Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A method for regulating cell pathways by using plant hormone GA and small molecular substance pac

A technology of small molecular substances and plant hormones, applied in the field of biological method invention, can solve the problems of immune system damage and incompatibility

Inactive Publication Date:
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, both this method and optogenetics have certain limitations
Rapamycin is an immunosuppressor, which has certain damage to the immune system; the optogenetics system is not suitable for the regulation of intracellular light signaling pathways

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A method for regulating cell pathways by using plant hormone GA and small molecular substance pac
  • A method for regulating cell pathways by using plant hormone GA and small molecular substance pac
  • A method for regulating cell pathways by using plant hormone GA and small molecular substance pac

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037]A method for regulating cell pathways by utilizing the plant hormone GA and the small molecular substance PAC, comprising the following methods:

[0038]

[0039] (1) Carrier design and construction:

[0040] First, using the primers described above, using Arabidopsis thaliana cDNA and HEK293T cell cDNA as templates, PCR technology is used to amplify each gene fragment, and then using the corresponding primers, using two fragments as templates, carry out overlap PCR; The Arabidopsis DELLA protein GAI and the GA receptor GID1 were fused with the C-terminus of the key protein LRP6 in the Wnt signaling pathway, and the PCR products such as GAI, GID1, GAI-LRP6C, GID1-LRP6C were recovered by agarose gel, and the internal The linearized pCI 4xmyc, pCI egfp and pCI mcherry vectors were digested with XbaI and XmaI enzymes, ligated by Infusion, transformed into DH5a, verified by colony pcr, picked and sent for sequencing, and ensured that the bacteria were correctly sequenced;...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for regulating cell pathway by using plant hormone GA and small molecular substance PAC, and realizes the regulation switch of signal pathway in human cells. The GA receptor proteins GID1 and GAI in Arabidopsis thaliana were constructed on a human cell expression vector, and then transferred into HEK293T cells together. GA was added to induce the interaction between the two proteins. After adding the inhibitor PAC, the degree of interaction would be weakened. In this way, the key proteins in the human cell signaling pathway are fused and expressed with these two receptor proteins. The two receptor proteins interact under the induction of GA, and the fusion signaling pathway is also induced by GA. The present invention selects the key protein LRP6 on the human Wnt pathway. After the fusion expression of GA receptor and LRP6, LRP6 will aggregate under the induction of GA, thereby promoting the production of β-catenin and opening the intracellular signaling pathway. After adding PAC inhibition, the production of β-catenin is reduced and the cell pathway is closed.

Description

technical field [0001] The invention relates to the field of biological method inventions, specifically, a method for regulating cell pathways by using plant hormone GA and small molecular substance PAC. Background technique [0002] Optogenetics is a new technology that combines light control technology with genetics for cell biology research, that is, expresses light-sensitive ion channel proteins on excitable target cells or organs, and activates them with light of corresponding wavelengths. Light-sensitive channels are used to fine-tune the physiological functions of cells, tissues, organs and animals. So far, many photoreceptors have been improved and used to fuse key proteins in signaling pathways to regulate and control many pathways in organisms. Such as receptor tyrosine kinase Ras-MAPK signaling pathway, PI3K-Akt signaling pathway and Wnt signaling pathway. At the gene level, the researchers used elements such as CRY2-CIB1, FKF1-GI, UVR8-COP1, and LOV proteins to ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/85C12N5/10
CPCC12N15/85C12N2840/002
Inventor 左泽乘张峻川莫伟亮张力肖勇石翔张宝昌张文礼李苏迪
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com