues that lead to inaccurate inspection results
Method used
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Effect test
Embodiment 11
[0056] A SHGB preservation solution, its specific preparation method is: add 50ml goat serum, 1mlTween-20, 102ml glycerin, 6.66g CaCl to the beaker successively 2 , 17.55g NaCl, 75.07g glycine, 5g BSA, 1g NaN 3 After 1.5gProclin 300, add 500ml 0.12M trishydroxymethylaminomethane (TRIS) to dissolve and stir, then add 5ml 1mM N,N-dimethylformamide (DMF) dissolved dihydrotestosterone mother solution, add dilute hydrochloric acid to adjust the solution pH value to 7.4, and finally add water to dilute to 1L.
[0057] The components of this preservation solution are:
[0058]
Embodiment 12
[0060] A SHGB preservation solution, its specific preparation method is the same as in Example 1, only the mother solution of 0.5% v / v 1mM dihydrotestosterone is replaced by the mother solution of 0.5% v / v 1mM estradiol.
Embodiment 13
[0062] A kind of SHGB storage solution, its specific preparation method is the same as embodiment 1, and the component of this storage solution is:
[0063]
[0064]
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Abstract
The invention discloses a preservation solution of sex hormone-binding globulin, a preparation method thereof, a detection agent and a kit for detecting sex hormone-binding globulin, and belongs to the technical field of in vitro diagnostic reagents. The preservation solution includes a buffer, sex hormones, IIA and / or IIB family ions, osmotic agents, animal serum, surfactants and protein stabilizers, wherein the concentration of the sex hormones in the preservation solution is 1- 10 μM, the concentration of the group IIA and / or group IIB ions in the preservation solution is 10-60 mM. Both the detection agent and the kit contain the preservation solution of the present invention. The preservation solution of the sex hormone binding globulin of the present invention can keep the dimer structure and biological activity of the sex hormone binding globulin dissolved in the preservation solution stable. The detection agent and kit can ensure the detection accuracy of sex hormone binding globulin, and are convenient and fast.
Description
technical field [0001] The invention belongs to the technical field of in vitro diagnostic reagents, and in particular relates to a sex hormone-binding globulin preservation solution and the application of the preservation solution. Background technique [0002] Sex hormones are regulated by the pituitary gland and the hypothalamus. There is a complex relationship between the hypothalamus, pituitary gland, and gonadal hormones that are interconnected and mutually restricted. They participate in the control and regulation of reproductive activities, which is called the hypothalamus-pituitary-gonad axis. The androgen is mainly composed of testosterone synthesized and secreted by the testis, which is a steroid hormone; in addition, the adrenal cortex and the ovary of women can also secrete a small amount of androgen. The main physiological role of androgens is to promote the formation of male reproductive organs and the development of secondary sexual characteristics, promote t...
Claims
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IPC IPC(8): C07K14/47G01N33/74
CPCC07K14/4717C07K2319/70G01N33/743
Inventor 饶微袁锦云肖泽林张扬王小莉陈曙光
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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