Boar StAR gene 5-bp repetitive deletion polymorphism detection method and application

Abstract

The invention discloses a boar StAR gene 5-bp repetitive deletion polymorphism detection method and application. The method includes: taking to-be-detected boar whole-genome DNA as a template to amplify a StAR gene segment by means of PCR (polymerase chain reaction); performing agarose gel electrophoresis, and authenticating 5-bp repetitive deletion polymorphism of StAR gene at NC_010457.5:g.5524-5528 locus according to an electrophoresis result. Different genotypes of StAR gene 5-bp repetitive deletion polymorphism are remarkably associated with reproductive traits including testicle long axis length, testicle short axis length, testicle weight and the like of large white boars at ages of 15 days and 40 days. By a boar StAR gene insertion / deletion method, application in marker assisted selective breeding of boar reproductive traits can be realized, and boar genetic resource groups excellent in reproductive performance can be quickly established.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion (indel), in particular to a method for rapidly and accurately detecting boar StAR gene NC_010457.5:g.5524-5528 site 5-bp repeat deletion multiple method and its application. Background technique [0002] With the improvement of human living standards, the consumer demand for livestock, especially pigs (Sus scrofa), is getting higher and higher, and the pig breeding industry has developed rapidly. China is a big pork consumer, and pork accounts for more than 60% of the total meat consumption. According to relevant statistics, in China, the annual per capita pork consumption is about 59 kilograms, which means that Chinese consumers account for more than half of the world's pork consumption. Therefore, cultivating germplasm resources with excellent traits is the guarantee for sufficient pork supply. [0003] With the devel...

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Problems solved by technology

[0010] At present, the research on StAR gene mainly focuses on human-related diseases, but the research on the association between the genetic variation of StAR gene and the reproductive traits of boars is very scarce.

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