Method for detecting meat freshness through ultra-weak biophoton imaging system
An imaging system and biophotonic technology, applied in the fields of food safety detection and biomedical technology imaging, can solve the problems of long experiment period, difficult to judge the color change of the titration end point, difficult to clean the instrument, etc., and achieve the effect of easy operation.
Inactive Publication Date: 2018-08-17
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology
Physical and chemical testing methods are commonly used to detect food freshness. Its indicators usually include volatile basic nitrogen, catalase, pH value and globulin precipitation reaction, etc. However, the current national standard method for testing meat freshness is only the detection of The method of volatile basic nitrogen, which can detect meat of different freshness, distinguish fresh meat, sub-fresh meat and spoiled meat, and is consistent with sensory inspection, but its detection equipment is complicated, the operation is cumbersome and time-consuming, and it needs to use Distillation and titration equipment, the experiment period is relatively long, the color change of the titration end point is not easy to judge, and it is also found that it is not sensitive to the change of the freshness of beef during the experiment operation, and skilled technicians are required to obtain a more reliable judgment result , and it is difficult to clean the instrument, and it is difficult to perform rapid detection on the spot
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[0036] Example: Ultraweak Biophotonic Detection of Beef Freshness
[0037] 1. Experimental materials:
[0038] Beef (adult cattle, purchased at the cold fresh meat wholesale market on Xinzhu Road, Hongshan District, Wuhan)
[0039] 2. Experimental steps
[0040] S1, the pretreatment of material, comprises the following steps:
[0041] S11. Take out a portion of fresh beef from the 4°C refrigerator, accurately weigh 0.2000g of beef with an electronic balance, cut it into pieces with scissors, and place it in a glass grinder;
[0042] S12. Add 400 μL of 1×PBS buffer solution to the glass grinder, place it on ice and grind it for 5 minutes to obtain the first homogenate and store it in a refrigerator at 4°C for later use;
[0043] S13, the same step as above, weigh the same beef and then prepare the second homogenate, the third homogenate, the fourth homogenate and the fifth homogenate, the second homogenate, the third homogenate, the fourth homogenate and the fifth homogenate...
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The invention discloses a method for detecting meat freshness through an ultra-weak biophoton imaging system. The method includes steps: B1, material pretreatment; B2, experiment preparation and imaging system preheating; B3, experiment starting; B4, positioning and shooting; B5, experimental imaging; B6, sequence image processing and data analysis; B7, other meat sample detection. Distinctive biophoton radiation variation of meat along with variation of storage time can be used for quick and qualitative determination of meat freshness, namely fresh meat, second-rate fresh meat and deteriorated meat. The method has advantages of simplicity and convenience in operation, quickness and the like and can be widely applied to fields of life science research, food safety, environmental protectionand the like.
Description
technical field [0001] The invention relates to the fields of food safety detection and biomedical technology imaging, and can also be applied to other fields that need to detect ultra-weak bioluminescence, and in particular relates to a method for detecting the freshness of meat through an ultra-weak biophoton imaging system. Background technique [0002] Biological ultra-weak luminescence, referred to as biophoton, is a very common phenomenon in living organisms, also known as ultra-weak photon radiation (uitraweakphoton emission, UPE), which is a natural manifestation of living organisms. The radiation intensity is very small and the spectral range Usually about 200nm ~ 800nm, but occasionally to the infrared band. UPE widely exists in plants, animals and single-celled organisms, and it reflects the origin of organisms and information related to life activities. The source of biophotons is mainly the metabolic activities of biological tissues, such as oxidative metabolis...
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Login to View More IPC IPC(8): G01N21/63
CPCG01N21/63
Inventor 戴甲培李颖越李泽华肖芳艳
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES