Particle track analysis method and device with sample liquid level stability detection function

Abstract

The invention discloses a particle track analysis method and device with a sample liquid level stability detection function, and belongs to the field of nano-particle particle size measurement. A dual-frequency interference measurement technology is fused into a nano-particle tracking analysis technology, o light in a dual-frequency interference measurement light path enters a floating plate plated with a reflecting film in a sample cell, e light enters a planar spectroscope fixed to the top of the sample cell, transmission light enters a sample solution area on the lower portion of the planarspectroscope and then is reflected into a CCD (charge coupled device) microscope lens particle track measurement analysis area by an oblique lateral reflecting surface of the sample cell, and a lightsource field is provided for particle track analysis. After the o light and the e light reflected by the floating plate and the planar spectroscope are mixed at beat frequency, beat frequency signalscontain information of the floating pendulum degree of the floating plate, the static stability state of sample solution in the nano-particle tracking analysis process is detected, particularly, micro-vibration of the sample solution is detected, and necessary technical means is provided for ensuring nano-particle tracking analysis accuracy.

Description

technical field [0001] The invention belongs to the field of particle size measurement of nanoparticles, and mainly relates to a method and a device for realizing accurate tracking and analysis of tracks of nanoparticles. Background technique [0002] The characteristics of nanomaterials in the fields of materials, biology, medicine, environmental protection, chemical industry, metallurgy and construction are closely related to their particle concentration and particle size distribution. Nanoparticle measurement technology has received more and more attention, and has great economic and far-reaching social significance for improving the quality of material products, biomedicine, and controlling environmental pollution. Nanoparticle tracking analysis (NTA method) is a particle size measurement method that was just commercialized in 2006. This method is based on laser illumination microscopy technology, through fixed-wavelength laser illumination and a certain magnification ch...

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Problems solved by technology

[0003] In order to obtain the accurate Brownian motion trajectory of the particles in the sample solution, it is necessary to ensure that the sample solution is in a static and stable state. The placement of the sample solution in the initial stage of measurement and analysis and the external vibration during the measurement and analysis will make the sample solution in a non-static and stable state. Whether the solution is in a static and stable state is realized by visual judgment, and there is a lack of effective detection means and methods for whether the sample solution is in a static and stable state, especially the technical means for detecting the small vibration of the sample solution

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